Phosphoethanolamine/phosphocholine phosphatase

Details

Name
Phosphoethanolamine/phosphocholine phosphatase
Kind
protein
Synonyms
  • 3.1.3.75
Gene Name
PHOSPHO1
UniProtKB Entry
Q8TCT1Swiss-Prot
Organism
Humans
NCBI Taxonomy ID
9606
Amino acid sequence
>lcl|BSEQ0004289|Phosphoethanolamine/phosphocholine phosphatase
MSGCFPVSGLRCLSRDGRMAAQGAPRFLLTFDFDETIVDENSDDSIVRAAPGQRLPESLR
ATYREGFYNEYMQRVFKYLGEQGVRPRDLSAIYEAIPLSPGMSDLLQFVAKQGACFEVIL
ISDANTFGVESSLRAAGHHSLFRRILSNPSGPDARGLLALRPFHTHSCARCPANMCKHKV
LSDYLRERAHDGVHFERLFYVGDGANDFCPMGLLAGGDVAFPRRGYPMHRLIQEAQKAEP
SSFRASVVPWETAADVRLHLQQVLKSC
Number of residues
267
Molecular Weight
29712.54
Theoretical pI
7.78
GO Classification
Processes
bone mineralization
Components
extracellular matrix / extracellular membrane-bounded organelle
General Function
Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho) (PubMed:15175005). Involved in the generation of inorganic phosphate for bone mineralization (By similarity). Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix (By similarity)
Specific Function
Metal ion binding
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Extracellular vesicle
Gene sequence
>lcl|BSEQ0020598|Phosphoethanolamine/phosphocholine phosphatase (PHOSPHO1)
ATGTGCCAGCGCCTCTGGCCGTGGCCCGCTAACCAGCCTCTCCCGGGCGGGCTCCTGCCG
CGCCCCCTCTCGCTTGCCCCCTCCTCCTCCTCCTCCTGCTGCTCTCCCCCCTGCTCCCAG
GACGGCAGGATGGCCGCGCAGGGCGCGCCGCGCTTCCTCCTGACCTTCGACTTCGACGAG
ACTATCGTGGACGAAAACAGCGACGATTCGATCGTGCGCGCCGCGCCGGGCCAGCGGCTC
CCGGAGAGCCTGCGAGCCACCTACCGCGAGGGCTTCTACAACGAGTACATGCAGCGCGTC
TTCAAGTACCTGGGCGAGCAGGGCGTGCGGCCGCGGGACCTGAGCGCCATCTACGAAGCC
ATCCCTTTGTCGCCAGGCATGAGCGACCTGCTGCAGTTTGTGGCAAAACAGGGCGCCTGC
TTCGAGGTGATTCTCATCTCCGATGCCAACACCTTTGGCGTGGAGAGCTCGCTGCGCGCC
GCCGGCCACCACAGCCTGTTCCGCCGCATCCTCAGCAACCCGTCGGGGCCGGATGCGCGG
GGACTGCTGGCTCTGCGGCCGTTCCACACACACAGCTGCGCGCGCTGCCCCGCCAACATG
TGCAAGCACAAGGTGCTCAGCGACTACCTGCGCGAGCGGGCCCACGACGGCGTGCACTTC
GAGCGCCTCTTCTACGTGGGCGACGGCGCCAACGACTTCTGCCCCATGGGGCTGCTGGCG
GGCGGCGACGTGGCCTTCCCGCGCCGCGGCTACCCCATGCACCGCCTCATTCAGGAGGCC
CAGAAGGCCGAGCCCAGCTCGTTCCGCGCCAGCGTGGTGCCCTGGGAAACGGCTGCAGAT
GTGCGCCTCCACCTGCAACAGGTGCTGAAGTCGTGCTGA
Chromosome Location
17
Locus
17q21.32
External Identifiers
ResourceLink
UniProtKB IDQ8TCT1
UniProtKB Entry NamePHOP1_HUMAN
GenBank Protein ID20196839
GenBank Gene IDAJ457189
GeneCard IDPHOSPHO1
GenAtlas IDPHOSPHO1
HGNC IDHGNC:16815
KEGG IDhsa:162466
NCBI Gene ID162466
General References
  1. Houston B, Paton IR, Burt DW, Farquharson C: Chromosomal localization of the chicken and mammalian orthologues of the orphan phosphatase PHOSPHO1 gene. Anim Genet. 2002 Dec;33(6):451-4. [Article]
  2. Roberts SJ, Owen HC, Farquharson C: Identification of a novel splice variant of the haloacid dehalogenase: PHOSPHO1. Biochem Biophys Res Commun. 2008 Jul 11;371(4):872-6. doi: 10.1016/j.bbrc.2008.04.163. Epub 2008 May 8. [Article]
  3. Zody MC, Garber M, Adams DJ, Sharpe T, Harrow J, Lupski JR, Nicholson C, Searle SM, Wilming L, Young SK, Abouelleil A, Allen NR, Bi W, Bloom T, Borowsky ML, Bugalter BE, Butler J, Chang JL, Chen CK, Cook A, Corum B, Cuomo CA, de Jong PJ, DeCaprio D, Dewar K, FitzGerald M, Gilbert J, Gibson R, Gnerre S, Goldstein S, Grafham DV, Grocock R, Hafez N, Hagopian DS, Hart E, Norman CH, Humphray S, Jaffe DB, Jones M, Kamal M, Khodiyar VK, LaButti K, Laird G, Lehoczky J, Liu X, Lokyitsang T, Loveland J, Lui A, Macdonald P, Major JE, Matthews L, Mauceli E, McCarroll SA, Mihalev AH, Mudge J, Nguyen C, Nicol R, O'Leary SB, Osoegawa K, Schwartz DC, Shaw-Smith C, Stankiewicz P, Steward C, Swarbreck D, Venkataraman V, Whittaker CA, Yang X, Zimmer AR, Bradley A, Hubbard T, Birren BW, Rogers J, Lander ES, Nusbaum C: DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage. Nature. 2006 Apr 20;440(7087):1045-9. [Article]
  4. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
  5. Roberts SJ, Stewart AJ, Sadler PJ, Farquharson C: Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities. Biochem J. 2004 Aug 15;382(Pt 1):59-65. [Article]
  6. Houston B, Stewart AJ, Farquharson C: PHOSPHO1-A novel phosphatase specifically expressed at sites of mineralisation in bone and cartilage. Bone. 2004 Apr;34(4):629-37. [Article]
  7. Stewart AJ, Schmid R, Blindauer CA, Paisey SJ, Farquharson C: Comparative modelling of human PHOSPHO1 reveals a new group of phosphatases within the haloacid dehalogenase superfamily. Protein Eng. 2003 Dec;16(12):889-95. [Article]
  8. Roberts SJ, Stewart AJ, Schmid R, Blindauer CA, Bond SR, Sadler PJ, Farquharson C: Probing the substrate specificities of human PHOSPHO1 and PHOSPHO2. Biochim Biophys Acta. 2005 Aug 31;1752(1):73-82. [Article]

Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
Cholineapproved, nutraceuticalunknowntargetproduct ofDetails