N-acetylmuramoyl-L-alanine amidase

Details

Name
N-acetylmuramoyl-L-alanine amidase
Kind
protein
Synonyms
  • 3.5.1.28
  • Peptidoglycan recognition protein 2
  • Peptidoglycan recognition protein long
  • PGLYRPL
  • PGRP-L
  • PGRPL
Gene Name
PGLYRP2
UniProtKB Entry
Q96PD5Swiss-Prot
Organism
Humans
NCBI Taxonomy ID
9606
Amino acid sequence
>lcl|BSEQ0009532|N-acetylmuramoyl-L-alanine amidase
MAQGVLWILLGLLLWSDPGTASLPLLMDSVIQALAELEQKVPAAKTRHTASAWLMSAPNS
GPHNRLYHFLLGAWSLNATELDPCPLSPELLGLTKEVARHDVREGKEYGVVLAPDGSTVA
VEPLLAGLEAGLQGRRVINLPLDSMAAPWETGDTFPDVVAIAPDVRATSSPGLRDGSPDV
TTADIGANTPDATKGCPDVQASLPDAKAKSPPTMVDSLLAVTLAGNLGLTFLRGSQTQSH
PDLGTEGCWDQLSAPRTFTLLDPKASLLTMAFLNGALDGVILGDYLSRTPEPRPSLSHLL
SQYYGAGVARDPGFRSNFRRQNGAALTSASILAQQVWGTLVLLQRLEPVHLQLQCMSQEQ
LAQVAANATKEFTEAFLGCPAIHPRCRWGAAPYRGRPKLLQLPLGFLYVHHTYVPAPPCT
DFTRCAANMRSMQRYHQDTQGWGDIGYSFVVGSDGYVYEGRGWHWVGAHTLGHNSRGFGV
AIVGNYTAALPTEAALRTVRDTLPSCAVRAGLLRPDYALLGHRQLVRTDCPGDALFDLLR
TWPHFTATVKPRPARSVSKRSRREPPPRTLPATDLQ
Number of residues
576
Molecular Weight
62216.365
Theoretical pI
Not Available
GO Classification
Functions
N-acetylmuramoyl-L-alanine amidase activity / peptidoglycan binding / zinc ion binding
Processes
defense response to Gram-positive bacterium / detection of bacterium / innate immune response / negative regulation of natural killer cell differentiation involved in immune response / peptide amidation / peptidoglycan catabolic process / regulation of inflammatory response
Components
extracellular exosome / membrane
General Function
May play a scavenger role by digesting biologically active peptidoglycan (PGN) into biologically inactive fragments. Has no direct bacteriolytic activity
Specific Function
N-acetylmuramoyl-l-alanine amidase activity
Pfam Domain Function
Signal Regions
1-21
Transmembrane Regions
Not Available
Cellular Location
Secreted
Gene sequence
>lcl|BSEQ0021390|N-acetylmuramoyl-L-alanine amidase (PGLYRP2)
ATGGCCCAGGGTGTCCTCTGGATCCTACTCGGATTGCTACTGTGGTCAGACCCAGGGACA
GCCTCCCTGCCCCTGCTCATGGACTCTGTCATCCAGGCCCTGGCTGAGCTGGAGCAGAAA
GTGCCAGCTGCCAAGACCAGACACACAGCTTCTGCGTGGCTGATGTCAGCTCCAAACTCT
GGCCCCCACAATCGCCTCTACCACTTCCTGCTGGGGGCATGGAGCCTCAATGCTACAGAG
TTGGATCCCTGCCCACTAAGCCCAGAGCTGTTAGGCCTGACCAAGGAGGTGGCCCGACAT
GACGTACGAGAAGGGAAGGAATATGGGGTGGTGCTGGCACCTGATGGCTCGACCGTGGCT
GTGGAGCCTCTGCTGGCGGGGCTGGAGGCAGGGCTGCAAGGGCGCAGGGTCATAAATTTG
CCCTTGGACAGCATGGCTGCCCCTTGGGAGACTGGAGATACCTTTCCAGATGTTGTGGCC
ATTGCTCCAGATGTAAGAGCCACCTCCTCCCCAGGACTCAGGGATGGCTCTCCAGATGTC
ACCACTGCAGATATTGGAGCCAACACTCCAGATGCTACAAAAGGCTGTCCAGATGTCCAA
GCTTCCTTGCCAGATGCCAAAGCCAAGTCCCCACCGACCATGGTGGACAGCCTCCTGGCA
GTCACCCTGGCTGGAAACCTGGGCCTGACCTTCCTCCGAGGTTCCCAGACCCAGAGCCAT
CCAGACCTGGGAACTGAGGGCTGCTGGGACCAGCTCTCTGCCCCTCGGACCTTTACGCTT
TTGGACCCCAAGGCATCTCTGTTAACCATGGCCTTCCTCAATGGCGCCCTGGATGGGGTC
ATCCTTGGAGACTACCTGAGCCGGACTCCTGAGCCCCGGCCATCCCTCAGCCACTTGCTG
AGCCAGTACTATGGGGCTGGGGTGGCCAGAGACCCAGGGTTCCGCAGCAACTTCCGACGG
CAGAACGGTGCTGCTCTGACTTCAGCCTCCATCCTGGCCCAGCAGGTGTGGGGAACCCTT
GTCCTTCTACAGAGGCTGGAGCCAGTACACCTCCAGCTTCAGTGCATGAGCCAAGAACAG
CTGGCCCAGGTGGCTGCCAATGCTACCAAGGAATTCACTGAGGCCTTCCTGGGATGCCCG
GCCATCCACCCCCGCTGCCGCTGGGGAGCGGCGCCTTATCGGGGCCGCCCGAAGCTGCTG
CAGCTGCCGCTGGGATTCTTGTACGTGCATCACACCTACGTGCCTGCACCACCCTGCACG
GACTTCACGCGCTGCGCAGCCAACATGCGCTCCATGCAGCGCTACCACCAGGACACGCAA
GGCTGGGGAGACATCGGCTACAGTTTCGTGGTGGGCTCGGACGGCTACGTGTACGAGGGA
CGCGGCTGGCACTGGGTGGGCGCCCACACGCTCGGCCACAACTCCCGGGGCTTCGGCGTG
GCCATAGTGGGCAACTACACCGCGGCGCTGCCCACCGAGGCCGCTCTGCGCACGGTGCGC
GACACGCTCCCGAGTTGTGCGGTGCGCGCCGGCCTCCTGCGGCCAGACTACGCGCTGCTG
GGCCACCGCCAGCTGGTGCGCACCGACTGCCCCGGCGACGCGCTCTTCGACCTGCTGCGC
ACCTGGCCGCACTTCACCGCGACTGTTAAGCCAAGACCTGCCAGGAGTGTCTCTAAGAGA
TCCAGGAGGGAGCCACCCCCAAGGACCCTGCCAGCCACAGACCTCCAATAA
Chromosome Location
19
Locus
19p13.12
External Identifiers
ResourceLink
UniProtKB IDQ96PD5
UniProtKB Entry NamePGRP2_HUMAN
GeneCard IDPGLYRP2
HGNC IDHGNC:30013
KEGG IDhsa:114770
NCBI Gene ID114770
General References
  1. Liu C, Xu Z, Gupta D, Dziarski R: Peptidoglycan recognition proteins: a novel family of four human innate immunity pattern recognition molecules. J Biol Chem. 2001 Sep 14;276(37):34686-94. Epub 2001 Jul 18. [Article]
  2. Yamada S, Ohira M, Horie H, Ando K, Takayasu H, Suzuki Y, Sugano S, Hirata T, Goto T, Matsunaga T, Hiyama E, Hayashi Y, Ando H, Suita S, Kaneko M, Sasaki F, Hashizume K, Ohnuma N, Nakagawara A: Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas. Oncogene. 2004 Aug 5;23(35):5901-11. [Article]
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  5. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
  6. De Pauw P, Neyt C, Vanderwinkel E, Wattiez R, Falmagne P: Characterization of human serum N-acetylmuramyl-L-alanine amidase purified by affinity chromatography. Protein Expr Purif. 1995 Jun;6(3):371-8. [Article]
  7. Zhang Z, Henzel WJ: Signal peptide prediction based on analysis of experimentally verified cleavage sites. Protein Sci. 2004 Oct;13(10):2819-24. Epub 2004 Aug 31. [Article]
  8. Zhang Y, van der Fits L, Voerman JS, Melief MJ, Laman JD, Wang M, Wang H, Wang M, Li X, Walls CD, Gupta D, Dziarski R: Identification of serum N-acetylmuramoyl-l-alanine amidase as liver peptidoglycan recognition protein 2. Biochim Biophys Acta. 2005 Aug 31;1752(1):34-46. [Article]
  9. Wang ZM, Li X, Cocklin RR, Wang M, Wang M, Fukase K, Inamura S, Kusumoto S, Gupta D, Dziarski R: Human peptidoglycan recognition protein-L is an N-acetylmuramoyl-L-alanine amidase. J Biol Chem. 2003 Dec 5;278(49):49044-52. Epub 2003 Sep 23. [Article]
  10. Zhang H, Li XJ, Martin DB, Aebersold R: Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry. Nat Biotechnol. 2003 Jun;21(6):660-6. Epub 2003 May 18. [Article]
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Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
Copperapproved, investigationalunknowntargetDetails
Zincapproved, investigationalunknowntargetDetails
Zinc acetateapproved, investigationalunknowntargetDetails
Zinc chlorideapproved, investigationalunknowntargetbinderDetails
Zinc sulfate, unspecified formapproved, experimentalunknowntargetbinderDetails