Development of a novel LC-MS/MS method for the determination of letosteine in human plasma and its application on pharmacokinetic studies.

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Liu X, Zheng H, Tang W, Qian Z, Zhu Y, Wang J, Yuan S, Wen X, Cao C, Chen H

Development of a novel LC-MS/MS method for the determination of letosteine in human plasma and its application on pharmacokinetic studies.

J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Sep 1;879(25):2554-60. doi: 10.1016/j.jchromb.2011.07.011. Epub 2011 Jul 18.

PubMed ID
21840272 [ View in PubMed
]
Abstract

Letosteine has been found to be effective in treating patients with chronic bronchopneumopathies in clinical practice. To provide robust support for its pharmacokinetic and clinical studies, a rapid and sensitive method based on liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was developed and validated for the analysis of letosteine in plasma samples. After protein precipitation, the plasma samples were separated on a reversed-phase C(18) column in less than 1.5 min. The LC-MS/MS system was performed in the positive ion multiple-reaction-monitoring (MRM) mode to produce intensive product ions of m/z 280.1-->160.0 for letosteine and m/z 248.1-->121.1 for the internal standard, tinidazole. The method was found to have excellent linearity (r >/= 0.9974), precision (RSD

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