Silver nanoparticles induced changes in the expression of NF-kappaB related genes are cell type specific and related to the basal activity of NF-kappaB.
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Stepkowski TM, Brzoska K, Kruszewski M
Silver nanoparticles induced changes in the expression of NF-kappaB related genes are cell type specific and related to the basal activity of NF-kappaB.
Toxicol In Vitro. 2014 Jun;28(4):473-8. doi: 10.1016/j.tiv.2014.01.008. Epub 2014 Jan 23.
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- 24462830 [ View in PubMed]
- Abstract
Silver nanoparticles (AgNPs) are widely used in industry and medicine but the recent evidence for their cytotoxicity rise a concern about the safety of their use. We have previously shown that human A549 cells are resistant to AgNPs cytotoxicity, as compared with similarly treated HepG2 cells. In order to check for the role of the NF-kappaB signaling pathway in response of A549 and HepG2 cell lines to the treatment with 20 nm and 200 nm AgNps, we analyzed the expression of 84 key genes related to the functionality of the NF-kappaB signaling pathway. We observed considerable alternations in gene expression in HepG2 cells treated with 20 nm AgNPs, and minor changes when exposed to 200 nm AgNPs. Surprisingly, no changes in gene expression were observed in A549 cells treated with both size AgNPs. Using the NF-kappaB luciferase reporter system, we further tested the basal activity and inducibility of the NF-kappaB pathway in both cell lines and found that the inducibility of NF-kappaB signaling in A549 cells is approximately 5 times lower than this of HepG2 cells, but the basal activity is approximately 3.5 times higher. In accordance, the NF-kappaB activation after AgNPs treatment was observed in HepG2 but not in A549. Altogether indicate that NF-kB mediated cellular response to AgNPs is cell type specific and related to the basal activity of NF-kappaB.