Lactose permease
Details
- Name
- Lactose permease
- Synonyms
- Lactose-proton symport
- Gene Name
- lacY
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0011396|Lactose permease MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQ PLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNA GAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQFVFWLGSGCALIL AVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFD QQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMS VRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQL AMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVNEVA
- Number of residues
- 417
- Molecular Weight
- 46502.76
- Theoretical pI
- 8.85
- GO Classification
- Functionslactose / lactose binding / sugarProcessescarbohydrate transport / lactose transport / proton transportComponentsintegral component of membrane / integral component of plasma membrane / membrane / plasma membrane
- General Function
- Sugar:proton symporter activity
- Specific Function
- Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).
- Pfam Domain Function
- LacY_symp (PF01306)
- Transmembrane Regions
- 8-34 42-70 75-100 105-129 141-163 167-186 221-249 254-278 289-308 312-334 347-374 378-398
- Cellular Location
- Cell inner membrane
- Gene sequence
>lcl|BSEQ0011397|Lactose permease (lacY) ATGTACTATTTAAAAAACACAAACTTTTGGATGTTCGGTTTATTCTTTTTCTTTTACTTT TTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATGACATCAACCATATC AGCAAAAGTGATACGGGTATTATTTTTGCCGCTATTTCTCTGTTCTCGCTATTATTCCAA CCGCTGTTTGGTCTGCTTTCTGACAAACTCGGGCTGCGCAAATACCTGCTGTGGATTATT ACCGGCATGTTAGTGATGTTTGCGCCGTTCTTTATTTTTATCTTCGGGCCACTGTTACAA TACAACATTTTAGTAGGATCGATTGTTGGTGGTATTTATCTAGGCTTTTGTTTTAACGCC GGTGCGCCAGCAGTAGAGGCATTTATTGAGAAAGTCAGCCGTCGCAGTAATTTCGAATTT GGTCGCGCGCGGATGTTTGGCTGTGTTGGCTGGGCGCTGTGTGCCTCGATTGTCGGCATC ATGTTCACCATCAATAATCAGTTTGTTTTCTGGCTGGGCTCTGGCTGTGCACTCATCCTC GCCGTTTTACTCTTTTTCGCCAAAACGGATGCGCCCTCTTCTGCCACGGTTGCCAATGCG GTAGGTGCCAACCATTCGGCATTTAGCCTTAAGCTGGCACTGGAACTGTTCAGACAGCCA AAACTGTGGTTTTTGTCACTGTATGTTATTGGCGTTTCCTGCACCTACGATGTTTTTGAC CAACAGTTTGCTAATTTCTTTACTTCGTTCTTTGCTACCGGTGAACAGGGTACGCGGGTA TTTGGCTACGTAACGACAATGGGCGAATTACTTAACGCCTCGATTATGTTCTTTGCGCCA CTGATCATTAATCGCATCGGTGGGAAAAACGCCCTGCTGCTGGCTGGCACTATTATGTCT GTACGTATTATTGGCTCATCGTTCGCCACCTCAGCGCTGGAAGTGGTTATTCTGAAAACG CTGCATATGTTTGAAGTACCGTTCCTGCTGGTGGGCTGCTTTAAATATATTACCAGCCAG TTTGAAGTGCGTTTTTCAGCGACGATTTATCTGGTCTGTTTCTGCTTCTTTAAGCAACTG GCGATGATTTTTATGTCTGTACTGGCGGGCAATATGTATGAAAGCATCGGTTTCCAGGGC GCTTATCTGGTGCTGGGTCTGGTGGCGCTGGGCTTCACCTTAATTTCCGTGTTCACGCTT AGCGGCCCCGGCCCGCTTTCCCTGCTGCGTCGTCAGGTGAATGAAGTCGCTTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P02920 UniProtKB Entry Name LACY_ECOLI GenBank Protein ID 146578 GenBank Gene ID J01636 - General References
- Buchel DE, Gronenborn B, Muller-Hill B: Sequence of the lactose permease gene. Nature. 1980 Feb 7;283(5747):541-5. [Article]
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
- Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
- Heijne G: The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology. EMBO J. 1986 Nov;5(11):3021-7. [Article]
- Calamia J, Manoil C: lac permease of Escherichia coli: topology and sequence elements promoting membrane insertion. Proc Natl Acad Sci U S A. 1990 Jul;87(13):4937-41. [Article]
- Ujwal ML, Jung H, Bibi E, Manoil C, Altenbach C, Hubbell WL, Kaback HR: Membrane topology of helices VII and XI in the lactose permease of Escherichia coli studied by lacY-phoA fusion analysis and site-directed spectroscopy. Biochemistry. 1995 Nov 14;34(45):14909-17. [Article]
- King SC, Hansen CL, Wilson TH: The interaction between aspartic acid 237 and lysine 358 in the lactose carrier of Escherichia coli. Biochim Biophys Acta. 1991 Feb 25;1062(2):177-86. [Article]
- Huang AM, Lee JI, King SC, Wilson TH: Amino acid substitution in the lactose carrier protein with the use of amber suppressors. J Bacteriol. 1992 Aug;174(16):5436-41. [Article]
- Kaback HR: The lac permease of Escherichia coli: a prototypic energy-transducing membrane protein. Biochim Biophys Acta. 1990 Jul 25;1018(2-3):160-2. [Article]
- Whitelegge JP, le Coutre J, Lee JC, Engel CK, Prive GG, Faull KF, Kaback HR: Toward the bilayer proteome, electrospray ionization-mass spectrometry of large, intact transmembrane proteins. Proc Natl Acad Sci U S A. 1999 Sep 14;96(19):10695-8. [Article]
- Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G: Global topology analysis of the Escherichia coli inner membrane proteome. Science. 2005 May 27;308(5726):1321-3. [Article]
- Abramson J, Smirnova I, Kasho V, Verner G, Kaback HR, Iwata S: Structure and mechanism of the lactose permease of Escherichia coli. Science. 2003 Aug 1;301(5633):610-5. [Article]