4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase
Details
- Name
- 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase
- Kind
- protein
- Synonyms
- 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase
- 3.7.1.17
- bphD
- HOPDA hydrolase
- MCP hydrolase
- Meta-cleavage product hydrolase
- Gene Name
- hsaD
- UniProtKB Entry
- P9WNH5Swiss-Prot
- Organism
- Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
- NCBI Taxonomy ID
- 83332
- Amino acid sequence
>lcl|BSEQ0051255|4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase MTATEELTFESTSRFAEVDVDGPLKLHYHEAGVGNDQTVVLLHGGGPGAASWTNFSRNIA VLARHFHVLAVDQPGYGHSDKRAEHGQFNRYAAMALKGLFDQLGLGRVPLVGNSLGGGTA VRFALDYPARAGRLVLMGPGGLSINLFAPDPTEGVKRLSKFSVAPTRENLEAFLRVMVYD KNLITPELVDQRFALASTPESLTATRAMGKSFAGADFEAGMMWREVYRLRQPVLLIWGRE DRVNPLDGALVALKTIPRAQLHVFGQCGHWVQVEKFDEFNKLTIEFLGGGR
- Number of residues
- 291
- Molecular Weight
- 31875.21
- Theoretical pI
- Not Available
- GO Classification
- Functions2,6-dioxo-6-phenylhexa-3-enoate hydrolase activity / 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase activity / 4,9-DSHA hydrolase activityProcessesaromatic compound catabolic process / growth of symbiont in host / lipid catabolic process / steroid biosynthetic processComponentscell wall / plasma membrane
- General Function
- Catalyzes the hydrolysis of a carbon-carbon bond in 4,5: 9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate (4,9-DSHA) to yield 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oate (DOHNAA) and 2-hydroxy-hexa-2,4-dienoate (HHD). Is also able to catalyze the hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) and the synthetic analog 8-(2-chlorophenyl)-2-hydroxy-5-methyl-6-oxoocta-2,4-dienoic acid (HOPODA).
- Specific Function
- 2,6-dioxo-6-phenylhexa-3-enoate hydrolase activity
- Pfam Domain Function
- Abhydrolase_6 (PF12697)
- Signal Regions
- Not Available
- Transmembrane Regions
- Not Available
- Cellular Location
- Not Available
- Gene sequence
>lcl|BSEQ0051256|4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase (hsaD) ATGACAGCTACCGAGGAATTGACGTTCGAATCCACCTCGCGCTTTGCGGAAGTGGACGTC GACGGGCCGCTGAAACTGCACTACCACGAGGCCGGCGTGGGCAACGACCAGACGGTGGTG CTACTGCACGGCGGTGGGCCCGGCGCGGCGAGCTGGACGAACTTCTCGCGTAATATCGCG GTGCTGGCGCGGCACTTTCATGTGCTGGCCGTCGACCAGCCCGGTTACGGCCATTCCGAC AAGCGGGCCGAGCACGGCCAGTTCAATCGCTATGCCGCGATGGCGCTGAAGGGGCTCTTC GATCAGCTGGGGCTGGGGCGGGTACCGCTGGTGGGCAACTCGTTGGGCGGGGGAACCGCG GTCCGGTTTGCGCTGGACTACCCGGCCCGGGCAGGACGGTTAGTGCTGATGGGCCCGGGG GGCCTGAGTATCAACCTGTTTGCGCCCGACCCGACCGAGGGAGTCAAACGGCTGTCGAAG TTCTCCGTTGCGCCCACCCGGGAGAACCTCGAGGCGTTCCTGCGGGTCATGGTCTACGAC AAGAACCTGATCACCCCCGAGTTGGTGGATCAGCGGTTTGCGCTGGCCAGCACCCCGGAG TCGTTGACGGCAACACGGGCGATGGGAAAGTCGTTCGCCGGAGCCGACTTCGAGGCCGGC ATGATGTGGCGCGAGGTGTATCGGCTGCGCCAGCCGGTGTTGCTGATCTGGGGTCGTGAG GACCGGGTCAACCCGCTGGACGGCGCGCTGGTTGCGTTGAAAACGATTCCGCGTGCGCAG CTGCACGTATTCGGGCAGTGTGGGCATTGGGTGCAGGTGGAGAAGTTCGACGAGTTCAAC AAGCTGACGATTGAATTTCTGGGAGGTGGCAGATGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P9WNH5 UniProtKB Entry Name HSAD_MYCTU PDB ID(s) 2VF2, 2WUD, 2WUE, 2WUF, 2WUG, 5JZB, 5JZS KEGG ID mtu:Rv3569c NCBI Gene ID 887378 - General References
- Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, Harris D, Gordon SV, Eiglmeier K, Gas S, Barry CE 3rd, Tekaia F, Badcock K, Basham D, Brown D, Chillingworth T, Connor R, Davies R, Devlin K, Feltwell T, Gentles S, Hamlin N, Holroyd S, Hornsby T, Jagels K, Krogh A, McLean J, Moule S, Murphy L, Oliver K, Osborne J, Quail MA, Rajandream MA, Rogers J, Rutter S, Seeger K, Skelton J, Squares R, Squares S, Sulston JE, Taylor K, Whitehead S, Barrell BG: Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature. 1998 Jun 11;393(6685):537-44. [Article]
- Kendall SL, Withers M, Soffair CN, Moreland NJ, Gurcha S, Sidders B, Frita R, Ten Bokum A, Besra GS, Lott JS, Stoker NG: A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis. Mol Microbiol. 2007 Aug;65(3):684-99. [Article]
- Van der Geize R, Yam K, Heuser T, Wilbrink MH, Hara H, Anderton MC, Sim E, Dijkhuizen L, Davies JE, Mohn WW, Eltis LD: A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages. Proc Natl Acad Sci U S A. 2007 Feb 6;104(6):1947-52. Epub 2007 Jan 30. [Article]
- Kelkar DS, Kumar D, Kumar P, Balakrishnan L, Muthusamy B, Yadav AK, Shrivastava P, Marimuthu A, Anand S, Sundaram H, Kingsbury R, Harsha HC, Nair B, Prasad TS, Chauhan DS, Katoch K, Katoch VM, Kumar P, Chaerkady R, Ramachandran S, Dash D, Pandey A: Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3. [Article]
- Lack N, Lowe ED, Liu J, Eltis LD, Noble ME, Sim E, Westwood IM: Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):2-7. Epub 2007 Dec 20. [Article]
- Lack NA, Yam KC, Lowe ED, Horsman GP, Owen RL, Sim E, Eltis LD: Characterization of a carbon-carbon hydrolase from Mycobacterium tuberculosis involved in cholesterol metabolism. J Biol Chem. 2010 Jan 1;285(1):434-43. doi: 10.1074/jbc.M109.058081. Epub 2009 Oct 29. [Article]
Associated Data
- Drug Relations
Drug Drug group Pharmacological action? Type Actions Details (3E)-2,6-DIOXO-6-PHENYLHEX-3-ENOATE experimental unknown target Details