Decaprenyl diphosphate synthase
Details
- Name
- Decaprenyl diphosphate synthase
- Kind
- protein
- Synonyms
- 2.5.1.86
- DecaPP
- Decaprenyl pyrophosphate synthase
- Long-chain isoprenyl diphosphate synthase
- Trans,polycis-decaprenyl diphosphate synthase
- Gene Name
- uppS
- UniProtKB Entry
- P9WFF7Swiss-Prot
- Organism
- Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
- NCBI Taxonomy ID
- 83332
- Amino acid sequence
>lcl|BSEQ0051253|Decaprenyl diphosphate synthase MARDARKRTSSNFPQLPPAPDDYPTFPDTSTWPVVFPELPAAPYGGPCRPPQHTSKAAAP RIPADRLPNHVAIVMDGNGRWATQRGLARTEGHKMGEAVVIDIACGAIELGIKWLSLYAF STENWKRSPEEVRFLMGFNRDVVRRRRDTLKKLGVRIRWVGSRPRLWRSVINELAVAEEM TKSNDVITINYCVNYGGRTEITEATREIAREVAAGRLNPERITESTIARHLQRPDIPDVD LFLRTSGEQRSSNFMLWQAAYAEYIFQDKLWPDYDRRDLWAACEEYASRTRRFGSA
- Number of residues
- 296
- Molecular Weight
- 33790.985
- Theoretical pI
- Not Available
- GO Classification
- Functionsdi-trans,poly-cis-decaprenylcistransferase activity / magnesium ion binding / manganese ion binding / polyprenyltransferase activity / trans-octaprenyltranstransferase activity / Z-farnesyl diphosphate synthase activityProcessesgrowth / polyprenol biosynthetic processComponentscytosol / plasma membrane
- General Function
- Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) in the cis configuration with (2Z,6E)-farnesyl diphosphate (Z-FPP or EZ-FPP) generating the 50 carbon product trans,polycis-decaprenyl diphosphate. When (2E,6E)-farnesyl diphosphate (E-FPP or EE-FPP) is used in vitro, both primary products decaprenyl diphosphate and (2E,6E,10E)-geranylgeranyl diphosphate (EEE-GGPP) are synthesized. M.tuberculosis does not synthesize (2E,6E,10Z)-geranylgeranyl diphosphate (EEZ-GGPP) and heptaprenyl diphosphate. Can also accept many different allylic substrates, including E-geranyl diphosphate (E-GPP), neryl diphosphate (NPP), and all-trans-geranyl-geranyl diphosphate.
- Specific Function
- Di-trans,poly-cis-decaprenylcistransferase activity
- Pfam Domain Function
- Prenyltransf (PF01255)
- Signal Regions
- Not Available
- Transmembrane Regions
- Not Available
- Cellular Location
- Cell membrane
- Gene sequence
>lcl|BSEQ0051254|Decaprenyl diphosphate synthase (uppS) GTGGCTAGGGATGCACGGAAGCGGACGTCCAGCAACTTCCCGCAGCTGCCTCCGGCCCCC GACGACTACCCGACCTTTCCTGATACGTCGACGTGGCCCGTCGTCTTCCCGGAGTTGCCG GCAGCGCCCTACGGAGGCCCCTGCCGGCCACCGCAGCACACTTCGAAAGCGGCCGCGCCA CGAATACCGGCCGACCGGCTGCCCAACCATGTCGCCATTGTCATGGACGGCAATGGCCGC TGGGCCACCCAACGTGGGCTGGCCCGCACCGAAGGTCACAAGATGGGCGAGGCGGTGGTG ATCGACATCGCTTGCGGTGCTATCGAACTTGGGATCAAGTGGCTCAGCCTCTACGCCTTC TCCACGGAGAACTGGAAGCGTTCGCCCGAGGAAGTCCGCTTCCTGATGGGCTTCAACCGC GACGTGGTGCGACGGCGCCGAGACACCCTAAAGAAGCTAGGGGTCCGGATCCGGTGGGTG GGTTCACGACCACGGCTGTGGCGTAGCGTCATCAATGAGTTGGCGGTCGCGGAGGAGATG ACCAAAAGCAACGACGTCATCACCATCAACTATTGCGTCAACTACGGCGGACGCACCGAA ATCACCGAAGCCACCCGCGAAATCGCTCGTGAGGTCGCCGCGGGCAGGCTCAACCCGGAG CGGATCACCGAATCCACGATCGCCCGCCACCTGCAGCGACCCGACATTCCCGACGTTGAC CTCTTCCTGCGGACCTCGGGTGAGCAGCGCTCCAGCAACTTCATGCTGTGGCAGGCGGCC TACGCTGAATACATATTCCAGGACAAGCTCTGGCCCGACTATGACCGCCGCGACTTGTGG GCGGCCTGCGAGGAATACGCGTCGCGCACTCGGCGGTTCGGGAGCGCCTAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P9WFF7 UniProtKB Entry Name DPDS_MYCTU PDB ID(s) 2VG2, 2VG3, 2VG4, 4ONC KEGG ID mtu:Rv2361c NCBI Gene ID 888964 - General References
- Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, Harris D, Gordon SV, Eiglmeier K, Gas S, Barry CE 3rd, Tekaia F, Badcock K, Basham D, Brown D, Chillingworth T, Connor R, Davies R, Devlin K, Feltwell T, Gentles S, Hamlin N, Holroyd S, Hornsby T, Jagels K, Krogh A, McLean J, Moule S, Murphy L, Oliver K, Osborne J, Quail MA, Rajandream MA, Rogers J, Rutter S, Seeger K, Skelton J, Squares R, Squares S, Sulston JE, Taylor K, Whitehead S, Barrell BG: Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature. 1998 Jun 11;393(6685):537-44. [Article]
- Crick DC, Schulbach MC, Zink EE, Macchia M, Barontini S, Besra GS, Brennan PJ: Polyprenyl phosphate biosynthesis in Mycobacterium tuberculosis and Mycobacterium smegmatis. J Bacteriol. 2000 Oct;182(20):5771-8. [Article]
- Schulbach MC, Brennan PJ, Crick DC: Identification of a short (C15) chain Z-isoprenyl diphosphate synthase and a homologous long (C50) chain isoprenyl diphosphate synthase in Mycobacterium tuberculosis. J Biol Chem. 2000 Jul 28;275(30):22876-81. [Article]
- Kaur D, Brennan PJ, Crick DC: Decaprenyl diphosphate synthesis in Mycobacterium tuberculosis. J Bacteriol. 2004 Nov;186(22):7564-70. [Article]
- Kelkar DS, Kumar D, Kumar P, Balakrishnan L, Muthusamy B, Yadav AK, Shrivastava P, Marimuthu A, Anand S, Sundaram H, Kingsbury R, Harsha HC, Nair B, Prasad TS, Chauhan DS, Katoch K, Katoch VM, Kumar P, Chaerkady R, Ramachandran S, Dash D, Pandey A: Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3. [Article]
- Wang W, Dong C, McNeil M, Kaur D, Mahapatra S, Crick DC, Naismith JH: The structural basis of chain length control in Rv1086. J Mol Biol. 2008 Aug 1;381(1):129-40. doi: 10.1016/j.jmb.2008.05.060. Epub 2008 Jul 1. [Article]
Associated Data
- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details Geranyl Diphosphate experimental unknown target Details ISOPENTENYL PYROPHOSPHATE experimental unknown target Details