RNA-directed RNA polymerase
Details
- Name
- RNA-directed RNA polymerase
- Kind
- protein
- Synonyms
- 2.7.7.48
- Protein P2
- Gene Name
- P2
- UniProtKB Entry
- P11124Swiss-Prot
- Organism
- Pseudomonas phage phi6
- NCBI Taxonomy ID
- 10879
- Amino acid sequence
>lcl|BSEQ0011021|RNA-directed RNA polymerase MPRRAPAFPLSDIKAQMLFANNIKAQQASKRSFKEGAIETYEGLLSVDPRFLSFKNELSR YLTDHFPANVDEYGRVYGNGVRTNFFGMRHMNGFPMIPATWPLASNLKKRADADLADGPV SERDNLLFRAAVRLMFSDLEPVPLKIRKGSSTCIPYFSNDMGTKIEIAERALEKAEEAGN LMLQGKFDDAYQLHQMGGAYYVVYRAQSTDAITLDPKTGKFVSKDRMVADFEYAVTGGEQ GSLFAASKDASRLKEQYGIDVPDGFFCERRRTAMGGPFALNAPIMAVAQPVRNKIYSKYA YTFHHTTRLNKEEKVKEWSLCVATDVSDHDTFWPGWLRDLICDELLNMGYAPWWVKLFET SLKLPVYVGAPAPEQGHTLLGDPSNPDLEVGLSSGQGATDLMGTLLMSITYLVMQLDHTA PHLNSRIKDMPSACRFLDSYWQGHEEIRQISKSDDAILGWTKGRALVGGHRLFEMLKEGK VNPSPYMKISYEHGGAFLGDILLYDSRREPGSAIFVGNINSMLNNQFSPEYGVQSGVRDR SKRKRPFPGLAWASMKDTYGACPIYSDVLEAIERCWWNAFGESYRAYREDMLKRDTLELS RYVASMARQAGLAELTPIDLEVLADPNKLQYKWTEADVSANIHEVLMHGVSVEKTERFLR SVMPR
- Number of residues
- 665
- Molecular Weight
- 74921.73
- Theoretical pI
- 7.03
- GO Classification
- Functionsmetal ion binding / nucleotide binding / RNA binding / RNA uridylyltransferase activity / RNA-directed RNA polymerase activityProcessestranscription, DNA-templated / transcription, RNA-templated / viral genome replicationComponentsvirion
- General Function
- Rna-dependent RNA polymerase part of the packaging complex that packages the viral RNA segments, replicate them into a double-stranded form and transcribe them.
- Specific Function
- metal ion binding
- Pfam Domain Function
- RdRP_1 (PF00680)
- Signal Regions
- Not Available
- Transmembrane Regions
- Not Available
- Cellular Location
- Virion
- Gene sequence
>lcl|BSEQ0011022|RNA-directed RNA polymerase (P2) ATGCCGAGGAGAGCTCCCGCGTTCCCTCTGAGCGATATCAAGGCTCAGATGCTGTTCGCA AATAACATCAAGGCCCAACAAGCCTCGAAGCGTAGCTTCAAAGAGGGGGCGATTGAAACG TACGAAGGGCTGCTTTCAGTAGACCCTCGGTTTTTGAGTTTCAAGAACGAGCTCTCTCGG TATCTGACCGACCACTTCCCGGCGAACGTCGACGAGTATGGTCGTGTTTATGGAAACGGT GTTCGTACCAACTTCTTTGGTATGCGCCACATGAACGGGTTTCCAATGATCCCCGCGACG TGGCCACTCGCTTCCAACCTTAAGAAACGTGCCGACGCTGACCTAGCCGATGGCCCTGTT TCTGAGCGCGACAATCTACTCTTTCGCGCCGCAGTCCGGCTTATGTTTTCAGATCTAGAG CCTGTTCCGCTGAAGATCCGTAAAGGATCGTCAACCTGCATCCCGTATTTTTCTAACGAT ATGGGAACGAAGATCGAGATCGCCGAGCGCGCTCTTGAGAAAGCGGAAGAAGCTGGCAAT CTGATGCTGCAAGGTAAGTTTGATGACGCCTACCAGCTCCACCAAATGGGTGGTGCCTAT TACGTCGTGTATCGTGCACAATCGACCGATGCTATCACACTCGACCCTAAGACCGGAAAA TTCGTGTCAAAGGATCGTATGGTCGCTGACTTCGAATACGCAGTCACGGGCGGTGAGCAA GGCTCGCTGTTCGCTGCTTCGAAGGATGCCTCTCGTTTGAAGGAACAGTACGGGATAGAT GTCCCGGACGGGTTTTTCTGCGAGCGGCGTCGTACCGCTATGGGTGGTCCGTTCGCGTTG AACGCTCCTATCATGGCCGTTGCGCAACCTGTGCGAAACAAAATTTACTCCAAGTACGCT TACACCTTTCACCATACTACTCGTCTTAATAAGGAGGAAAAGGTGAAAGAGTGGTCGTTG TGCGTCGCTACTGACGTATCCGACCACGACACGTTCTGGCCTGGATGGCTGCGGGATCTC ATCTGTGATGAACTGCTCAACATGGGGTACGCTCCGTGGTGGGTTAAGTTGTTCGAGACC TCGCTCAAACTGCCCGTTTACGTGGGCGCTCCTGCTCCTGAGCAGGGCCACACGTTGTTG GGTGATCCGTCCAACCCTGATCTCGAAGTTGGTCTCTCGTCCGGACAAGGGGCGACCGAC CTCATGGGCACGTTGCTCATGAGTATCACCTACCTGGTGATGCAACTTGATCACACCGCT CCTCACCTCAACAGTCGAATCAAGGACATGCCATCAGCATGCCGCTTTCTTGACTCGTAT TGGCAAGGACACGAGGAGATCCGTCAGATCTCAAAATCTGATGATGCTATACTTGGCTGG ACCAAAGGTCGTGCTTTGGTTGGTGGTCATCGTTTGTTCGAGATGCTGAAAGAGGGTAAG GTTAACCCCTCACCTTACATGAAGATCTCCTACGAGCACGGTGGCGCCTTCCTTGGTGAC ATCCTGCTTTACGACTCGCGTCGTGAGCCTGGCTCTGCCATCTTCGTTGGTAACATCAAC TCAATGCTGAACAACCAGTTCAGCCCTGAGTACGGTGTCCAATCGGGCGTTCGCGACCGA TCTAAGCGCAAACGGCCGTTCCCCGGTCTTGCTTGGGCGTCGATGAAAGATACCTACGGT GCCTGTCCGATCTACTCTGATGTGCTGGAGGCGATCGAGCGTTGCTGGTGGAACGCGTTC GGTGAGTCGTACCGTGCGTATCGTGAAGATATGCTTAAACGCGACACTCTCGAACTATCA CGCTACGTTGCGTCGATGGCTCGTCAAGCCGGGCTGGCTGAACTCACTCCCATTGATTTG GAGGTGCTTGCTGACCCGAACAAACTCCAGTATAAGTGGACCGAGGCCGATGTCTCGGCG AATATCCACGAGGTACTGATGCATGGCGTATCGGTCGAAAAGACTGAGCGCTTTCTCCGT TCTGTAATGCCGAGGTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P11124 UniProtKB Entry Name RDRP_BPPH6 GenBank Protein ID 215481 GenBank Gene ID M17461 PDB ID(s) 1HHS, 1HHT, 1HI0, 1HI1, 1HI8, 1UVI, 1UVJ, 1UVK, 1UVL, 1UVM, 1UVN, 1WAC, 2JL9, 2JLF, 2JLG, 4A8F, 4A8K, 4A8M, 4A8O, 4A8Q, 4A8S, 4A8W, 4A8Y, 4B02, 5FJ6, 5FJ7 KEGG ID vg:956436 NCBI Gene ID 956436 - General References
- Mindich L, Nemhauser I, Gottlieb P, Romantschuk M, Carton J, Frucht S, Strassman J, Bamford DH, Kalkkinen N: Nucleotide sequence of the large double-stranded RNA segment of bacteriophage phi 6: genes specifying the viral replicase and transcriptase. J Virol. 1988 Apr;62(4):1180-5. [Article]
- Katz G, Wei H, Alimova A, Katz A, Morgan DG, Gottlieb P: Protein P7 of the cystovirus phi6 is located at the three-fold axis of the unexpanded procapsid. PLoS One. 2012;7(10):e47489. doi: 10.1371/journal.pone.0047489. Epub 2012 Oct 15. [Article]
- Butcher SJ, Grimes JM, Makeyev EV, Bamford DH, Stuart DI: A mechanism for initiating RNA-dependent RNA polymerization. Nature. 2001 Mar 8;410(6825):235-40. [Article]
- Salgado PS, Makeyev EV, Butcher SJ, Bamford DH, Stuart DI, Grimes JM: The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase. Structure. 2004 Feb;12(2):307-16. [Article]
- Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH: Back-priming mode of phi6 RNA-dependent RNA polymerase. J Gen Virol. 2005 Feb;86(Pt 2):521-6. [Article]
Associated Data
- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details Guanosine-5'-Triphosphate experimental unknown target Details