Carbonic anhydrase 1
Details
- Name
- Carbonic anhydrase 1
- Synonyms
- 4.2.1.1
- CA-I
- CAB
- Carbonate dehydratase I
- Carbonic anhydrase B
- Carbonic anhydrase I
- Cyanamide hydratase CA1
- Gene Name
- CA1
- UniProtKB Entry
- P00915Swiss-Prot
- Organism
- Humans
- NCBI Taxonomy ID
- 9606
- Amino acid sequence
>lcl|BSEQ0010047|Carbonic anhydrase 1 MASPDWGYDDKNGPEQWSKLYPIANGNNQSPVDIKTSETKHDTSLKPISVSYNPATAKEI INVGHSFHVNFEDNDNRSVLKGGPFSDSYRLFQFHFHWGSTNEHGSEHTVDGVKYSAELH VAHWNSAKYSSLAEAASKADGLAVIGVLMKVGEANPKLQKVLDALQAIKTKGKRAPFTNF DPSTLLPSSLDFWTYPGSLTHPPLYESVTWIICKESISVSSEQLAQFRSLLSNVEGDNAV PMQHNNRPTQPLKGRTVRASF
- Number of residues
- 261
- Molecular Weight
- 28870.0
- Theoretical pI
- 7.14
- GO Classification
- Functionsarylesterase activity / carbonate dehydratase activity / hydro-lyase activity / zinc ion bindingProcessesone-carbon metabolic processComponentscytosol / extracellular exosome
- General Function
- Catalyzes the reversible hydration of carbon dioxide (PubMed:10550681, PubMed:16506782, PubMed:16686544, PubMed:16807956, PubMed:17127057, PubMed:17314045, PubMed:17407288, PubMed:18618712, PubMed:19186056, PubMed:19206230). Can hydrate cyanamide to urea (PubMed:10550681)
- Specific Function
- arylesterase activity
- Pfam Domain Function
- Carb_anhydrase (PF00194)
- Signal Regions
- Not Available
- Transmembrane Regions
- Not Available
- Cellular Location
- Cytoplasm
- Gene sequence
>lcl|BSEQ0010048|Carbonic anhydrase 1 (CA1) ATGGCAAGTCCAGACTGGGGATATGATGACAAAAATGGTCCTGAACAATGGAGCAAGCTG TATCCCATTGCCAATGGAAATAACCAGTCCCCTGTTGATATTAAAACCAGTGAAACCAAA CATGACACCTCTCTGAAACCTATTAGTGTCTCCTACAACCCAGCCACAGCCAAAGAAATT ATCAATGTGGGGCATTCCTTCCATGTAAATTTTGAGGACAACGATAACCGATCAGTGCTG AAAGGTGGTCCTTTCTCTGACAGCTACAGGCTCTTTCAGTTCCATTTTCACTGGGGCAGT ACAAATGAGCATGGTTCAGAACATACAGTGGATGGAGTCAAATATTCTGCCGAGCTTCAC GTAGCTCACTGGAATTCTGCAAAGTACTCCAGCCTTGCTGAAGCTGCCTCAAAGGCTGAT GGTTTGGCAGTTATTGGTGTTTTGATGAAGGTTGGTGAGGCCAACCCAAAGCTGCAGAAA GTACTTGATGCCCTCCAAGCAATTAAAACCAAGGGCAAACGAGCCCCATTCACAAATTTT GACCCCTCTACTCTCCTTCCTTCATCCCTGGATTTCTGGACCTACCCTGGCTCTCTGACT CATCCTCCTCTTTATGAGAGTGTAACTTGGATCATCTGTAAGGAGAGCATCAGTGTCAGC TCAGAGCAGCTGGCACAATTCCGCAGCCTTCTATCAAATGTTGAAGGTGATAACGCTGTC CCCATGCAGCACAACAACCGCCCAACCCAACCTCTGAAGGGCAGAACAGTGAGAGCTTCA TTTTGA
- Chromosome Location
- 8
- Locus
- 8q21.2
- External Identifiers
Resource Link UniProtKB ID P00915 UniProtKB Entry Name CAH1_HUMAN GenBank Protein ID 29600 GenBank Gene ID X05014 GeneCard ID CA1 GenAtlas ID CA1 HGNC ID HGNC:1368 PDB ID(s) 1AZM, 1BZM, 1CRM, 1CZM, 1HCB, 1HUG, 1HUH, 1J9W, 1JV0, 2CAB, 2FOY, 2FW4, 2IT4, 2NMX, 2NN1, 2NN7, 3LXE, 3W6H, 3W6I, 4WR7, 4WUP, 4WUQ, 5E2M, 5GMM, 6EVR, 6EX1, 6F3B, 6FAF, 6FAG, 6G3V, 6HWZ, 6I0J, 6I0L, 6SWM, 6XZE, 6XZO, 6XZS, 6XZX, 6XZY, 6Y00, 7PLF, 7Q0D, 7QOB, 7ZL5, 8CDX, 8CDZ KEGG ID hsa:759 IUPHAR/Guide To Pharmacology ID 2597 NCBI Gene ID 759 - General References
- Barlow JH, Lowe N, Edwards YH, Butterworth PH: Human carbonic anhydrase I cDNA. Nucleic Acids Res. 1987 Mar 11;15(5):2386. [Article]
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- Giraud N, Marriq C, Laurent-Tabusse G: [Primary structure of human B erythrocyte carbonic anhydrase. 3. Sequence of CNBr fragment I and III (residues 149-260)]. Biochimie. 1974;56(8):1031-43. [Article]
- Andersson B, Nyman PO, Strid L: Amino acid sequence of human erythrocyte carbonic anhydrase B. Biochem Biophys Res Commun. 1972 Aug 7;48(3):670-7. [Article]
- Lin KT, Deutsch HF: Human carbonic anhydrases. XI. The complete primary structure of carbonic anhydrase B. J Biol Chem. 1973 Mar 25;248(6):1885-93. [Article]
- Lin KT, Deutsch HF: Human carbonic anhydrases. XII. The complete primary structure of the C isozyme. J Biol Chem. 1974 Apr 25;249(8):2329-37. [Article]
- Briganti F, Mangani S, Scozzafava A, Vernaglione G, Supuran CT: Carbonic anhydrase catalyzes cyanamide hydration to urea: is it mimicking the physiological reaction? J Biol Inorg Chem. 1999 Oct;4(5):528-36. [Article]
- Temperini C, Scozzafava A, Vullo D, Supuran CT: Carbonic anhydrase activators. Activation of isozymes I, II, IV, VA, VII, and XIV with l- and d-histidine and crystallographic analysis of their adducts with isoform II: engineering proton-transfer processes within the active site of an enzyme. Chemistry. 2006 Sep 18;12(27):7057-66. [Article]
- Temperini C, Scozzafava A, Vullo D, Supuran CT: Carbonic anhydrase activators. Activation of isoforms I, II, IV, VA, VII, and XIV with L- and D-phenylalanine and crystallographic analysis of their adducts with isozyme II: stereospecific recognition within the active site of an enzyme and its consequences for the drug design. J Med Chem. 2006 May 18;49(10):3019-27. [Article]
- Temperini C, Innocenti A, Scozzafava A, Mastrolorenzo A, Supuran CT: Carbonic anhydrase activators: L-Adrenaline plugs the active site entrance of isozyme II, activating better isoforms I, IV, VA, VII, and XIV. Bioorg Med Chem Lett. 2007 Feb 1;17(3):628-35. Epub 2006 Nov 15. [Article]
- Crocetti L, Maresca A, Temperini C, Hall RA, Scozzafava A, Muhlschlegel FA, Supuran CT: A thiabendazole sulfonamide shows potent inhibitory activity against mammalian and nematode alpha-carbonic anhydrases. Bioorg Med Chem Lett. 2009 Mar 1;19(5):1371-5. doi: 10.1016/j.bmcl.2009.01.038. Epub 2009 Jan 19. [Article]
- Maresca A, Temperini C, Vu H, Pham NB, Poulsen SA, Scozzafava A, Quinn RJ, Supuran CT: Non-zinc mediated inhibition of carbonic anhydrases: coumarins are a new class of suicide inhibitors. J Am Chem Soc. 2009 Mar 4;131(8):3057-62. doi: 10.1021/ja809683v. [Article]
- Di Fiore A, Monti SM, Hilvo M, Parkkila S, Romano V, Scaloni A, Pedone C, Scozzafava A, Supuran CT, De Simone G: Crystal structure of human carbonic anhydrase XIII and its complex with the inhibitor acetazolamide. Proteins. 2009 Jan;74(1):164-75. doi: 10.1002/prot.22144. [Article]
- Burkard TR, Planyavsky M, Kaupe I, Breitwieser FP, Burckstummer T, Bennett KL, Superti-Furga G, Colinge J: Initial characterization of the human central proteome. BMC Syst Biol. 2011 Jan 26;5:17. doi: 10.1186/1752-0509-5-17. [Article]
- Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. [Article]
- Kannan KK, Fridborg K, Bergsten PC, Liljas A, Lovgren S, Petef M, Strandberg B, Waara I, Adler L, Falkbring SO, Gothe PO, Nyman PO: Structure of human carbonic anhydrase B. I. Crystallization and heavy atom modifications. J Mol Biol. 1972 Feb 14;63(3):601-4. [Article]
- Kannan KK, Notstrand B, Fridborg K, Lovgren S, Ohlsson A, Petef M: Crystal structure of human erythrocyte carbonic anhydrase B. Three-dimensional structure at a nominal 2.2-A resolution. Proc Natl Acad Sci U S A. 1975 Jan;72(1):51-5. [Article]
- Kannan KK, Ramanadham M, Jones TA: Structure, refinement, and function of carbonic anhydrase isozymes: refinement of human carbonic anhydrase I. Ann N Y Acad Sci. 1984;429:49-60. [Article]
- Kumar V, Kannan KK, Sathyamurthi P: Differences in anionic inhibition of human carbonic anhydrase I revealed from the structures of iodide and gold cyanide inhibitor complexes. Acta Crystallogr D Biol Crystallogr. 1994 Sep 1;50(Pt 5):731-8. [Article]
- Kumar V, Kannan KK: Enzyme-substrate interactions. Structure of human carbonic anhydrase I complexed with bicarbonate. J Mol Biol. 1994 Aug 12;241(2):226-32. [Article]
- Chakravarty S, Kannan KK: Drug-protein interactions. Refined structures of three sulfonamide drug complexes of human carbonic anhydrase I enzyme. J Mol Biol. 1994 Oct 21;243(2):298-309. [Article]
- Ferraroni M, Tilli S, Briganti F, Chegwidden WR, Supuran CT, Wiebauer KE, Tashian RE, Scozzafava A: Crystal structure of a zinc-activated variant of human carbonic anhydrase I, CA I Michigan 1: evidence for a second zinc binding site involving arginine coordination. Biochemistry. 2002 May 21;41(20):6237-44. [Article]
- Temperini C, Scozzafava A, Supuran CT: Carbonic anhydrase activators: the first X-ray crystallographic study of an adduct of isoform I. Bioorg Med Chem Lett. 2006 Oct 1;16(19):5152-6. Epub 2006 Jul 25. [Article]
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- Temperini C, Innocenti A, Guerri A, Scozzafava A, Rusconi S, Supuran CT: Phosph(on)ate as a zinc-binding group in metalloenzyme inhibitors: X-ray crystal structure of the antiviral drug foscarnet complexed to human carbonic anhydrase I. Bioorg Med Chem Lett. 2007 Apr 15;17(8):2210-5. Epub 2007 Feb 8. [Article]
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- Omoto K, Ueda S, Goriki K, Takahashi N, Misawa S, Pagaran IG: Population genetic studies of the Philippine Negritos. III. Identification of the carbonic anhydrase-1 variant with CA1 Guam. Am J Hum Genet. 1981 Jan;33(1):105-11. [Article]
- Chegwidden WR, Wagner LE, Venta PJ, Bergenhem NC, Yu YS, Tashian RE: Marked zinc activation of ester hydrolysis by a mutation, 67-His (CAT) to Arg (CGT), in the active site of human carbonic anhydrase I. Hum Mutat. 1994;4(4):294-6. [Article]
Associated Data
- Bio-Entities
Bio-Entity Type Carbonic anhydrase 1 (Humans) protein primaryCarbonic anhydrase (Humans) protein - Drug Relations
Drug Drug group Pharmacological action? Type Actions Details Ethoxzolamide withdrawn yes target inhibitor Details Amlodipine approved unknown target inhibitor Details Methazolamide approved yes target inhibitor Details Acetazolamide approved, vet_approved yes target inhibitor Details Diclofenamide approved, investigational yes target inhibitor Details Brinzolamide approved unknown target inhibitor Details Methyclothiazide withdrawn unknown target inhibitor Details Bendroflumethiazide approved unknown target inhibitor Details Benzthiazide approved unknown target inhibitor Details Cyclothiazide approved unknown target inhibitor Details Hydroflumethiazide approved, investigational, withdrawn unknown target inhibitor Details Chlorothiazide approved, vet_approved yes target inhibitor Details Zonisamide approved, investigational unknown target inhibitor Details Trichlormethiazide approved, vet_approved unknown target inhibitor Details Ethinamate approved, illicit, withdrawn yes target inhibitor Details Quinethazone approved yes target inhibitor Details Dorzolamide approved yes target inhibitor Details N-{2-[4-(AMINOSULFONYL)PHENYL]ETHYL}ACETAMIDE experimental yes target inhibitor Details 3-[4-(AMINOSULFONYL)PHENYL]PROPANOIC ACID experimental yes target inhibitor Details ETHYL 3-[4-(AMINOSULFONYL)PHENYL]PROPANOATE experimental yes target inhibitor Details Methocarbamol approved, vet_approved no target inhibitor Details Ellagic acid investigational yes target inhibitor Details Sodium carbonate approved unknown target inhibitor Details Sodium sulfate approved, vet_approved yes target inhibitor Details Topiramate approved unknown target inhibitor Details Zinc approved, investigational unknown enzyme substrate Details Zinc acetate approved, investigational unknown enzyme ligand Details Chlorthalidone approved yes target inhibitor Details Ferulic acid experimental yes target inhibitor Details 5-[(phenylsulfonyl)amino]-1,3,4-thiadiazole-2-sulfonamide experimental yes target inhibitor Details 5-amino-1,3,4-thiadiazole-2-thiol investigational yes target inhibitor Details Sulfate ion experimental, investigational yes target inhibitor Details Phenylboronic acid experimental yes target inhibitor Details Iodide approved, experimental yes target inhibitor Details Nitrate experimental, investigational yes target inhibitor Details indane-5-sulfonamide experimental yes target inhibitor Details 4-(2-AMINOETHYL)BENZENESULFONAMIDE experimental yes target inhibitor Details Saccharin investigational yes target inhibitor Details N-[4-(AMINOSULFONYL)PHENYL]-2-MERCAPTOBENZAMIDE experimental yes target inhibitor Details Hexamethylene diamine experimental yes target inhibitor Details Cadaverine experimental yes target inhibitor Details p-Coumaric acid experimental yes target inhibitor Details Coumarin experimental yes target inhibitor Details Paraoxon experimental yes target inhibitor Details Phenylethane Boronic Acid experimental yes target inhibitor Details Cianidanol approved, withdrawn yes target inhibitor Details trans-2-hydroxycinnamic acid experimental yes target inhibitor Details 4-Nitrophenyl Phosphate experimental yes target inhibitor Details Curcumin approved, investigational yes target inhibitor Details Polmacoxib investigational yes target inhibitor Details Salicylic acid approved, investigational, vet_approved yes target inhibitor Details Phenol approved, experimental yes target inhibitor Details Benzthiazide approved yes target inhibitor Details Cyclothiazide approved yes target inhibitor Details