Alpha-lytic protease
Details
- Name
- Alpha-lytic protease
- Kind
- protein
- Synonyms
- 3.4.21.12
- Alpha-lytic endopeptidase
- Gene Name
- alpha-LP
- UniProtKB Entry
- P00778Swiss-Prot
- Organism
- Lysobacter enzymogenes
- NCBI Taxonomy ID
- 69
- Amino acid sequence
>lcl|BSEQ0011418|Alpha-lytic protease MYVSNHRSRRVARVSVSCLVAALAAMSCGAALAADQVDPQLKFAMQRDLGIFPTQLPQYL QTEKLARTQAAAIEREFGAQFAGSWIERNEDGSFKLVAATSGARKSSTLGGVEVRNVRYS LKQLQSAMEQLDAGANARVKGVSKPLDGVQSWYVDPRSNAVVVKVDDGATEAGVDFVALS GADSAQVRIESSPGKLQTTANIVGGIEYSINNASLCSVGFSVTRGATKGFVTAGHCGTVN ATARIGGAVVGTFAARVFPGNDRAWVSLTSAQTLLPRVANGSSFVTVRGSTEAAVGAAVC RSGRTTGYQCGTITAKNVTANYAEGAVRGLTQGNACMGRGDSGGSWITSAGQAQGVMSGG NVQSNGNNCGIPASQRSSLFERLQPILSQYGLSLVTG
- Number of residues
- 397
- Molecular Weight
- 41076.9
- Theoretical pI
- 9.61
- GO Classification
- Functionsserine-type endopeptidase activityComponentsextracellular region
- General Function
- Not Available
- Specific Function
- serine-type endopeptidase activity
- Pfam Domain Function
- Signal Regions
- 1-24
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0003898|1194 bp ATGTACGTATCGAACCACCGTTCGCGCCGCGTCGCGCGCGTGTCCGTTTCCTGCCTCGTC GCCGCGCTGGCGGCGATGTCCTGCGGCGCTGCGCTGGCGGCCGATCAGGTCGATCCTCAG CTGAAATTCGCCATGCAGCGCGATCTGGGCATCTTCCCGACCCAGCTGCCGCAGTACCTG CAGACCGAAAAACTCGCCCGCACCCAGGCCGCGGCGATCGAGCGCGAGTTCGGTGCCCAG TTCGCCGGCAGCTGGATCGAGCGCAACGAAGACGGCAGCTTCAAGCTCGTCGCCGCGACC TCGGGCGCGCGCAAGTCGAGCACGCTGGGCGGGGTGGAAGTGCGCAACGTGCGCTACAGC CTCAAGCAGCTGCAGTCGGCGATGGAGCAGCTCGACGCCGGCGCCAACGCGCGGGTGAAG GGCGTCAGCAAGCCGCTCGACGGCGTGCAGAGCTGGTACGTGGATCCGCGCAGCAACGCG GTGGTGGTCAAAGTCGACGACGGCGCGACGGAAGCCGGCGTCGACTTCGTCGCCCTCAGC GGCGCCGACAGCGCGCAGGTGCGGATCGAAAGCTCGCCGGGCAAGCTGCAGACCACGGCC AACATCGTCGGCGGCATCGAGTACTCGATCAACAACGCCTCGCTGTGCTCGGTCGGCTTC TCGGTCACCCGCGGCGCGACCAAGGGCTTCGTCACCGCCGGCCACTGCGGCACGGTGAAT GCGACCGCGCGCATCGGCGGCGCGGTGGTCGGCACCTTCGCCGCGCGCGTGTTCCCCGGC AACGACCGCGCCTGGGTCAGCCTGACCAGCGCGCAGACCCTGCTGCCGCGCGTGGCCAAC GGCAGCAGCTTCGTCACCGTGCGCGGCAGCACCGAGGCCGCGGTCGGCGCGGCGGTGTGC CGCTCGGGCCGCACCACCGGTTACCAGTGCGGCACCATCACCGCCAAGAACGTCACCGCC AACTACGCCGAAGGTGCGGTGCGCGGCCTGACCCAGGGCAACGCCTGTATGGGCCGCGGC GATTCGGGCGGTTCGTGGATCACCAGCGCCGGCCAGGCGCAGGGCGTGATGTCGGGCGGC AACGTGCAGTCCAACGGCAACAACTGCGGCATCCCGGCCTCGCAGCGCAGCAGCCTGTTC GAGCGTCTGCAGCCGATCCTGAGCCAGTACGGCCTGAGCCTGGTCACCGGCTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P00778 UniProtKB Entry Name PRLA_LYSEN GenBank Protein ID 150130 GenBank Gene ID J04052 PDB ID(s) 1BOQ, 1GBA, 1GBB, 1GBC, 1GBD, 1GBE, 1GBF, 1GBH, 1GBI, 1GBJ, 1GBK, 1GBL, 1GBM, 1P01, 1P02, 1P03, 1P04, 1P05, 1P06, 1P09, 1P10, 1P11, 1P12, 1QQ4, 1QRW, 1QRX, 1SSX, 1TAL, 2ALP, 2H5C, 2H5D, 2LPR, 2PRO, 2ULL, 3LPR, 3M7T, 3M7U, 3PRO, 3QGJ, 3URC, 3URD, 3URE, 4PRO, 5LPR, 6LPR, 7LPR, 8LPR, 9LPR - General References
- Epstein DM, Wensink PC: The alpha-lytic protease gene of Lysobacter enzymogenes. The nucleotide sequence predicts a large prepro-peptide with homology to pro-peptides of other chymotrypsin-like enzymes. J Biol Chem. 1988 Nov 15;263(32):16586-90. [Article]
- Silen JL, McGrath CN, Smith KR, Agard DA: Molecular analysis of the gene encoding alpha-lytic protease: evidence for a preproenzyme. Gene. 1988 Sep 30;69(2):237-44. [Article]
- Olson MO, Nagabhushan N, Dzwiniel M, Smillie LB, Whitaker DR: Primary structure of alpha-lytic protease: a bacterial homologue of the pancreatic serine proteases. Nature. 1970 Oct 31;228(5270):438-42. [Article]
- Brayer GD, Delbaere LT, James MN: Molecular structure of the alpha-lytic protease from Myxobacter 495 at 2.8 Angstroms resolution. J Mol Biol. 1979 Jul 15;131(4):743-75. [Article]
- Fujinaga M, Delbaere LT, Brayer GD, James MN: Refined structure of alpha-lytic protease at 1.7 A resolution. Analysis of hydrogen bonding and solvent structure. J Mol Biol. 1985 Aug 5;184(3):479-502. [Article]
- Peters RJ, Shiau AK, Sohl JL, Anderson DE, Tang G, Silen JL, Agard DA: Pro region C-terminus:protease active site interactions are critical in catalyzing the folding of alpha-lytic protease. Biochemistry. 1998 Sep 1;37(35):12058-67. [Article]
- Sauter NK, Mau T, Rader SD, Agard DA: Structure of alpha-lytic protease complexed with its pro region. Nat Struct Biol. 1998 Nov;5(11):945-50. [Article]
Associated Data
- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details Tris(Hydroxyethyl)Aminomethane experimental unknown target Details PHENYLALANINE BORONIC ACID experimental unknown target Details