Outer membrane protein TolC
Details
- Name
- Outer membrane protein TolC
- Kind
- protein
- Synonyms
- colE1-i
- mtcB
- mukA
- Multidrug efflux pump subunit TolC
- Outer membrane factor TolC
- refI
- toc
- weeA
- Gene Name
- tolC
- UniProtKB Entry
- P02930Swiss-Prot
- Organism
- Escherichia coli (strain K12)
- NCBI Taxonomy ID
- 83333
- Amino acid sequence
>lcl|BSEQ0003825|Outer membrane protein TolC MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAADRDAAFEKINEARSPL LPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSKWRALTLQEKAAGIQDVTY QTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLDQTTQRFNVGLVAITDVQNAR AQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAALNVENFKTDKPQPVNALLKEAEK RNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASTGISDTSYSGSKTRGAAGTQYDDSN MGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGASEQLESAHRSVVQTVRSSFNNINASI SSINAYKQAVVSAQSSLDAMEAGYSVGTRTIVDVLDATTTLYNAKQELANARYNYLINQL NIKSALGTLNEQDLLALNNALSKPVSTNPENVAPQTPEQNAIADGYAPDSPAPVVQQTSA RTTTSNGHNPFRN
- Number of residues
- 493
- Molecular Weight
- 53740.24
- Theoretical pI
- 5.3
- GO Classification
- Functionsefflux transmembrane transporter activity / identical protein binding / porin activityProcessesenterobactin transport / response to antibiotic / response to organic cyclic compound / transmembrane transportComponentscell outer membrane / integral component of membrane
- General Function
- Outer membrane channel, which is required for the function of several efflux systems such as AcrAB-TolC, AcrEF-TolC, EmrAB-TolC and MacAB-TolC. These systems are involved in export of antibiotics and other toxic compounds from the cell. TolC is also involved in import of colicin E1 into the cells.
- Specific Function
- bile acid transmembrane transporter activity
- Pfam Domain Function
- OEP (PF02321)
- Signal Regions
- 1-22
- Transmembrane Regions
- 63-74 83-96 269-279 301-311
- Cellular Location
- Cell outer membrane
- Gene sequence
>lcl|BSEQ0019205|Outer membrane protein TolC (tolC) ATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGC CAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGT AAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGTCCATTA CTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAAC GGCATCAACTCTAACGCGACCAGTGCGTCCTTGCAGTTAACTCAATCCATTTTTGATATG TCGAAATGGCGTGCGTTAACGCTGCAGGAAAAAGCAGCAGGGATTCAGGACGTCACGTAT CAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTGAAT GCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGAT CAAACCACCCAACGTTTTAACGTGGGCCTGGTAGCGATCACCGACGTGCAGAACGCCCGC GCACAGTACGATACCGTGCTGGCGAACGAAGTGACCGCACGTAATAACCTTGATAACGCG GTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTC GAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAA CGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGC CAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGAC ACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAAT ATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTT AACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGT GCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATC AGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGACGCGATG GAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACG TTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTG AATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCG CTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAAT GCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCA CGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P02930 UniProtKB Entry Name TOLC_ECOLI GenBank Protein ID 43107 GenBank Gene ID X54049 PDB ID(s) 1EK9, 1TQQ, 2VDD, 2VDE, 2WMZ, 2XMN KEGG ID ecj:JW5503 NCBI Gene ID 947521 - General References
- Hackett J, Reeves P: Primary structure of the tolC gene that codes for an outer membrane protein of Escherichia coli K12. Nucleic Acids Res. 1983 Sep 24;11(18):6487-95. [Article]
- Niki H, Imamura R, Ogura T, Hiraga S: Nucleotide sequence of the tolC gene of Escherichia coli. Nucleic Acids Res. 1990 Sep 25;18(18):5547. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Hackett J, Misra R, Reeves P: The TolC protein of Escherichia coli K12 is synthesised in a precursor form. FEBS Lett. 1983 Jun 13;156(2):307-10. [Article]
- Morona R, Manning PA, Reeves P: Identification and characterization of the TolC protein, an outer membrane protein from Escherichia coli. J Bacteriol. 1983 Feb;153(2):693-9. [Article]
- Link AJ, Robison K, Church GM: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis. 1997 Aug;18(8):1259-313. [Article]
- Molloy MP, Herbert BR, Walsh BJ, Tyler MI, Traini M, Sanchez JC, Hochstrasser DF, Williams KL, Gooley AA: Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis. 1998 May;19(5):837-44. [Article]
- Koronakis V, Li J, Koronakis E, Stauffer K: Structure of TolC, the outer membrane component of the bacterial type I efflux system, derived from two-dimensional crystals. Mol Microbiol. 1997 Feb;23(3):617-26. [Article]
- Kobayashi K, Tsukagoshi N, Aono R: Suppression of hypersensitivity of Escherichia coli acrB mutant to organic solvents by integrational activation of the acrEF operon with the IS1 or IS2 element. J Bacteriol. 2001 Apr;183(8):2646-53. [Article]
- Touze T, Eswaran J, Bokma E, Koronakis E, Hughes C, Koronakis V: Interactions underlying assembly of the Escherichia coli AcrAB-TolC multidrug efflux system. Mol Microbiol. 2004 Jul;53(2):697-706. [Article]
- Stenberg F, Chovanec P, Maslen SL, Robinson CV, Ilag LL, von Heijne G, Daley DO: Protein complexes of the Escherichia coli cell envelope. J Biol Chem. 2005 Oct 14;280(41):34409-19. Epub 2005 Aug 3. [Article]
- Lin HT, Bavro VN, Barrera NP, Frankish HM, Velamakanni S, van Veen HW, Robinson CV, Borges-Walmsley MI, Walmsley AR: MacB ABC transporter is a dimer whose ATPase activity and macrolide-binding capacity are regulated by the membrane fusion protein MacA. J Biol Chem. 2009 Jan 9;284(2):1145-54. doi: 10.1074/jbc.M806964200. Epub 2008 Oct 27. [Article]
- Symmons MF, Bokma E, Koronakis E, Hughes C, Koronakis V: The assembled structure of a complete tripartite bacterial multidrug efflux pump. Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7173-8. doi: 10.1073/pnas.0900693106. Epub 2009 Apr 2. [Article]
- Tikhonova EB, Dastidar V, Rybenkov VV, Zgurskaya HI: Kinetic control of TolC recruitment by multidrug efflux complexes. Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16416-21. doi: 10.1073/pnas.0906601106. Epub 2009 Sep 2. [Article]
- Xu Y, Song S, Moeller A, Kim N, Piao S, Sim SH, Kang M, Yu W, Cho HS, Chang I, Lee K, Ha NC: Functional implications of an intermeshing cogwheel-like interaction between TolC and MacA in the action of macrolide-specific efflux pump MacAB-TolC. J Biol Chem. 2011 Apr 15;286(15):13541-9. doi: 10.1074/jbc.M110.202598. Epub 2011 Feb 16. [Article]
- Fontaine F, Fuchs RT, Storz G: Membrane localization of small proteins in Escherichia coli. J Biol Chem. 2011 Sep 16;286(37):32464-74. doi: 10.1074/jbc.M111.245696. Epub 2011 Jul 21. [Article]
- Zakharov SD, Sharma O, Zhalnina M, Yamashita E, Cramer WA: Pathways of colicin import: utilization of BtuB, OmpF porin and the TolC drug-export protein. Biochem Soc Trans. 2012 Dec 1;40(6):1463-8. doi: 10.1042/BST20120211. [Article]
- Koronakis V, Sharff A, Koronakis E, Luisi B, Hughes C: Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export. Nature. 2000 Jun 22;405(6789):914-9. [Article]
- Higgins MK, Eswaran J, Edwards P, Schertler GF, Hughes C, Koronakis V: Structure of the ligand-blocked periplasmic entrance of the bacterial multidrug efflux protein TolC. J Mol Biol. 2004 Sep 17;342(3):697-702. [Article]
- Bavro VN, Pietras Z, Furnham N, Perez-Cano L, Fernandez-Recio J, Pei XY, Misra R, Luisi B: Assembly and channel opening in a bacterial drug efflux machine. Mol Cell. 2008 Apr 11;30(1):114-21. doi: 10.1016/j.molcel.2008.02.015. [Article]
- Pei XY, Hinchliffe P, Symmons MF, Koronakis E, Benz R, Hughes C, Koronakis V: Structures of sequential open states in a symmetrical opening transition of the TolC exit duct. Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):2112-7. doi: 10.1073/pnas.1012588108. Epub 2011 Jan 18. [Article]
Associated Data
- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details Cobalt hexammine ion experimental unknown target Details