2-hydroxymuconate tautomerase

Details

Name
2-hydroxymuconate tautomerase
Synonyms
  • 4-OT
  • 4-oxalocrotonate tautomerase
  • 5.3.2.6
Gene Name
xylH
Organism
Pseudomonas putida
Amino acid sequence
>lcl|BSEQ0016418|2-hydroxymuconate tautomerase
MPIAQIHILEGRSDEQKETLIREVSEAISRSLDAPLTSVRVIITEMAKGHFGIGGELASK
VRR
Number of residues
63
Molecular Weight
6941.95
Theoretical pI
7.72
GO Classification
Functions
isomerase activity
Processes
toluene catabolic process / xylene catabolic process
General Function
Isomerase activity
Specific Function
Catalyzes the ketonization of 2-hydroxymuconate stereoselectively to yield 2-oxo-3-hexenedioate.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0016419|2-hydroxymuconate tautomerase (xylH)
ATGCCTATTGCCCAGATCCACATCCTTGAAGGCCGCAGCGACGAGCAGAAGGAAACCCTC
ATTCGGGAAGTCAGCGAGGCCATCTCGCGCTCCCTGGATGCGCCGCTGACCAGCGTGCGA
GTGATTATCACGGAGATGGCCAAGGGCCACTTCGGCATCGGCGGCGAACTGGCCAGCAAG
GTCAGACGCTGA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDQ01468
UniProtKB Entry Name4OT1_PSEPU
GenBank Protein ID151717
GenBank Gene IDM95650
General References
  1. Chen LH, Kenyon GL, Curtin F, Harayama S, Bembenek ME, Hajipour G, Whitman CP: 4-Oxalocrotonate tautomerase, an enzyme composed of 62 amino acid residues per monomer. J Biol Chem. 1992 Sep 5;267(25):17716-21. [Article]
  2. Harayama S, Rekik M: Comparison of the nucleotide sequences of the meta-cleavage pathway genes of TOL plasmid pWW0 from Pseudomonas putida with other meta-cleavage genes suggests that both single and multiple nucleotide substitutions contribute to enzyme evolution. Mol Gen Genet. 1993 May;239(1-2):81-9. [Article]
  3. Greated A, Lambertsen L, Williams PA, Thomas CM: Complete sequence of the IncP-9 TOL plasmid pWW0 from Pseudomonas putida. Environ Microbiol. 2002 Dec;4(12):856-71. [Article]
  4. Subramanya HS, Roper DI, Dauter Z, Dodson EJ, Davies GJ, Wilson KS, Wigley DB: Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases. Biochemistry. 1996 Jan 23;35(3):792-802. [Article]
  5. Taylor AB, Czerwinski RM, Johnson WH Jr, Whitman CP, Hackert ML: Crystal structure of 4-oxalocrotonate tautomerase inactivated by 2-oxo-3-pentynoate at 2.4 A resolution: analysis and implications for the mechanism of inactivation and catalysis. Biochemistry. 1998 Oct 20;37(42):14692-700. [Article]
  6. Stivers JT, Abeygunawardana C, Mildvan AS, Hajipour G, Whitman CP, Chen LH: Catalytic role of the amino-terminal proline in 4-oxalocrotonate tautomerase: affinity labeling and heteronuclear NMR studies. Biochemistry. 1996 Jan 23;35(3):803-13. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB020052-Oxo-3-Pentenoic AcidexperimentalunknownDetails