Arabinose operon regulatory protein

Details

Name
Arabinose operon regulatory protein
Kind
protein
Synonyms
Not Available
Gene Name
araC
UniProtKB Entry
P0A9E0Swiss-Prot
Organism
Escherichia coli (strain K12)
NCBI Taxonomy ID
83333
Amino acid sequence
>lcl|BSEQ0016657|Arabinose operon regulatory protein
MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQ
GREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFF
RPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVR
EACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLS
TTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS
Number of residues
292
Molecular Weight
33383.625
Theoretical pI
6.95
GO Classification
Functions
core promoter proximal region sequence-specific DNA binding / transcription factor activity, sequence-specific DNA binding
Processes
arabinose catabolic process / regulation of transcription, DNA-templated / transcription, DNA-templated
Components
cytosol
General Function
Transcription factor that regulates the expression of several genes involved in the transport and metabolism of L-arabinose (PubMed:1447222, PubMed:2231717, PubMed:2962192, PubMed:328165, PubMed:4362626, PubMed:6251457, PubMed:6319708). Functions both as a positive and a negative regulator (PubMed:328165, PubMed:6251457). In the presence of arabinose, activates the expression of the araBAD, araE, araFGH and araJ promoters (PubMed:1447222, PubMed:2231717, PubMed:2962192, PubMed:328165, PubMed:4362626, PubMed:6251457, PubMed:6319708). In the absence of arabinose, negatively regulates the araBAD operon (PubMed:6251457). Represses its own transcription (PubMed:328165). Acts by binding directly to DNA (PubMed:1447222, PubMed:2531226, PubMed:2962192, PubMed:4943786, PubMed:6251457).
Specific Function
DNA-binding transcription repressor activity
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Cytoplasm
Gene sequence
>lcl|BSEQ0016658|Arabinose operon regulatory protein (araC)
ATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTG
GCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTG
GGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAG
GGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCAT
CACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCG
CGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTT
CGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCC
GGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTA
CTGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGC
GAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTC
GCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGG
ATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGC
ACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAT
TTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGT
GAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP0A9E0
UniProtKB Entry NameARAC_ECOLI
GenBank Protein ID40936
GenBank Gene IDV00256
PDB ID(s)1XJA, 2AAC, 2ARA, 2ARC, 2K9S
KEGG IDecj:JW0063
NCBI Gene ID944780
General References
  1. Wallace RG, Lee N, Fowler AV: The araC gene of Escherichia coli: transcriptional and translational start-points and complete nucleotide sequence. Gene. 1980 Dec;12(3-4):179-90. [Article]
  2. Miyada CG, Horwitz AH, Cass LG, Timko J, Wilcox G: DNA sequence of the araC regulatory gene from Escherichia coli B/r. Nucleic Acids Res. 1980 Nov 25;8(22):5267-74. [Article]
  3. Stoner CM, Schleif R: Is the amino acid but not the nucleotide sequence of the Escherichia coli araC gene conserved? J Mol Biol. 1982 Feb 5;154(4):649-52. [Article]
  4. Yura T, Mori H, Nagai H, Nagata T, Ishihama A, Fujita N, Isono K, Mizobuchi K, Nakata A: Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region. Nucleic Acids Res. 1992 Jul 11;20(13):3305-8. [Article]
  5. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
  6. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
  7. Cass LG, Horwitz AH, Miyada CG, Greenfield L, Wilcox G: The araC regulatory gene mRNA contains a leader sequence. Mol Gen Genet. 1980;180(1):219-26. [Article]
  8. VanBogelen RA, Abshire KZ, Moldover B, Olson ER, Neidhardt FC: Escherichia coli proteome analysis using the gene-protein database. Electrophoresis. 1997 Aug;18(8):1243-51. [Article]
  9. Soisson SM, MacDougall-Shackleton B, Schleif R, Wolberger C: The 1.6 A crystal structure of the AraC sugar-binding and dimerization domain complexed with D-fucose. J Mol Biol. 1997 Oct 17;273(1):226-37. [Article]
  10. Soisson SM, MacDougall-Shackleton B, Schleif R, Wolberger C: Structural basis for ligand-regulated oligomerization of AraC. Science. 1997 Apr 18;276(5311):421-5. [Article]

Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
Alpha-L-ArabinoseexperimentalunknowntargetDetails
beta-D-fucoseexperimentalunknowntargetDetails