47 kDa membrane antigen
Details
- Name
- 47 kDa membrane antigen
- Kind
- protein
- Synonyms
- 47 kDa membrane antigen precursor
- Gene Name
- Not Available
- UniProtKB Entry
- P29723Swiss-Prot
- Organism
- Treponema pallidum (strain Nichols)
- NCBI Taxonomy ID
- 243276
- Amino acid sequence
>lcl|BSEQ0011358|47 kDa membrane antigen MKVKYALLSAGALQLLVVGCGSSHHETHYGYATLSYADYWAGELGQSRDVLLAGNAEADR AGDLDAGMFDAVSRATHGHGAFRQQFQYAVEVLGEKVLSKQETEDSRGRKKWEYETDPSV TKMVRASASFQDLGEDGEIKFEAVEGAVALADRASSFMVDSEEYKITNVKVHGMKFVPVA VPHELKGIAKEKFHFVEDSRVTENTNGLKTMLTEDSFSARKVSSMESPHDLVVDTVGTGY HSRFGSDAEASVMLKRADGSELSHREFIDYVMNFNTVRYDYYGDDASYTNLMASYGTKHS ADSWWKTGRVPRISCGINYGFDRFKGSGPGYYRLTLIANGYRDVVADVRFLPKYEGNIDI GLKGKVLTIGGADAETLMDAAVDVFADGQPKLVSDQAVSLGQNVLSADFTPGTEYTVEVR FKEFGSVRAKVVAQ
- Number of residues
- 434
- Molecular Weight
- 47664.955
- Theoretical pI
- 5.34
- GO Classification
- Componentsplasma membrane
- General Function
- A possible D,D-carboxypeptidase, that releases amino acids sequentially from a proteins C-terminus (PubMed:12196546, PubMed:7972112). Has zinc-dependent carboxypeptidase activity on synthetic depsipeptide substrates (PubMed:7972112). May serve to decrease cross-linking of peptidoglycan, promoting the highly sinusous motility of this spirochaete (Probable). Overexpression of the whole protein in E.coli leads to aberrant cell morphology and extrusion of the cytoplasm, while overexpression of a construct with the first 62 resides of the protein fused to PhoA does have this effect, suggesting the whole protein, not the lipoprotein moiety, is toxic (PubMed:7972112). Binds penicillin (PubMed:2647634, PubMed:7972112). Penicillin binding is covalent, does not require lipidation, and is zinc-dependent (PubMed:12196546, PubMed:7972112). While this protein has beta-lactamase activity in vitro, that is probably not its role in vivo, as T.pallidum is very sensitive to penicillin antibiotics (PubMed:12196546).
- Specific Function
- peptidase activity
- Pfam Domain Function
- Signal Regions
- 1-19
- Transmembrane Regions
- Not Available
- Cellular Location
- Cell membrane
- Gene sequence
>lcl|BSEQ0011359|47 kDa membrane antigen GTGAAAGTGAAATACGCACTACTTTCTGCCGGAGCGCTGCAGTTGTTGGTTGTAGGCTGT GGCTCGTCTCATCATGAGACGCACTATGGCTATGCGACGCTAAGCTATGCGGACTACTGG GCCGGGGAGTTGGGGCAGAGTAGGGACGTGCTTTTGGCGGGTAATGCCGAGGCGGACCGC GCGGGGGATCTCGACGCAGGCATGTTCGATGCAGTTTCTCGCGCAACCCACGGGCATGGC GCGTTCCGTCAGCAATTTCAGTACGCGGTTGAGGTATTGGGCGAAAAGGTTCTCTCGAAG CAGGAGACCGAAGACAGCAGGGGAAGAAAAAAGTGGGAGTACGAGACTGACCCAAGCGTT ACTAAGATGGTGCGTGCCTCTGCGTCATTTCAGGATTTGGGAGAGGACGGGGAGATTAAG TTTGAAGCAGTCGAGGGTGCAGTAGCGTTGGCGGATCGCGCGAGTTCCTTCATGGTTGAC AGCGAGGAATACAAGATTACGAACGTAAAGGTTCACGGTATGAAGTTTGTCCCAGTTGCG GTTCCTCATGAATTAAAAGGGATTGCAAAGGAGAAGTTTCACTTCGTGGAAGACTCCCGC GTTACGGAGAATACCAACGGCCTTAAGACAATGCTCACTGAGGATAGTTTTTCTGCACGT AAGGTAAGCAGCATGGAGAGCCCGCACGACCTTGTGGTAGACACGGTGGGTACCGGTTAC CACAGCCGTTTTGGTTCGGACGCAGAGGCTTCTGTGATGCTGAAAAGGGCTGATGGCTCT GAGCTGTCGCACCGTGAGTTCATCGACTATGTGATGAACTTCAACACGGTCCGCTACGAC TACTACGGTGATGACGCGAGCTACACCAATCTGATGGCGAGTTATGGCACCAAGCACTCT GCTGACTCCTGGTGGAAGACAGGAAGAGTGCCCCGCATTTCGTGTGGTATCAACTATGGG TTCGATCGGTTTAAAGGTTCAGGGCCGGGATACTACAGGCTGACTTTGATTGCGAACGGG TATAGGGACGTAGTTGCTGATGTGCGCTTCCTTCCCAAGTACGAGGGGAACATCGATATT GGGTTGAAGGGGAAGGTGCTGACCATAGGGGGCGCGGACGCGGAGACTCTGATGGATGCT GCAGTTGACGTGTTTGCCGATGGACAGCCTAAGCTTGTCAGCGATCAAGCGGTGAGCTTG GGGCAGAATGTCCTCTCTGCGGATTTCACTCCCGGCACTGAGTACACGGTTGAGGTTAGG TTCAAGGAATTCGGTTCTGTGCGTGCGAAGGTAGTGGCCCAGTAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P29723 UniProtKB Entry Name TA47_TREPA GenBank Protein ID 155053 GenBank Gene ID M88769 PDB ID(s) 1O75 KEGG ID tpa:TP_0574 NCBI Gene ID 2611716 - General References
- Weigel LM, Brandt ME, Norgard MV: Analysis of the N-terminal region of the 47-kilodalton integral membrane lipoprotein of Treponema pallidum. Infect Immun. 1992 Apr;60(4):1568-76. [Article]
- Fraser CM, Norris SJ, Weinstock GM, White O, Sutton GG, Dodson R, Gwinn M, Hickey EK, Clayton R, Ketchum KA, Sodergren E, Hardham JM, McLeod MP, Salzberg S, Peterson J, Khalak H, Richardson D, Howell JK, Chidambaram M, Utterback T, McDonald L, Artiach P, Bowman C, Cotton MD, Fujii C, Garland S, Hatch B, Horst K, Roberts K, Sandusky M, Weidman J, Smith HO, Venter JC: Complete genome sequence of Treponema pallidum, the syphilis spirochete. Science. 1998 Jul 17;281(5375):375-88. [Article]
- Hsu PL, Chamberlain NR, Orth K, Moomaw CR, Zhang LQ, Slaughter CA, Radolf JD, Sell S, Norgard MV: Sequence analysis of the 47-kilodalton major integral membrane immunogen of Treponema pallidum. Infect Immun. 1989 Jan;57(1):196-203. [Article]
Associated Data
- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details 2,3-Di-O-Sulfo-Alpha-D-Glucopyranose experimental unknown target Details