Uridine phosphorylase

Details

Name
Uridine phosphorylase
Kind
protein
Synonyms
  • 2.4.2.3
  • UPase
Gene Name
udp
UniProtKB Entry
P12758Swiss-Prot
Organism
Escherichia coli (strain K12)
NCBI Taxonomy ID
83333
Amino acid sequence
>lcl|BSEQ0016356|Uridine phosphorylase
MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKLASHREFTTWRAELDGK
PVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHINVGDVLVTTASVRLDGAS
LHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSDTFYPGQERYDTYSGRVVRHF
KGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAGVIVNRTQQEIPNAETMKQTESH
AVKIVVEAARRLL
Number of residues
253
Molecular Weight
27158.88
Theoretical pI
6.16
GO Classification
Functions
identical protein binding / uridine phosphorylase activity
Processes
cellular response to DNA damage stimulus / nucleotide catabolic process / UMP salvage
Components
cytosol / membrane
General Function
Uridine phosphorylase activity
Specific Function
Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Cytoplasm
Gene sequence
>lcl|BSEQ0016357|Uridine phosphorylase (udp)
ATGTCCAAGTCTGATGTTTTTCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACG
CTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCGCTGATGGATAAG
CCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAA
CCTGTTATCGTCTGCTCTACCGGTATCGGCGGCCCGTCTACCTCTATTGCTGTTGAAGAG
CTGGCACAGCTGGGCATTCGCACCTTCCTGCGTATCGGTACAACGGGCGCTATTCAGCCG
CATATTAATGTGGGTGATGTCCTGGTTACCACGGCGTCTGTCCGTCTGGATGGCGCGAGC
CTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTG
GTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGAT
ACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTT
AAAGGTTCTATGGAAGAGTGGCAGGCGATGGGCGTAATGAACTATGAAATGGAATCTGCA
ACCCTGCTGACCATGTGTGCAAGTCAGGGCCTGCGTGCCGGTATGGTAGCGGGTGTTATC
GTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCAT
GCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP12758
UniProtKB Entry NameUDP_ECOLI
GenBank Protein ID43242
GenBank Gene IDX15689
PDB ID(s)1K3F, 1LX7, 1RXC, 1RXS, 1RXU, 1RXY, 1T0U, 1TGV, 1TGY, 1U1C, 1U1D, 1U1E, 1U1F, 1U1G, 3KVV
KEGG IDecj:JW3808
NCBI Gene ID948987
General References
  1. Walton L, Richards CA, Elwell LP: Nucleotide sequence of the Escherichia coli uridine phosphorylase (udp) gene. Nucleic Acids Res. 1989 Aug 25;17(16):6741. [Article]
  2. Daniels DL, Plunkett G 3rd, Burland V, Blattner FR: Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes. Science. 1992 Aug 7;257(5071):771-8. [Article]
  3. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
  4. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
  5. Henzel WJ, Billeci TM, Stults JT, Wong SC, Grimley C, Watanabe C: Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5011-5. [Article]
  6. Gonzalez-Gil G, Bringmann P, Kahmann R: FIS is a regulator of metabolism in Escherichia coli. Mol Microbiol. 1996 Oct;22(1):21-9. [Article]
  7. Veiko VP, Chebotaev DV, Ovcharova IV, Gul'ko LB: [Protein engineering of uridine phosphorylase from Escherichia coli K-12. I. Cloning and expression of uridine phosphorylase genes from Klebsiella aerogenes and Salmonella typhimurium in E. coli]. Bioorg Khim. 1998 May;24(5):381-7. [Article]
  8. Morgunova EYu, Mikhailov AM, Popov AN, Blagova EV, Smirnova EA, Vainshtein BK, Mao C, Armstrong ShR, Ealick SE, Komissarov AA, et al.: Atomic structure at 2.5 A resolution of uridine phosphorylase from E. coli as refined in the monoclinic crystal lattice. FEBS Lett. 1995 Jun 26;367(2):183-7. [Article]
  9. Burling FT, Kniewel R, Buglino JA, Chadha T, Beckwith A, Lima CD: Structure of Escherichia coli uridine phosphorylase at 2.0 A. Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):73-6. Epub 2002 Dec 19. [Article]

Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
5-fluorouridineexperimentalunknowntargetDetails
2'-DeoxyuridineexperimentalunknowntargetDetails
2,8-bis[oxido(oxo)vanadio]-1,1,1,3,5,5,7,7,9,9,9-undecaoxopentavanadoxane-2,8-diiumexperimentalunknowntargetDetails
Ribose-1-PhosphateexperimentalunknowntargetDetails
Thymidineexperimental, investigationalunknowntargetDetails
1-((2-HYDROXYETHOXY)METHYL)-5-(PHENYLTHIO)PYRIMIDINE-2,4(1H,3H)-DIONEexperimentalunknowntargetDetails
1-((2-HYDROXYETHOXY)METHYL)-5-(3-(BENZYLOXY)BENZYL)PYRIMIDINE-2,4(1H,3H)-DIONEexperimentalunknowntargetDetails
5-BenzylacyclouridineexperimentalunknowntargetDetails
1-((2-HYDROXYETHOXY)METHYL)-5-(3-(BENZYLOXY)BENZYL)-6-HYDROXYPYRIMIDINE-2,4(1H,3H)-DIONEexperimentalunknowntargetDetails