Polymerase acidic protein
Details
- Name
- Polymerase acidic protein
- Synonyms
- 3.1.-.-
- RNA-directed RNA polymerase subunit P2
- Gene Name
- PA
- Organism
- Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
- Amino acid sequence
>lcl|BSEQ0052098|Polymerase acidic protein MEDFVRQCFNPMIVELAEKTMKEYGEDLKIETNKFAAICTHLEVCFMYSDFHFINEQGES IIVELGDPNALLKHRFEIIEGRDRTMAWTVVNSICNTTGAEKPKFLPDLYDYKENRFIEI GVTRREVHIYYLEKANKIKSEKTHIHIFSFTGEEMATKADYTLDEESRARIKTRLFTIRQ EMASRGLWDSFRQSERGEETIEERFEITGTMRKLADQSLPPNFSSLENFRAYVDGFEPNG YIEGKLSQMSKEVNARIEPFLKTTPRPLRLPNGPPCSQRSKFLLMDALKLSIEDPSHEGE GIPLYDAIKCMRTFFGWKEPNVVKPHEKGINPNYLLSWKQVLAELQDIENEEKIPKTKNM KKTSQLKWALGENMAPEKVDFDDCKDVGDLKQYDSDEPELRSLASWIQNEFNKACELTDS SWIELDEIGEDVAPIEHIASMRRNYFTSEVSHCRATEYIMKGVYINTALLNASCAAMDDF QLIPMISKCRTKEGRRKTNLYGFIIKGRSHLRNDTDVVNFVSMEFSLTDPRLEPHKWEKY CVLEIGDMLIRSAIGQVSRPMFLYVRTNGTSKIKMKWGMEMRRCLLQSLQQIESMIEAES SVKEKDMTKEFFENKSETWPIGESPKGVEESSIGKVCRTLLAKSVFNSLYASPQLEGFSA ESRKLLLIVQALRDNLEPGTFDLGGLYEAIEECLINDPWVLLNASWFNSFLTHALS
- Number of residues
- 716
- Molecular Weight
- 82587.575
- Theoretical pI
- Not Available
- GO Classification
- Functionsendonuclease activity / metal ion binding / RNA bindingProcessescap snatching / fusion of virus membrane with host plasma membrane / intracellular transport of virus / receptor-mediated endocytosis of virus by host cell / suppression by virus of host RNA polymerase II activity / transcription, DNA-templated / uncoating of virus / viral budding from plasma membrane / viral genome maturation / viral genome packaging / viral RNA genome replication / viral transcription / virion attachment to host cellComponentscytosol / endosome lumen / extracellular region / host cell cytoplasm / host cell nucleus / nucleoplasm
- General Function
- Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.
- Specific Function
- Endonuclease activity
- Pfam Domain Function
- Flu_PA (PF00603)
- Transmembrane Regions
- Not Available
- Cellular Location
- Host cytoplasm
- Gene sequence
>lcl|BSEQ0052099|Polymerase acidic protein (PA) ATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACA ATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCACT CACTTGGAAGTATGCTTCATGTATTCAGATTTCCACTTCATCAATGAGCAAGGCGAGTCA ATAATCGTAGAACTTGGTGATCCTAATGCACTTTTGAAGCACAGATTTGAAATAATCGAG GGAAGAGATCGCACAATGGCCTGGACAGTAGTAAACAGTATTTGCAACACTACAGGGGCT GAGAAACCAAAGTTTCTACCAGATTTGTATGATTACAAGGAAAATAGATTCATCGAAATT GGAGTAACAAGGAGAGAAGTTCACATATACTATCTGGAAAAGGCCAATAAAATTAAATCT GAGAAAACACACATCCACATTTTCTCGTTCACTGGGGAAGAAATGGCCACAAAGGCCGAC TACACTCTCGATGAAGAAAGCAGGGCTAGGATCAAAACCAGGCTATTCACCATAAGACAA GAAATGGCCAGCAGAGGCCTCTGGGATTCCTTTCGTCAGTCCGAGAGAGGAGAAGAGACA ATTGAAGAAAGGTTTGAAATCACAGGAACAATGCGCAAGCTTGCCGACCAAAGTCTCCCG CCGAACTTCTCCAGCCTTGAAAATTTTAGAGCCTATGTGGATGGATTCGAACCGAACGGC TACATTGAGGGCAAGCTGTCTCAAATGTCCAAAGAAGTAAATGCTAGAATTGAACCTTTT TTGAAAACAACACCACGACCACTTAGACTTCCGAATGGGCCTCCCTGTTCTCAGCGGTCC AAATTCCTGCTGATGGATGCCTTAAAATTAAGCATTGAGGACCCAAGTCATGAAGGAGAG GGAATACCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGAACCC AATGTTGTTAAACCACACGAAAAGGGAATAAATCCAAATTATCTTCTGTCATGGAAGCAA GTACTGGCAGAACTGCAGGACATTGAGAATGAGGAGAAAATTCCAAAGACTAAAAATATG AAAAAAACAAGTCAGCTAAAGTGGGCACTTGGTGAGAACATGGCACCAGAAAAGGTAGAC TTTGACGACTGTAAAGATGTAGGTGATTTGAAGCAATATGATAGTGATGAACCAGAATTG AGGTCGCTTGCAAGTTGGATTCAGAATGAGTTCAACAAGGCATGCGAACTGACAGATTCA AGCTGGATAGAGCTTGATGAGATTGGAGAAGATGTGGCTCCAATTGAACACATTGCAAGC ATGAGAAGGAATTATTTCACATCAGAGGTGTCTCACTGCAGAGCCACAGAATACATAATG AAGGGGGTGTACATCAATACTGCCTTACTTAATGCATCTTGTGCAGCAATGGATGATTTC CAATTAATTCCAATGATAAGCAAGTGTAGAACTAAGGAGGGAAGGCGAAAGACCAACTTG TATGGTTTCATCATAAAAGGAAGATCCCACTTAAGGAATGACACCGACGTGGTAAACTTT GTGAGCATGGAGTTTTCTCTCACTGACCCAAGACTTGAACCACACAAATGGGAGAAGTAC TGTGTTCTTGAGATAGGAGATATGCTTCTAAGAAGTGCCATAGGCCAGGTTTCAAGGCCC ATGTTCTTGTATGTGAGGACAAATGGAACCTCAAAAATTAAAATGAAATGGGGAATGGAG ATGAGGCGTTGTCTCCTCCAGTCACTTCAACAAATTGAGAGTATGATTGAAGCTGAGTCC TCTGTCAAAGAGAAAGACATGACCAAAGAGTTCTTTGAGAACAAATCAGAAACATGGCCC ATTGGAGAGTCTCCCAAAGGAGTGGAGGAAAGTTCCATTGGGAAGGTCTGCAGGACTTTA TTAGCAAAGTCGGTATTTAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCT GAATCAAGAAAACTGCTTCTTATCGTTCAGGCTCTTAGGGACAATCTGGAACCTGGGACC TTTGATCTTGGGGGGCTATATGAAGCAATTGAGGAGTGCCTAATTAATGATCCCTGGGTT TTGCTTAATGCTTCTTGGTTCAACTCCTTCCTTACACATGCATTGAGTTAG
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P03433 UniProtKB Entry Name PA_I34A1 - General References
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- Sanz-Ezquerro JJ, Fernandez Santaren J, Sierra T, Aragon T, Ortega J, Ortin J, Smith GL, Nieto A: The PA influenza virus polymerase subunit is a phosphorylated protein. J Gen Virol. 1998 Mar;79 ( Pt 3):471-8. doi: 10.1099/0022-1317-79-3-471. [Article]
- Fodor E, Smith M: The PA subunit is required for efficient nuclear accumulation of the PB1 subunit of the influenza A virus RNA polymerase complex. J Virol. 2004 Sep;78(17):9144-53. doi: 10.1128/JVI.78.17.9144-9153.2004. [Article]
- Dias A, Bouvier D, Crepin T, McCarthy AA, Hart DJ, Baudin F, Cusack S, Ruigrok RW: The cap-snatching endonuclease of influenza virus polymerase resides in the PA subunit. Nature. 2009 Apr 16;458(7240):914-8. doi: 10.1038/nature07745. Epub 2009 Feb 4. [Article]
- Suzuki T, Ainai A, Nagata N, Sata T, Sawa H, Hasegawa H: A novel function of the N-terminal domain of PA in assembly of influenza A virus RNA polymerase. Biochem Biophys Res Commun. 2011 Nov 4;414(4):719-26. doi: 10.1016/j.bbrc.2011.09.142. Epub 2011 Oct 6. [Article]
- Obayashi E, Yoshida H, Kawai F, Shibayama N, Kawaguchi A, Nagata K, Tame JR, Park SY: The structural basis for an essential subunit interaction in influenza virus RNA polymerase. Nature. 2008 Aug 28;454(7208):1127-31. doi: 10.1038/nature07225. Epub 2008 Jul 27. [Article]
- Fudo S, Yamamoto N, Nukaga M, Odagiri T, Tashiro M, Neya S, Hoshino T: Structural and computational study on inhibitory compounds for endonuclease activity of influenza virus polymerase. Bioorg Med Chem. 2015 Sep 1;23(17):5466-75. doi: 10.1016/j.bmc.2015.07.046. Epub 2015 Jul 29. [Article]
- Fudo S, Yamamoto N, Nukaga M, Odagiri T, Tashiro M, Hoshino T: Two Distinctive Binding Modes of Endonuclease Inhibitors to the N-Terminal Region of Influenza Virus Polymerase Acidic Subunit. Biochemistry. 2016 May 10;55(18):2646-60. doi: 10.1021/acs.biochem.5b01087. Epub 2016 Apr 26. [Article]