DNA polymerase I
Details
- Name
- DNA polymerase I
- Kind
- protein
- Synonyms
- 2.7.7.7
- pol
- POL I
- Gene Name
- polA
- UniProtKB Entry
- P52026Swiss-Prot
- Organism
- Geobacillus stearothermophilus
- NCBI Taxonomy ID
- 1422
- Amino acid sequence
>lcl|BSEQ0007417|DNA polymerase I MKNKLVLIDGNSVAYRAFFALPLLHNDKGIHTNAVYGFTMMLNKILAEEQPTHILVAFDA GKTTFRHETFQDYKGGRQQTPPELSEQFPLLRELLKAYRIPAYELDHYEADDIIGTMAAR AEREGFAVKVISGDRDLTQLASPQVTVEITKKGITDIESYTPETVVEKYGLTPEQIVDLK GLMGDKSDNIPGVPGIGEKTAVKLLKQFGTVENVLASIDEIKGEKLKENLRQYRDLALLS KQLAAICRDAPVELTLDDIVYKGEDREKVVALFQELGFQSFLDKMAVQTDEGEKPLAGMD FAIADSVTDEMLADKAALVVEVVGDNYHHAPIVGIALANERGRFFLRPETALADPKFLAW LGDETKKKTMFDSKRAAVALKWKGIELRGVVFDLLLAAYLLDPAQAAGDVAAVAKMHQYE AVRSDEAVYGKGAKRTVPDEPTLAEHLVRKAAAIWALEEPLMDELRRNEQDRLLTELEQP LAGILANMEFTGVKVDTKRLEQMGAELTEQLQAVERRIYELAGQEFNINSPKQLGTVLFD KLQLPVLKKTKTGYSTSADVLEKLAPHHEIVEHILHYRQLGKLQSTYIEGLLKVVHPVTG KVHTMFNQALTQTGRLSSVEPNLQNIPIRLEEGRKIRQAFVPSEPDWLIFAADYSQIELR VLAHIAEDDNLIEAFRRGLDIHTKTAMDIFHVSEEDVTANMRRQAKAVNFGIVYGISDYG LAQNLNITRKEAAEFIERYFASFPGVKQYMDNIVQEAKQKGYVTTLLHRRRYLPDITSRN FNVRSFAERTAMNTPIQGSAADIIKKAMIDLSVRLREERLQARLLLQVHDELILEAPKEE IERLCRLVPEVMEQAVTLRVPLKVDYHYGPTWYDAK
- Number of residues
- 876
- Molecular Weight
- 98669.425
- Theoretical pI
- 5.77
- GO Classification
- Functions3'-5' exonuclease activity / DNA binding / DNA-directed DNA polymerase activityProcessesDNA repair / DNA-dependent DNA replication
- General Function
- In addition to polymerase activity, the recombinant enzyme has strand displacement and 5'-3' exonuclease activity, but lacks proofreading 3'-5' exonuclease activity.
- Specific Function
- 3'-5' exonuclease activity
- Pfam Domain Function
- Signal Regions
- Not Available
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasmic
- Gene sequence
>lcl|BSEQ0007416|2865 bp GTGGCGTCGACAAGGCGCGCAGCCGCAACTCAGGCGGGACGGGCTGGGCCCTTCGATCGT CAAGCACTTGGTCGAGGCGCATCGCGGCTACATTACGGTGACGAGCGAAGTCGGGCGCGG CACCGAGTTTACGATTCATTTCCCGAAGCCGGAGCGGTAGCCGGCTTCTTTTTATGGCCC CCGCCGGCGTGGTACAATAGAACAAGGAACGTCCGAGGAGGGATGATGTTGAAAAACAAG CTCGTCTTAATTGACGGCAACAGCGTGGCGTACCGCGCCTTTTTTGCGTTGCCGCTTTTG CATAACGATAAAGGGATTCATACGAACGCAGTCTACGGGTTTACGATGATGTTAAACAAA ATTTTGGCGGAAGAGCAGCCGACCCACATTCTCGTTGCGTTTGACGCCGGGAAAACGACG TTCCGCCATGAAACGTTCCAAGACTATAAAGGCGGGCGGCAGCAGACGCCGCCGGAACTG TCGGAACAGTTTCCGCTCGTGCGCGAATTGCTCAAAGCGTACCGCATCCCCGCCTATGAG CTCGACCATTATGAAGCGGATGACATCATCGGAACGATGGCGGCGCGGGCTGAGCGAGAA GGGTTTGCAGTGAAAGTCATTTCCGGCGACCGCGATTTAACCCAGCTTGCTTCCCCGCAA GTGACGGTGGAGATTACGAAAAAAGGGATTACCGACATCGAGTCGTACACGCCGGAGACG GTCGTGGAAAAATACGGCCTCACCCCGGAGCAAATTGTCGACTTGAAAGGATTGATGGGC GACAAATCCGACAACATCCCTGGCGTGCCCGGCATCGGGAAAAAAACAGCCGTCAAGCTG CTCAAGCAATTCGGCACGGTCGAAAACGTACTGGCATCGATCGATGAGATCAAAGGGGAG AAGCTGAAAGAAAATTTGCGCCAATACCGGGATTTGGCGCTTTTAAGCAAACAGCTGGCC GCTATTTGCCGCGACGCCCCGGTTGAGCTGACGCTCGATGACATTGTCTACAAAGGAGAA GACCGGGAAAAAGTGGTCGCCTTGTTTCAGGAGCTCGGATTCCAGTCGTTTCTCGACAAG ATGGCCGTCCAAACGGATGAAGGCGAAAAGCCGCTCGCCGGGATGGATTTTGCGATCGCC GACAGCGTCACGGACGAAATGCTCGCCGACAAAGCGGCCCTCGTCGTGGAGGTGGTGGGC GACAACTATCACCATGCCCCGATTGTCGGGATCGCCTTGGCCAACGAACGCGGGCGGTTT TTCCTGCGCCCGGAGACGGCCGTCGCCGATCCGAAATTTCTCGCTTGGCTTGGCGATGAG ACGAAGAAAAAAACGATGTTTGATTCAAAGCGGGCGGCCGTCGCGCTAAATGGGAAAGGA ATCGAACTGGCTGGCGTCGGCGTCGTGTTCGATCTGTTGCTGGCCGCTTACTTGCTCGAT CCGGCGCAGGCGGCGGGCGACGTTGCCGCGGTGGCGAAAATGCATCAGTACGAGGCGGTG CGATCGGATGAGGCGGTCTATGGAAAAGGAGCGAAGCGGACGGTTCCTGATGAACCGACG CTTGCCGAGCAGCTCGTCCGCAAGGCGGCGGCCATTTGGGCGCTTGAAGAGCCGTTGATG GACGAACTGCGCCGCAACGAACAAGATCGGCTGCTGACCGAGCTCGAACACGCGCTGGCT GGCATTTTGGCCAATATGGAATTTACTGGAGTGAAAGTGGACACGAAGCGGCTTGAACAG ATGGGGGCGGAGCTCACCGAGCAGCTGCAGGCGGTCGAGCGGCGCATTTACGAACTCGCC GGCCAAGAGTTCAACATTAACTCGCCGAAACAGCTCGGGACGGTTTTATTTGACAAGCTG CAGCTCCCGGTGTTGAAAAAGACAAAAACCGGCTATTCGACTTCAGCCGATGTGCTAGAA AAGCTTGCACCGCACCATGAAATCGTCGAACATATTTTGCATTACCGCCAACTCGGCAAG CTGCAGTCAACGTATATTGAAGGGCTGCTGAAAGTGGTGCACCCCGTGACGGGCAAAGTG CACACGATGTTCAATCAGGCGTTGACGCAAACCGGGCGCCTCAGCTCCGTCGAACCGAAT TTGCAAAACATTCCGATTCGGCTTGAGGAAGGGCGGAAAATCCGCCAGGCGTTCGTGCCG TCGGAGCCGGACTGGCTCATCTTTGCGGCCGACTATTCGCAAATCGAGCTGCGCGTCCTC GCCCATATCGCGGAAGATGACAATTTGATTGAAGCGTTCCGGCGCTGGTTGGACATCCAT ACGAAAACAGCCATGGACATTTTCCATGTGAGCGAAGAAGACGTGACAGCCAACATGCGC CGCCAAGCGAAGGCCGTCAATTTTGGCATCGTGTACGGCATTAGTGATTACGGTCTGGCG CAAAACTTGAACATTACGCGCAAAGAAGCGGCTGAATTTATTGAGCGATATTTTGCCAGT TTTCCAGGTGTAAAGCAATATATGGACAACATTGTGCAAGAAGCGAAACAAAAAGGGTAT GTGACGACGCTGCTGCATCGGCGCCGCTATTTGCCCGATATTACAAGCCGCAACTTCAAC GTCCGCACGTTCGCCGAGCGGACGGCGATGAACACACCGATCCAGGGATCCGCTGCCGAC ATCATTAAGAAAGCGATGATCGATCTAAGCGTGAGCGTGCGCGAAGAACGGCTGCAGGCG CGCCTGTTGCTGCAAGGTCATGACGAACTCATTTTGGAGGCGCCGAAAGAGGAAATCGGA CGGCTGTGCCGCCTCGTTCCGGAAGTGATGGAGCAAGCCGTGACACTTCGCGTGCCGCTG AAAGTCGATTACCATTACGGTCCGACGTGGTACGACGCCAAATAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P52026 UniProtKB Entry Name DPO1_GEOSE GenBank Protein ID 755588 GenBank Gene ID U23149 PDB ID(s) 1L3S, 1L3T, 1L3U, 1L3V, 1L5U, 1LV5, 1NJX, 1NJY, 1NJZ, 1NK0, 1NK4, 1NK5, 1NK6, 1NK7, 1NK8, 1NK9, 1NKB, 1NKC, 1NKE, 1U45, 1U47, 1U48, 1U49, 1U4B, 1UA1, 1XC9, 1XWL, 2BDP, 3BDP, 3EYZ, 3EZ5, 4BDP - General References
- Phang SM, Teo CY, Lo E, Wong VW: Cloning and complete sequence of the DNA polymerase-encoding gene (BstpolI) and characterisation of the Klenow-like fragment from Bacillus stearothermophilus. Gene. 1995 Sep 22;163(1):65-8. [Article]
- Riggs MG, Tudor S, Sivaram M, McDonough SH: Construction of single amino acid substitution mutants of cloned Bacillus stearothermophilus DNA polymerase I which lack 5'-->3' exonuclease activity. Biochim Biophys Acta. 1996 Jun 7;1307(2):178-86. [Article]
- Kiefer JR, Mao C, Hansen CJ, Basehore SL, Hogrefe HH, Braman JC, Beese LS: Crystal structure of a thermostable Bacillus DNA polymerase I large fragment at 2.1 A resolution. Structure. 1997 Jan 15;5(1):95-108. [Article]
- Kiefer JR, Mao C, Braman JC, Beese LS: Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Nature. 1998 Jan 15;391(6664):304-7. [Article]
Associated Data
- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details 1,2,3-TRIHYDROXY-1,2,3,4-TETRAHYDROBENZO[A]PYRENE experimental unknown target Details