Blasticidin-S deaminase
Details
- Name
- Blasticidin-S deaminase
- Kind
- protein
- Synonyms
- 3.5.4.23
- Gene Name
- bsd
- UniProtKB Entry
- P0C2P0Swiss-Prot
- Organism
- Aspergillus terreus
- NCBI Taxonomy ID
- 33178
- Amino acid sequence
>lcl|BSEQ0012352|Blasticidin-S deaminase MPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVNVYHFTGGPCAELVVL GTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKAIVKDSDGQPTAVGIRE LLPSGYVWEG
- Number of residues
- 130
- Molecular Weight
- 13468.145
- Theoretical pI
- 4.57
- GO Classification
- Functionsblasticidin-S deaminase activity / zinc ion bindingProcessesresponse to antibiotic
- General Function
- Catalyzes the deamination of the cytosine moiety of the antibiotics blasticidin S, cytomycin and acetylblasticidin S.
- Specific Function
- blasticidin-S deaminase activity
- Pfam Domain Function
- dCMP_cyt_deam_1 (PF00383)
- Signal Regions
- Not Available
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0006563|393 bp ATGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAGAGCAACGGCTACAATCAACAGC ATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCAGCTCTCTCTAGCGACGGCCGCATC TTCACTGGTGTCAATGTATATCATTTTACTGGGGGACCTTGTGCAGAACTCGTGGTGCTG GGCACTGCTGCTGCTGCGGCAGCTGGCAACCTGACTTGTATCGTCGCGATCGGAAATGAG AACAGGGGCATCTTGAGCCCCTGCGGACGGTGTCGACAGGTGCTTCTCGATCTGCATCCT GGGATCAAAGCGATAGTGAAGGACAGTGATGGACAGCCGACGGCAGTTGGGATTCGTGAA TTGCTGCCCTCTGGTTATGTGTGGGAGGGCTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P0C2P0 UniProtKB Entry Name BSD_ASPTE GenBank Gene ID D83710 PDB ID(s) 1WN5, 1WN6, 2Z3G, 2Z3H, 2Z3I, 2Z3J - General References
- Kimura M, Kamakura T, Tao QZ, Kaneko I, Yamaguchi I: Cloning of the blasticidin S deaminase gene (BSD) from Aspergillus terreus and its use as a selectable marker for Schizosaccharomyces pombe and Pyricularia oryzae. Mol Gen Genet. 1994 Jan;242(2):121-9. [Article]
- Kimura M, Sekido S, Isogai Y, Yamaguchi I: Expression, purification, and characterization of blasticidin S deaminase (BSD) from Aspergillus terreus: the role of catalytic zinc in enzyme structure. J Biochem. 2000 Jun;127(6):955-63. [Article]
- Nakasako M, Kimura M, Yamaguchi I: Crystallization and preliminary X-ray diffraction studies of blasticidin S deaminase from Aspergillus terreus. Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):547-8. [Article]
- Kumasaka T, Yamamoto M, Furuichi M, Nakasako M, Teh AH, Kimura M, Yamaguchi I, Ueki T: Crystal structures of blasticidin S deaminase (BSD): implications for dynamic properties of catalytic zinc. J Biol Chem. 2007 Dec 21;282(51):37103-11. Epub 2007 Oct 23. [Article]
Associated Data
- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details TETRAHEDRAL INTERMEDIATE OF BLASTICIDIN S experimental unknown target Details