BoNT/A

Details

Name

BoNT/A

Kind

protein

Synonyms

• boNT/A
• Bont/A1
• BoNT/A1 neurotoxin
• bonta
• Botulinum neurotoxin type A
• Neurotoxin A
• Neurotoxin BoNT
• Neurotoxin type A
• Peptidase M27

Gene Name

bont/a

UniProtKB Entry

Q7B8V4TrEMBL

Organism

Clostridium botulinum

NCBI Taxonomy ID

1491

Amino acid sequence

>lcl|BSEQ0022446|BoNT/A
MPFVNKQFNYKDPVNGVDIAYIKIPNAGQMQPVKAFKIHNKIWVIPERDTFTNPEEGDLN
PPPEAKQVPVSYYDSTYLSTDNEKDNYLKGVTKLFERIYSTDLGRMLLTSIVRGIPFWGG
STIDTELKVIDTNCINVIQPDGSYRSEELNLVIIGPSADIIQFECKSFGHEVLNLTRNGY
GSTQYIRFSPDFTFGFEESLEVDTNPLLGAGKFATDPAVTLAHELIHAGHRLYGIAINPN
RVFKVNTNAYYEMSGLEVSFEELRTFGGHDAKFIDSLQENEFRLYYYNKFKDIASTLNKA
KSIVGTTASLQYMKNVFKEKYLLSEDTSGKFSVDKLKFDKLYKMLTEIYTEDNFVKFFKV
LNRKTYLNFDKAVFKINIVPKVNYTIYDGFNLRNTNLAANFNGQNTEINNMNFTKLKNFT
GLFEFYKLLCVRGIITSKTKSLDKGYNKALNDLCIKVNNWDLFFSPSEDNFTNDLNKGEE
ITSDTNIEAAEENISLDLIQQYYLTFNFDNEPENISIENLSSDIIGQLELMPNIERFPNG
KKYELDKYTMFHYLRAQEFEHGKSRIALTNSVNEALLNPSRVYTFFSSDYVKKVNKATEA
AMFLGWVEQLVYDFTDETSEVSTTDKIADITIIIPYIGPALNIGNMLYKDDFVGALIFSG
AVILLEFIPEIAIPVLGTFALVSYIANKVLTVQTIDNALSKRNEKWDEVYKYIVTNWLAK
VNTQIDLIRKKMKEALENQAEATKAIINYQYNQYTEEEKNNINFNIDDLSSKLNESINKA
MININKFLNQCSVSYLMNSMIPYGVKRLEDFDASLKDALLKYIYDNRGTLIGQVDRLKDK
VNNTLSTDIPFQLSKYVDNQRLLSTFTEYIKNIINTSILNLRYESNHLIDLSRYASKINI
GSKVNFDPIDKNQIQLFNLESSKIEVILKNAIVYNSMYENFSTSFWIRIPKYFNSISLNN
EYTIINCMENNSGWKVSLNYGEIIWTLQDTQEIKQRVVFKYSQMINISDYINRWIFVTIT
NNRLNNSKIYINGRLIDQKPISNLGNIHASNNIMFKLDGCRDTHRYIWIKYFNLFDKELN
EKEIKDLYDNQSNSGILKDFWGDYLQYDKPYYMLNLYDPNKYVDVNNVGIRGYMYLKGPR
GSVMTTNIYLNSSLYRGTKFIIKKYASGNKDNIVRNNDRVYINVVVKNKEYRLATNASQA
GVEKILSALEIPDVGNLSQVVVMKSKNDQGITNKCKMNLQDNNGNDIGFIGFHQFNNIAK
LVASNWYNRQIERSSRTLGCSWEFIPVDDGWGERPL

Number of residues

1296

Molecular Weight

149424.555

Theoretical pI

Not Available

GO Classification

Functions

metalloendopeptidase activity / zinc ion binding

Processes

inhibition of neurotransmitter uptake / pathogenesis

Components

extracellular region

General Function

Not Available

Specific Function

metalloendopeptidase activity

Pfam Domain Function

• Peptidase_M27 (PF01742)
• Toxin_R_bind_C (PF07951)
• Toxin_R_bind_N (PF07953)
• Toxin_trans (PF07952)

Signal Regions

Not Available

Transmembrane Regions

Not Available

Cellular Location

Not Available

Gene sequence

Not Available

Chromosome Location

Not Available

Locus

Not Available

External Identifiers

Resource	Link
UniProtKB ID	Q7B8V4
UniProtKB Entry Name	Q7B8V4_CLOBO
PDB ID(s)	2ISE, 2ISG, 2ISH, 3NF3

General References

1. Burnett JC, Ruthel G, Stegmann CM, Panchal RG, Nguyen TL, Hermone AR, Stafford RG, Lane DJ, Kenny TA, McGrath CF, Wipf P, Stahl AM, Schmidt JJ, Gussio R, Brunger AT, Bavari S: Inhibition of metalloprotease botulinum serotype A from a pseudo-peptide binding mode to a small molecule that is active in primary neurons. J Biol Chem. 2007 Feb 16;282(7):5004-14. Epub 2006 Nov 8. [Article]
2. Zuniga JE, Hammill JT, Drory O, Nuss JE, Burnett JC, Gussio R, Wipf P, Bavari S, Brunger AT: Iterative structure-based peptide-like inhibitor design against the botulinum neurotoxin serotype A. PLoS One. 2010 Jun 30;5(6):e11378. doi: 10.1371/journal.pone.0011378. [Article]

Associated Data

Drug Relations

Drug	Drug group	Pharmacological action?	Type	Actions	Details
(2E)-3-(2,4-DICHLOROPHENYL)-N-HYDROXYACRYLAMIDE	experimental	unknown	target		Details
(2E)-3-(4-CHLOROPHENYL)-N-HYDROXYACRYLAMIDE	experimental	unknown	target		Details
Equine Botulinum Neurotoxin A Immune FAB2	approved, experimental, investigational	yes	target	antibody	Details