Ribonuclease Z

Details

Name
Ribonuclease Z
Kind
protein
Synonyms
  • 3.1.26.11
  • RNase Z
  • tRNA 3 endonuclease
  • tRNase Z
  • yqjK
Gene Name
rnz
UniProtKB Entry
P54548Swiss-Prot
Organism
Bacillus subtilis (strain 168)
NCBI Taxonomy ID
224308
Amino acid sequence
>lcl|BSEQ0012224|Ribonuclease Z
MELLFLGTGAGIPAKARNVTSVALKLLEERRSVWLFDCGEATQHQILHTTIKPRKIEKIF
ITHMHGDHVYGLPGLLGSRSFQGGEDELTVYGPKGIKAFIETSLAVTKTHLTYPLAIQEI
EEGIVFEDDQFIVTAVSVIHGVEAFGYRVQEKDVPGSLKADVLKEMNIPPGPVYQKIKKG
ETVTLEDGRIINGNDFLEPPKKGRSVVFSGDTRVSDKLKELARDCDVLVHEATFAKEDRK
LAYDYYHSTTEQAAVTAKEARAKQLILTHISARYQGDASLELQKEAVDVFPNSVAAYDFL
EVNVPRG
Number of residues
307
Molecular Weight
34022.58
Theoretical pI
6.19
GO Classification
Functions
3'-tRNA processing endoribonuclease activity / zinc ion binding
General Function
Zinc ion binding
Specific Function
Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0012225|Ribonuclease Z (rnz)
GTGGAATTACTTTTTTTAGGGACGGGAGCCGGCATCCCCGCCAAGGCGAGAAATGTAACG
TCGGTGGCATTAAAATTGCTTGAAGAAAGGCGTTCGGTATGGCTTTTTGACTGCGGGGAA
GCCACACAGCATCAAATTTTACATACAACGATTAAACCTCGTAAAATAGAGAAAATCTTT
ATTACACACATGCACGGCGATCATGTATACGGACTTCCGGGGCTTCTGGGGAGCCGTTCC
TTTCAAGGCGGAGAGGACGAGCTGACAGTGTACGGACCTAAAGGGATCAAGGCGTTTATT
GAAACAAGCCTTGCCGTCACGAAAACCCATTTGACATATCCGCTTGCGATCCAGGAAATT
GAAGAAGGAATCGTGTTTGAGGACGATCAGTTTATTGTCACAGCGGTATCTGTTATTCAT
GGAGTGGAAGCCTTCGGGTACCGTGTGCAGGAAAAAGACGTACCGGGTTCCTTGAAGGCT
GACGTATTAAAAGAAATGAACATCCCGCCCGGACCTGTATATCAGAAAATCAAAAAAGGC
GAAACGGTAACGCTTGAAGACGGACGAATCATCAATGGGAATGATTTTCTGGAGCCTCCT
AAAAAGGGAAGATCTGTTGTGTTCTCCGGTGATACGAGAGTAAGTGACAAACTAAAAGAG
CTTGCGAGGGATTGTGATGTGCTTGTTCATGAAGCAACCTTTGCTAAGGAAGACCGTAAA
CTTGCTTATGATTATTATCACAGTACAACGGAACAAGCGGCTGTAACAGCGAAAGAAGCA
AGAGCGAAGCAGCTCATTTTAACCCATATCAGCGCAAGATATCAGGGAGATGCTTCTTTG
GAGCTTCAAAAAGAAGCGGTTGACGTTTTCCCCAATAGCGTGGCGGCATATGATTTCTTA
GAGGTAAACGTCCCGCGAGGCTGA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP54548
UniProtKB Entry NameRNZ_BACSU
GenBank Gene IDD84432
PDB ID(s)1Y44, 2FK6, 4GCW
KEGG IDbsu:BSU23840
NCBI Gene ID938694
General References
  1. Mizuno M, Masuda S, Takemaru K, Hosono S, Sato T, Takeuchi M, Kobayashi Y: Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes. Microbiology. 1996 Nov;142 ( Pt 11):3103-11. [Article]
  2. Kunst F, Ogasawara N, Moszer I, Albertini AM, Alloni G, Azevedo V, Bertero MG, Bessieres P, Bolotin A, Borchert S, Borriss R, Boursier L, Brans A, Braun M, Brignell SC, Bron S, Brouillet S, Bruschi CV, Caldwell B, Capuano V, Carter NM, Choi SK, Cordani JJ, Connerton IF, Cummings NJ, Daniel RA, Denziot F, Devine KM, Dusterhoft A, Ehrlich SD, Emmerson PT, Entian KD, Errington J, Fabret C, Ferrari E, Foulger D, Fritz C, Fujita M, Fujita Y, Fuma S, Galizzi A, Galleron N, Ghim SY, Glaser P, Goffeau A, Golightly EJ, Grandi G, Guiseppi G, Guy BJ, Haga K, Haiech J, Harwood CR, Henaut A, Hilbert H, Holsappel S, Hosono S, Hullo MF, Itaya M, Jones L, Joris B, Karamata D, Kasahara Y, Klaerr-Blanchard M, Klein C, Kobayashi Y, Koetter P, Koningstein G, Krogh S, Kumano M, Kurita K, Lapidus A, Lardinois S, Lauber J, Lazarevic V, Lee SM, Levine A, Liu H, Masuda S, Mauel C, Medigue C, Medina N, Mellado RP, Mizuno M, Moestl D, Nakai S, Noback M, Noone D, O'Reilly M, Ogawa K, Ogiwara A, Oudega B, Park SH, Parro V, Pohl TM, Portelle D, Porwollik S, Prescott AM, Presecan E, Pujic P, Purnelle B, Rapoport G, Rey M, Reynolds S, Rieger M, Rivolta C, Rocha E, Roche B, Rose M, Sadaie Y, Sato T, Scanlan E, Schleich S, Schroeter R, Scoffone F, Sekiguchi J, Sekowska A, Seror SJ, Serror P, Shin BS, Soldo B, Sorokin A, Tacconi E, Takagi T, Takahashi H, Takemaru K, Takeuchi M, Tamakoshi A, Tanaka T, Terpstra P, Togoni A, Tosato V, Uchiyama S, Vandebol M, Vannier F, Vassarotti A, Viari A, Wambutt R, Wedler H, Weitzenegger T, Winters P, Wipat A, Yamamoto H, Yamane K, Yasumoto K, Yata K, Yoshida K, Yoshikawa HF, Zumstein E, Yoshikawa H, Danchin A: The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature. 1997 Nov 20;390(6657):249-56. [Article]
  3. Pellegrini O, Nezzar J, Marchfelder A, Putzer H, Condon C: Endonucleolytic processing of CCA-less tRNA precursors by RNase Z in Bacillus subtilis. EMBO J. 2003 Sep 1;22(17):4534-43. [Article]
  4. Li de la Sierra-Gallay I, Pellegrini O, Condon C: Structural basis for substrate binding, cleavage and allostery in the tRNA maturase RNase Z. Nature. 2005 Feb 10;433(7026):657-61. Epub 2005 Jan 16. [Article]
  5. Li de la Sierra-Gallay I, Mathy N, Pellegrini O, Condon C: Structure of the ubiquitous 3' processing enzyme RNase Z bound to transfer RNA. Nat Struct Mol Biol. 2006 Apr;13(4):376-7. Epub 2006 Mar 5. [Article]

Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
2-(N-morpholino)ethanesulfonic acidexperimentalunknowntargetDetails