1-phosphatidylinositol phosphodiesterase

Details

Name
1-phosphatidylinositol phosphodiesterase
Kind
protein
Synonyms
  • 4.6.1.13
  • Phosphatidylinositol diacylglycerol-lyase
  • Phosphatidylinositol-specific phospholipase C
  • PI-PLC
  • pic
Gene Name
plcA
UniProtKB Entry
P34024Swiss-Prot
Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
NCBI Taxonomy ID
169963
Amino acid sequence
>lcl|BSEQ0012068|1-phosphatidylinositol phosphodiesterase
MYKNYLQRTLVLLLCFILYFFTFPLGGKAYSLNNWNKPIKNSVTTKQWMSALPDTTNLAA
LSIPGTHDTMSYNGDITWTLTKPLAQTQTMSLYQQLEAGIRYIDIRAKDNLNIYHGPIFL
NASLSGVLETITQFLKKNPKETIIMRLKDEQNSNDSFDYRIQPLINIYKDYFYTTPRTDT
SNKIPTLKDVRGKILLLSENHTKKPLVINSRKFGMQFGAPNQVIQDDYNGPSVKTKFKEI
VQTAYQASKADNKLFLNHISATSLTFTPRQYAAALNNKVEQFVLNLTSEKVRGLGILIMD
FPEKQTIKNIIKNNKFN
Number of residues
317
Molecular Weight
36317.61
Theoretical pI
10.14
GO Classification
Functions
phosphatidylinositol diacylglycerol-lyase activity / phosphoric diester hydrolase activity
Processes
lipid catabolic process / pathogenesis
Components
cytoplasm / extracellular region
General Function
Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Important factor in pathogenesis, PI-PLC activity is present only in virulent listeria species. It may participate in the lysis of the phagolysosomal membrane.
Specific Function
phosphatidylinositol diacylglycerol-lyase activity
Pfam Domain Function
Signal Regions
1-22
Transmembrane Regions
Not Available
Cellular Location
Secreted
Gene sequence
>lcl|BSEQ0012069|1-phosphatidylinositol phosphodiesterase (plcA)
TTGTATAAGAATTATTTGCAACGCACATTAGTTTTATTACTCTGTTTTATTTTATACTTT
TTTACTTTCCCATTAGGCGGAAAAGCATATTCGCTTAATAACTGGAATAAGCCAATAAAG
AACTCTGTAACTACAAAACAATGGATGTCCGCTCTACCTGACACAACAAACCTAGCAGCG
CTCTCTATACCAGGTACACATGATACGATGAGCTATAACGGAGACATAACATGGACATTA
ACCAAACCACTAGCTCAAACACAAACGATGTCATTGTACCAACAACTAGAAGCAGGAATA
CGGTACATCGATATTAGAGCAAAAGACAATCTCAACATTTACCATGGGCCAATTTTTTTA
AATGCATCACTTTCAGGTGTATTAGAAACGATTACTCAATTTTTAAAGAAAAATCCAAAA
GAAACCATTATTATGCGTTTAAAAGACGAGCAAAACAGCAACGATAGTTTTGATTATCGG
ATCCAACCACTAATCAACATTTATAAAGATTATTTTTACACTACTCCCAGAACTGACACG
AGCAATAAAATCCCTACATTAAAAGATGTCCGCGGAAAAATATTATTACTTTCAGAGAAC
CACACAAAAAAGCCATTAGTCATTAACTCACGCAAATTCGGCATGCAGTTCGGCGCACCT
AACCAAGTAATTCAAGATGACTACAATGGTCCGAGTGTGAAAACAAAATTCAAAGAGATT
GTCCAGACTGCTTATCAAGCTTCCAAAGCGGACAATAAACTTTTTCTTAACCATATTAGC
GCCACTTCATTAACATTCACACCTCGTCAGTATGCTGCAGCATTAAACAACAAAGTAGAG
CAATTCGTACTCAACTTAACATCGGAAAAAGTTCGAGGATTAGGCATACTAATCATGGAC
TTCCCCGAAAAACAAACAATTAAAAACATCATAAAAAACAATAAATTCAACTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP34024
UniProtKB Entry NamePLC_LISMO
GenBank Gene IDX54618
PDB ID(s)1AOD, 2PLC
KEGG IDlmo:lmo0201
NCBI Gene ID987032
General References
  1. Mengaud J, Braun-Breton C, Cossart P: Identification of phosphatidylinositol-specific phospholipase C activity in Listeria monocytogenes: a novel type of virulence factor? Mol Microbiol. 1991 Feb;5(2):367-72. [Article]
  2. Leimeister-Wachter M, Domann E, Chakraborty T: Detection of a gene encoding a phosphatidylinositol-specific phospholipase C that is co-ordinately expressed with listeriolysin in Listeria monocytogenes. Mol Microbiol. 1991 Feb;5(2):361-6. [Article]
  3. Glaser P, Frangeul L, Buchrieser C, Rusniok C, Amend A, Baquero F, Berche P, Bloecker H, Brandt P, Chakraborty T, Charbit A, Chetouani F, Couve E, de Daruvar A, Dehoux P, Domann E, Dominguez-Bernal G, Duchaud E, Durant L, Dussurget O, Entian KD, Fsihi H, Garcia-del Portillo F, Garrido P, Gautier L, Goebel W, Gomez-Lopez N, Hain T, Hauf J, Jackson D, Jones LM, Kaerst U, Kreft J, Kuhn M, Kunst F, Kurapkat G, Madueno E, Maitournam A, Vicente JM, Ng E, Nedjari H, Nordsiek G, Novella S, de Pablos B, Perez-Diaz JC, Purcell R, Remmel B, Rose M, Schlueter T, Simoes N, Tierrez A, Vazquez-Boland JA, Voss H, Wehland J, Cossart P: Comparative genomics of Listeria species. Science. 2001 Oct 26;294(5543):849-52. [Article]
  4. Mengaud J, Vicente MF, Cossart P: Transcriptional mapping and nucleotide sequence of the Listeria monocytogenes hlyA region reveal structural features that may be involved in regulation. Infect Immun. 1989 Dec;57(12):3695-701. [Article]
  5. Moser J, Gerstel B, Meyer JE, Chakraborty T, Wehland J, Heinz DW: Crystal structure of the phosphatidylinositol-specific phospholipase C from the human pathogen Listeria monocytogenes. J Mol Biol. 1997 Oct 17;273(1):269-82. [Article]

Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
scyllo-inositolinvestigationalunknowntargetDetails