Farnesyl diphosphate synthase

Details

Name

Farnesyl diphosphate synthase

Kind

protein

Synonyms

• (2E,6E)-farnesyl diphosphate synthase
• 2.5.1.10
• FPP synthase
• Geranyltranstransferase

Gene Name

ispA

UniProtKB Entry

P22939Swiss-Prot

Organism

Escherichia coli (strain K12)

NCBI Taxonomy ID

83333

Amino acid sequence

>lcl|BSEQ0011072|Farnesyl diphosphate synthase
MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGGKRLRPFLVYATGHMFG
VSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCHVKFGEANAILAGDALQTL
AFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQALDLDAEGKHVPLDALERIHRH
KTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLAFQVQDDILDVVGDTATLGKRQG
ADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQLAEQSLDTSALEALADYIIQRNK

Number of residues

299

Molecular Weight

32159.22

Theoretical pI

5.18

GO Classification

Functions

dimethylallyltranstransferase activity / geranyltranstransferase activity / metal ion binding

Processes

farnesyl diphosphate biosynthetic process / geranyl diphosphate biosynthetic process

Components

cytosol

General Function

Metal ion binding

Specific Function

Not Available

Pfam Domain Function

• polyprenyl_synt (PF00348)

Signal Regions

Not Available

Transmembrane Regions

Not Available

Cellular Location

Cytoplasm

Gene sequence

>lcl|BSEQ0011073|Farnesyl diphosphate synthase (ispA)
ATGGACTTTCCGCAGCAACTCGAAGCCTGCGTTAAGCAGGCCAACCAGGCGCTGAGCCGT
TTTATCGCCCCACTGCCCTTTCAGAACACTCCCGTGGTCGAAACCATGCAGTATGGCGCA
TTATTAGGTGGTAAGCGCCTGCGACCTTTCCTGGTTTATGCCACCGGTCATATGTTCGGC
GTTAGCACAAACACGCTGGACGCACCCGCTGCCGCCGTTGAGTGTATCCACGCTTACTCA
TTAATTCATGATGATTTACCGGCAATGGATGATGACGATCTGCGTCGCGGTTTGCCAACC
TGCCATGTGAAGTTTGGCGAAGCAAACGCGATTCTCGCTGGCGACGCTTTACAAACGCTG
GCGTTCTCGATTTTAAGCGATGCCGATATGCCGGAAGTGTCGGACCGCGACAGAATTTCG
ATGATTTCTGAACTGGCGAGCGCCAGTGGTATTGCCGGAATGTGCGGTGGTCAGGCATTA
GATTTAGACGCGGAAGGCAAACACGTACCTCTGGACGCGCTTGAGCGTATTCATCGTCAT
AAAACCGGCGCATTGATTCGCGCCGCCGTTCGCCTTGGTGCATTAAGCGCCGGAGATAAA
GGACGTCGTGCTCTGCCGGTACTCGACAAGTATGCAGAGAGCATCGGCCTTGCCTTCCAG
GTTCAGGATGACATCCTGGATGTGGTGGGAGATACTGCAACGTTGGGAAAACGCCAGGGT
GCCGACCAGCAACTTGGTAAAAGTACCTACCCTGCACTTCTGGGTCTTGAGCAAGCCCGG
AAGAAAGCCCGGGATCTGATCGACGATGCCCGTCAGTCGCTGAAACAACTGGCTGAACAG
TCACTCGATACCTCGGCACTGGAAGCGCTAGCGGACTACATCATCCAGCGTAATAAATAA

Chromosome Location

Not Available

Locus

Not Available

External Identifiers

Resource	Link
UniProtKB ID	P22939
UniProtKB Entry Name	ISPA_ECOLI
GenBank Protein ID	216583
GenBank Gene ID	D00694
PDB ID(s)	1RQI, 1RQJ
KEGG ID	ecj:JW0411
NCBI Gene ID	945064

General References

1. Fujisaki S, Hara H, Nishimura Y, Horiuchi K, Nishino T: Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in Escherichia coli. J Biochem. 1990 Dec;108(6):995-1000. [Article]
2. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
3. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
4. Hosfield DJ, Zhang Y, Dougan DR, Broun A, Tari LW, Swanson RV, Finn J: Structural basis for bisphosphonate-mediated inhibition of isoprenoid biosynthesis. J Biol Chem. 2004 Mar 5;279(10):8526-9. Epub 2003 Dec 12. [Article]

Associated Data

Drug Relations

Drug	Drug group	Pharmacological action?	Type	Actions	Details
Dimethylallyl S-Thiolodiphosphate	experimental	unknown	target		Details
Isopentyl Pyrophosphate	experimental	unknown	target		Details
Pyrophosphoric acid	approved, experimental	unknown	target	modulator	Details