Fatty-acid peroxygenase

Details

Name
Fatty-acid peroxygenase
Kind
protein
Synonyms
  • 1.11.2.4
  • CYP152A1
  • Cytochrome P450 152A1
  • Cytochrome P450BsBeta
  • Fatty acid beta-hydroxylase
Gene Name
cypC
UniProtKB Entry
O31440Swiss-Prot
Organism
Bacillus subtilis (strain 168)
NCBI Taxonomy ID
224308
Amino acid sequence
>lcl|BSEQ0011411|Fatty-acid peroxygenase
MNEQIPHDKSLDNSLTLLKEGYLFIKNRTERYNSDLFQARLLGKNFICMTGAEAAKVFYD
TDRFQRQNALPKRVQKSLFGVNAIQGMDGSAHIHRKMLFLSLMTPPHQKRLAELMTEEWK
AAVTRWEKADEVVLFEEAKEILCRVACYWAGVPLKETEVKERADDFIDMVDAFGAVGPRH
WKGRRARPRAEEWIEVMIEDARAGLLKTTSGTALHEMAFHTQEDGSQLDSRMAAIELINV
LRPIVAISYFLVFSALALHEHPKYKEWLRSGNSREREMFVQEVRRYYPFGPFLGALVKKD
FVWNNCEFKKGTSVLLDLYGTNHDPRLWDHPDEFRPERFAEREENLFDMIPQGGGHAEKG
HRCPGEGITIEVMKASLDFLVHQIEYDVPEQSLHYSLARMPSLPESGFVMSGIRRKS
Number of residues
417
Molecular Weight
48108.7
Theoretical pI
6.93
GO Classification
Functions
heme binding / iron ion binding / monooxygenase activity / oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
General Function
Catalyzes the alpha- and beta-hydroxylation of myristic acid in the presence of hydrogen peroxide.
Specific Function
heme binding
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0011412|Fatty-acid peroxygenase (cypC)
ATGAATGAGCAGATTCCACATGACAAAAGTCTCGATAACAGTCTGACACTGCTGAAGGAA
GGGTATTTATTTATTAAAAACAGAACAGAGCGCTACAATTCAGATCTGTTTCAGGCCCGT
TTGTTGGGAAAAAACTTTATTTGCATGACTGGCGCTGAGGCGGCGAAGGTGTTTTATGAT
ACGGATCGATTCCAGCGGCAGAACGCTTTGCCTAAGCGGGTGCAGAAATCGCTGTTTGGT
GTTAATGCGATTCAGGGAATGGATGGCAGCGCGCATATCCATCGGAAGATGCTTTTTCTG
TCATTGATGACACCGCCGCATCAAAAACGTTTGGCTGAGTTGATGACAGAGGAGTGGAAA
GCAGCAGTCACAAGATGGGAGAAGGCAGATGAGGTTGTGTTATTTGAAGAAGCAAAAGAA
ATCCTGTGCCGGGTAGCGTGCTATTGGGCAGGTGTTCCGTTGAAGGAAACGGAAGTCAAA
GAGAGAGCGGATGACTTCATTGACATGGTCGACGCGTTCGGTGCTGTGGGACCGCGGCAT
TGGAAAGGAAGAAGAGCAAGGCCGCGTGCGGAAGAGTGGATTGAAGTCATGATTGAAGAT
GCTCGTGCCGGCTTGCTGAAAACGACTTCCGGAACAGCGCTGCATGAAATGGCTTTTCAC
ACACAAGAAGATGGAAGCCAGCTGGATTCCCGCATGGCAGCCATTGAGCTGATTAATGTA
CTGCGGCCTATTGTCGCCATTTCTTACTTTCTGGTGTTTTCAGCTTTGGCGCTTCATGAG
CATCCGAAGTATAAGGAATGGCTGCGGTCTGGAAACAGCCGGGAAAGAGAAATGTTTGTG
CAGGAGGTCCGCAGATATTATCCGTTCGGCCCGTTTTTAGGGGCGCTTGTCAAAAAAGAT
TTTGTATGGAATAACTGTGAGTTTAAGAAGGGCACATCGGTGCTGCTTGATTTATATGGA
ACGAACCACGACCCTCGTCTATGGGATCATCCCGATGAATTCCGGCCGGAACGATTTGCG
GAGCGGGAAGAAAATCTGTTTGATATGATTCCTCAAGGCGGGGGGCACGCCGAGAAAGGC
CACCGCTGTCCAGGGGAAGGCATTACAATTGAAGTCATGAAAGCGAGCCTGGATTTCCTC
GTCCATCAGATTGAATACGATGTTCCGGAACAATCACTGCATTACAGTCTCGCCAGAATG
CCATCATTGCCTGAAAGCGGCTTCGTAATGAGCGGAATCAGACGAAAAAGTTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDO31440
UniProtKB Entry NameCYPC_BACSU
GenBank Protein ID3599632
GenBank Gene IDAB006424
PDB ID(s)1IZO, 2ZQJ, 2ZQX
KEGG IDbsu:BSU02100
NCBI Gene ID938449
General References
  1. Matsunaga I, Ueda A, Fujiwara N, Sumimoto T, Ichihara K: Characterization of the ybdT gene product of Bacillus subtilis: novel fatty acid beta-hydroxylating cytochrome P450. Lipids. 1999 Aug;34(8):841-6. [Article]
  2. Kunst F, Ogasawara N, Moszer I, Albertini AM, Alloni G, Azevedo V, Bertero MG, Bessieres P, Bolotin A, Borchert S, Borriss R, Boursier L, Brans A, Braun M, Brignell SC, Bron S, Brouillet S, Bruschi CV, Caldwell B, Capuano V, Carter NM, Choi SK, Cordani JJ, Connerton IF, Cummings NJ, Daniel RA, Denziot F, Devine KM, Dusterhoft A, Ehrlich SD, Emmerson PT, Entian KD, Errington J, Fabret C, Ferrari E, Foulger D, Fritz C, Fujita M, Fujita Y, Fuma S, Galizzi A, Galleron N, Ghim SY, Glaser P, Goffeau A, Golightly EJ, Grandi G, Guiseppi G, Guy BJ, Haga K, Haiech J, Harwood CR, Henaut A, Hilbert H, Holsappel S, Hosono S, Hullo MF, Itaya M, Jones L, Joris B, Karamata D, Kasahara Y, Klaerr-Blanchard M, Klein C, Kobayashi Y, Koetter P, Koningstein G, Krogh S, Kumano M, Kurita K, Lapidus A, Lardinois S, Lauber J, Lazarevic V, Lee SM, Levine A, Liu H, Masuda S, Mauel C, Medigue C, Medina N, Mellado RP, Mizuno M, Moestl D, Nakai S, Noback M, Noone D, O'Reilly M, Ogawa K, Ogiwara A, Oudega B, Park SH, Parro V, Pohl TM, Portelle D, Porwollik S, Prescott AM, Presecan E, Pujic P, Purnelle B, Rapoport G, Rey M, Reynolds S, Rieger M, Rivolta C, Rocha E, Roche B, Rose M, Sadaie Y, Sato T, Scanlan E, Schleich S, Schroeter R, Scoffone F, Sekiguchi J, Sekowska A, Seror SJ, Serror P, Shin BS, Soldo B, Sorokin A, Tacconi E, Takagi T, Takahashi H, Takemaru K, Takeuchi M, Tamakoshi A, Tanaka T, Terpstra P, Togoni A, Tosato V, Uchiyama S, Vandebol M, Vannier F, Vassarotti A, Viari A, Wambutt R, Wedler H, Weitzenegger T, Winters P, Wipat A, Yamamoto H, Yamane K, Yasumoto K, Yata K, Yoshida K, Yoshikawa HF, Zumstein E, Yoshikawa H, Danchin A: The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature. 1997 Nov 20;390(6657):249-56. [Article]
  3. Lee DS, Yamada A, Sugimoto H, Matsunaga I, Ogura H, Ichihara K, Adachi S, Park SY, Shiro Y: Substrate recognition and molecular mechanism of fatty acid hydroxylation by cytochrome P450 from Bacillus subtilis. Crystallographic, spectroscopic, and mutational studies. J Biol Chem. 2003 Mar 14;278(11):9761-7. Epub 2003 Jan 7. [Article]

Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
Palmitoleic AcidexperimentalunknowntargetDetails