Splicing factor 3A subunit 2

Details

Name
Splicing factor 3A subunit 2
Kind
protein
Synonyms
  • SAP 62
  • SAP62
  • SF3a66
  • Spliceosome-associated protein 62
Gene Name
SF3A2
UniProtKB Entry
Q15428Swiss-Prot
Organism
Humans
NCBI Taxonomy ID
9606
Amino acid sequence
>lcl|BSEQ0049648|Splicing factor 3A subunit 2
MDFQHRPGGKTGSGGVASSSESNRDRRERLRQLALETIDINKDPYFMKNHLGSYECKLCL
TLHNNEGSYLAHTQGKKHQTNLARRAAKEAKEAPAQPAPEKVKVEVKKFVKIGRPGYKVT
KQRDSEMGQQSLLFQIDYPEIAEGIMPRHRFMSAYEQRIEPPDRRWQYLLMAAEPYETIA
FKVPSREIDKAEGKFWTHWNRETKQFFLQFHFKMEKPPAPPSLPAGPPGVKRPPPPLMNG
LPPRPPLPESLPPPPPGGLPLPPMPPTGPAPSGPPGPPQLPPPAPGVHPPAPVVHPPASG
VHPPAPGVHPPAPGVHPPAPGVHPPTSGVHPPAPGVHPPAPGVHPPAPGVHPPAPGVHPP
APGVHPPPSAGVHPQAPGVHPAAPAVHPQAPGVHPPAPGMHPQAPGVHPQPPGVHPSAPG
VHPQPPGVHPSNPGVHPPTPMPPMLRPPLPSEGPGNIPPPPPTN
Number of residues
464
Molecular Weight
49255.355
Theoretical pI
Not Available
GO Classification
Processes
spliceosomal complex assembly / U2-type prespliceosome assembly
Components
nucleus / U2-type precatalytic spliceosome / U2-type spliceosomal complex
General Function
Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:10882114, PubMed:11533230, PubMed:32494006, PubMed:34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed:10882114, PubMed:11533230, PubMed:32494006, PubMed:34822310). Within the 17S U2 SnRNP complex, SF3A2 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex (PubMed:10882114, PubMed:11533230). Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes, including the Bact complex (PubMed:29360106, PubMed:29361316, PubMed:30315277). Interacts directly with the duplex formed by U2 snRNA and the intron (PubMed:29360106)
Specific Function
Rna binding
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Nucleus
Gene sequence
>lcl|BSEQ0049649|Splicing factor 3A subunit 2 (SF3A2)
ATGGACTTCCAGCATCGCCCCGGGGGCAAGACCGGGAGCGGGGGCGTGGCCTCCTCCTCC
GAGAGCAACCGTGACCGCAGGGAGCGCCTCCGGCAGCTGGCCCTGGAGACCATCGACATC
AACAAGGACCCGTACTTCATGAAGAACCACCTGGGCTCCTATGAATGCAAACTCTGCCTG
ACACTTCACAACAATGAGGGGAGCTACCTGGCACATACGCAGGGGAAGAAGCACCAGACC
AACCTGGCCCGGCGAGCAGCCAAGGAGGCCAAGGAGGCCCCTGCCCAGCCCGCGCCTGAG
AAGGTCAAGGTGGAGGTGAAGAAGTTTGTGAAGATCGGCCGCCCGGGCTACAAAGTGACC
AAGCAGAGAGACTCGGAGATGGGCCAGCAGAGCCTCCTCTTCCAGATTGACTACCCTGAG
ATCGCCGAGGGCATCATGCCACGTCACCGCTTCATGTCTGCGTACGAGCAGAGGATCGAG
CCTCCGGACCGGCGCTGGCAGTACCTGCTCATGGCCGCCGAGCCCTACGAGACCATTGCC
TTCAAGGTGCCGAGCAGAGAGATCGACAAGGCGGAGGGCAAGTTCTGGACACACTGGAAC
CGGGAGACCAAGCAGTTCTTCCTCCAGTTCCACTTTAAGATGGAGAAGCCCCCGGCTCCA
CCCAGCCTCCCTGCTGGCCCCCCTGGGGTGAAGCGGCCTCCACCCCCGCTGATGAACGGT
CTGCCCCCTCGGCCACCGCTGCCTGAGTCTTTGCCACCGCCCCCGCCAGGAGGCCTGCCT
CTGCCACCCATGCCCCCCACAGGGCCTGCGCCCTCAGGGCCCCCGGGACCACCCCAGCTA
CCCCCGCCAGCTCCAGGGGTCCACCCCCCGGCCCCAGTGGTGCATCCCCCTGCATCTGGG
GTCCATCCCCCAGCTCCTGGCGTCCACCCCCCAGCTCCTGGCGTCCATCCCCCAGCCCCT
GGGGTCCACCCACCAACCTCTGGGGTCCACCCCCCAGCTCCTGGAGTCCACCCTCCAGCC
CCCGGGGTTCACCCACCAGCCCCCGGAGTCCACCCACCAGCCCCTGGGGTTCACCCACCA
GCCCCAGGGGTCCATCCTCCCCCATCAGCGGGGGTTCACCCCCAGGCCCCGGGGGTGCAC
CCAGCAGCCCCCGCCGTTCACCCTCAGGCCCCAGGGGTGCACCCACCAGCCCCAGGGATG
CACCCTCAGGCCCCGGGGGTCCACCCCCAACCTCCCGGGGTCCATCCGTCGGCTCCTGGG
GTCCACCCTCAGCCTCCGGGAGTTCACCCCTCAAATCCTGGGGTGCACCCCCCAACTCCC
ATGCCCCCAATGCTGAGGCCCCCACTTCCCTCCGAAGGCCCAGGGAACATACCTCCCCCT
CCCCCAACCAACTGA
Chromosome Location
19
Locus
19p13.3
External Identifiers
ResourceLink
UniProtKB IDQ15428
UniProtKB Entry NameSF3A2_HUMAN
GeneCard IDSF3A2
HGNC IDHGNC:10766
PDB ID(s)5Z56, 5Z57, 5Z58, 6AH0, 6AHD, 6FF4, 6FF7, 6QX9, 6Y53, 6Y5Q, 7ABG, 7ABH, 7ABI, 7EVO, 7ONB, 7Q4O, 7Q4P, 7QTT, 7VPX, 8CH6, 8HK1, 8QO9
KEGG IDhsa:8175
NCBI Gene ID8175
General References
  1. Bennett M, Reed R: Correspondence between a mammalian spliceosome component and an essential yeast splicing factor. Science. 1993 Oct 1;262(5130):105-8. [Article]
  2. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5. Epub 2003 Dec 21. [Article]
  3. Grimwood J, Gordon LA, Olsen A, Terry A, Schmutz J, Lamerdin J, Hellsten U, Goodstein D, Couronne O, Tran-Gyamfi M, Aerts A, Altherr M, Ashworth L, Bajorek E, Black S, Branscomb E, Caenepeel S, Carrano A, Caoile C, Chan YM, Christensen M, Cleland CA, Copeland A, Dalin E, Dehal P, Denys M, Detter JC, Escobar J, Flowers D, Fotopulos D, Garcia C, Georgescu AM, Glavina T, Gomez M, Gonzales E, Groza M, Hammon N, Hawkins T, Haydu L, Ho I, Huang W, Israni S, Jett J, Kadner K, Kimball H, Kobayashi A, Larionov V, Leem SH, Lopez F, Lou Y, Lowry S, Malfatti S, Martinez D, McCready P, Medina C, Morgan J, Nelson K, Nolan M, Ovcharenko I, Pitluck S, Pollard M, Popkie AP, Predki P, Quan G, Ramirez L, Rash S, Retterer J, Rodriguez A, Rogers S, Salamov A, Salazar A, She X, Smith D, Slezak T, Solovyev V, Thayer N, Tice H, Tsai M, Ustaszewska A, Vo N, Wagner M, Wheeler J, Wu K, Xie G, Yang J, Dubchak I, Furey TS, DeJong P, Dickson M, Gordon D, Eichler EE, Pennacchio LA, Richardson P, Stubbs L, Rokhsar DS, Myers RM, Rubin EM, Lucas SM: The DNA sequence and biology of human chromosome 19. Nature. 2004 Apr 1;428(6982):529-35. [Article]
  4. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
  5. Yan D, Perriman R, Igel H, Howe KJ, Neville M, Ares M Jr: CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif. Mol Cell Biol. 1998 Sep;18(9):5000-9. [Article]
  6. Das R, Zhou Z, Reed R: Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. Mol Cell. 2000 May;5(5):779-87. [Article]
  7. Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ: Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. RNA. 2002 Apr;8(4):426-39. [Article]
  8. Burkard TR, Planyavsky M, Kaupe I, Breitwieser FP, Burckstummer T, Bennett KL, Superti-Furga G, Colinge J: Initial characterization of the human central proteome. BMC Syst Biol. 2011 Jan 26;5:17. doi: 10.1186/1752-0509-5-17. [Article]
  9. Zhou H, Di Palma S, Preisinger C, Peng M, Polat AN, Heck AJ, Mohammed S: Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res. 2013 Jan 4;12(1):260-71. doi: 10.1021/pr300630k. Epub 2012 Dec 18. [Article]

Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
Copperapproved, investigationalunknowntargetDetails