[Features of binding of proflavine to DNA at different DNA-ligand concentration ratios].

Article Details

Citation

Copy to clipboard

Berezniak EG, gladkovskaia NA, Khrebtova AS, Dukhopel'nikov EV, Zinchenko AV

[Features of binding of proflavine to DNA at different DNA-ligand concentration ratios].

Biofizika. 2009 Sep-Oct;54(5):805-12.

PubMed ID

19894617 [ View in PubMed

]

Abstract

The binding of proflavine to calf thymus DNA has been studied using the methods of differential scanning calorimetry and spectrophotometry. It was shown that proflavine can interact with DNA by at least 3 binding modes. At high DNA-ligand concentration ratios (P/D), proflavine intercalates into both GC- and AT-sites, with a preference to GC-rich sequences. At low P/D ratios proflavine interacts with DNA by the external binding mode. From spectrophotometric concentration dependences, the parameters of complexing of proflavine with DNA were calculated. Thermodynamic parameters of DNA melting were calculated from differential scanning calorimetry data.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Proflavine	DNA	Nucleotide	Humans	Yes	Intercalation	Details