(5R)-carbapenem-3-carboxylate synthase

Details

Name
(5R)-carbapenem-3-carboxylate synthase
Kind
protein
Synonyms
  • 1.14.20.3
  • Carbapenem synthase
Gene Name
carC
UniProtKB Entry
Q9XB59Swiss-Prot
Organism
Pectobacterium carotovorum subsp. carotovorum
NCBI Taxonomy ID
555
Amino acid sequence
>lcl|BSEQ0012001|(5R)-carbapenem-3-carboxylate synthase
MSEIVKFNPVMASGFGAYIDHRDFLEAKTETIKNLLMRQGFVVVKNLDIDSDTFRDIYSA
YGTIVEYADEKIGVGFGYRDTLKLEGEKGKIVTGRGQLPFHADGGLLLSQVDQVFLYAAE
IKNVKFRGATTVCDHALACQEMPAHLLRVLEEETFEVRVLERGYYVDVSPDGWFKVPVFT
DLGWVRKMLIYFPFDEGQPASWEPRIVGFTDHETQAFFQELGAFLKQPRYYYKHFWEDGD
LLIMDNRRVIHEREEFNDDDIVRRLYRGQTADI
Number of residues
273
Molecular Weight
31610.68
Theoretical pI
4.81
GO Classification
Functions
dioxygenase activity / metal ion binding
Processes
antibiotic biosynthetic process
Components
cytoplasm
General Function
Catalyzes the Fe(2+) and alpha-ketoglutarate-dependent conversion of (3S,5S)-carbapenam to (5R)-carbapenem, an essential step in carbapenem antibiotic biosynthesis.
Specific Function
dioxygenase activity
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Cytoplasm
Gene sequence
>lcl|BSEQ0005575|822 bp
ATGAGCGAAATAGTGAAGTTTAATCCGGTCATGGCATCCGGTTTTGGCGCGTATATCGAT
CATCGGGACTTTCTCGAAGCCAAGACAGAAACGATTAAAAACTTACTGATGCGTCAGGGA
TTTGTCGTGGTCAAAAATCTCGATATTGATAGTGACACATTCCGCGATATCTACTCGGCT
TACGGTACGATCGTGGAGTATGCGGATGAAAAGATCGGCGTAGGTTTCGGCTATCGCGAT
ACGTTGAAGCTGGAAGGGGAAAAAGGAAAAATCGTTACCGGACGTGGTCAACTTCCTTTC
CACGCTGATGGCGGCCTGCTGCTGTCACAGGTAGATCAGGTTTTCCTCTACGCGGCCGAG
ATTAAAAACGTCAAATTCCGTGGTGCAACAACGGTATGCGACCATGCTCTGGCTTGTCAG
GAAATGCCGGCTCACCTTCTGCGCGTACTGGAAGAGGAAACGTTCGAAGTTCGTGTGCTA
GAGCGGGGCTATTACGTGGATGTTTCACCAGACGGTTGGTTCAAGGTGCCGGTCTTCACC
GATCTGGGATGGGTCAGAAAGATGCTGATTTATTTCCCATTTGACGAAGGACAACCGGCT
AGCTGGGAGCCGCGGATTGTCGGTTTCACCGATCATGAAACCCAGGCATTCTTCCAGGAA
CTCGGGGCGTTTTTAAAACAGCCACGCTACTACTACAAACACTTCTGGGAAGATGGTGAC
CTGCTGATTATGGACAACCGTCGTGTCATTCATGAGCGTGAAGAGTTTAACGATGACGAC
ATCGTACGTCGTCTGTATCGCGGACAAACCGCCGATATCTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDQ9XB59
UniProtKB Entry NameCARC_PECCC
GenBank Gene IDU17224
PDB ID(s)1NX4, 1NX8, 4OJ8
General References
  1. McGowan SJ, Sebaihia M, O'Leary S, Hardie KR, Williams P, Stewart GS, Bycroft BW, Salmond GP: Analysis of the carbapenem gene cluster of Erwinia carotovora: definition of the antibiotic biosynthetic genes and evidence for a novel beta-lactam resistance mechanism. Mol Microbiol. 1997 Nov;26(3):545-56. [Article]
  2. Stapon A, Li R, Townsend CA: Carbapenem biosynthesis: confirmation of stereochemical assignments and the role of CarC in the ring stereoinversion process from L-proline. J Am Chem Soc. 2003 Jul 16;125(28):8486-93. [Article]
  3. Sleeman MC, Smith P, Kellam B, Chhabra SR, Bycroft BW, Schofield CJ: Biosynthesis of carbapenem antibiotics: new carbapenam substrates for carbapenem synthase (CarC). Chembiochem. 2004 Jun 7;5(6):879-82. [Article]
  4. Clifton IJ, Doan LX, Sleeman MC, Topf M, Suzuki H, Wilmouth RC, Schofield CJ: Crystal structure of carbapenem synthase (CarC). J Biol Chem. 2003 Jun 6;278(23):20843-50. Epub 2003 Feb 28. [Article]

Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
N-AcetylprolineexperimentalunknowntargetDetails