DNA topoisomerase 4 subunit B

Details

Name
DNA topoisomerase 4 subunit B
Kind
protein
Synonyms
  • 5.99.1.3
  • grlB
  • Topoisomerase IV subunit B
Gene Name
parE
UniProtKB Entry
Q59192Swiss-Prot
Organism
Bacillus subtilis (strain 168)
NCBI Taxonomy ID
224308
Amino acid sequence
>lcl|BSEQ0037657|DNA topoisomerase 4 subunit B
MARKQQFDYNEDAIQVLEGLEAVRKRPGMYIGSTDARGLHHLVYEIVDNSVDEVLAGHGD
HIIVKIHKDNSISVQDRGRGMPTGMHKLGKPTPEVILTVLHAGGKFGQGGYKTSGGLHGV
GASVVNALSEWLTVTIERDGFVYQQRFENGGKPVTSLEKIGKTKKTGTLTHFKPDPTMFS
TTTYNFETLSERLRESAFLLKGLKIELIDERNDQREVFYYENGIEAFVAYLNEEKDVLSE
VVSFEGEHHSIEVDFAFQFNDGYSENILSFVNNVRTKDGGTHESGAKTAMTRAFNEYARK
VALLKEKDKNLEGTDIREGLSAIISVRIPEELLQFEGQTKGKLGTSEARSAVDAIVSEQL
AYFLEENRDTATLLVKKAIKASQAREAARKAREEARSGKKRKKSEATLSGKLTPAGSRNP
AKNELYLVEGDSAGGSAKQGRDRRFQAVLPLRGKVINTEKAKLADIFKNEEINTIIHAIG
GGVGADFSIDDINYDKIIIMTDADTDGAHIQVLLLTFFYRYMKPLIEHGKVFIALPPLYK
VSKGSGKKEIIEYAWSDEEMGDVLKKVGKGYTIQRYKGLGEMNADQLWETTMNPESRTLV
RVKIDDAARVERRVTTLMGDKVEPRRKWIEKNVAFGLDEESNILENENLSVAEEV
Number of residues
655
Molecular Weight
73006.17
Theoretical pI
Not Available
GO Classification
Functions
ATP binding / DNA binding / DNA topoisomerase type II (ATP-hydrolyzing) activity / magnesium ion binding
Processes
chromosome segregation / DNA topological change / DNA unwinding involved in DNA replication
Components
chromosome / DNA topoisomerase complex (ATP-hydrolyzing) / nucleoid
General Function
Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.
Specific Function
ATP binding
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0015839|DNA topoisomerase 4 subunit B (parE)
TTGGCTAGAAAACAGCAATTTGATTACAACGAAGATGCCATACAGGTGCTCGAAGGCCTT
GAGGCTGTCAGGAAAAGGCCGGGTATGTATATTGGCTCTACTGACGCGCGGGGTTTGCAC
CACTTGGTTTATGAAATTGTAGATAACTCTGTCGACGAGGTGCTTGCCGGCCATGGAGAT
CACATAATCGTCAAAATACATAAAGACAATAGTATTTCTGTACAGGATAGAGGCCGCGGC
ATGCCGACCGGAATGCATAAGCTCGGCAAACCAACCCCTGAAGTGATTTTAACGGTGCTT
CATGCCGGAGGGAAATTCGGCCAAGGCGGCTATAAGACAAGCGGCGGGCTCCACGGAGTT
GGTGCATCAGTCGTGAATGCTTTATCTGAATGGCTGACGGTTACCATTGAGCGGGACGGT
TTTGTCTATCAGCAGCGATTTGAAAATGGAGGAAAACCGGTTACATCTCTCGAAAAGATC
GGCAAAACAAAAAAGACGGGAACTCTTACACACTTTAAGCCAGATCCAACGATGTTCAGC
ACAACCACTTATAATTTTGAAACGCTCTCTGAGCGCTTAAGAGAGTCTGCGTTTTTGTTA
AAAGGACTGAAAATTGAGCTGATTGATGAACGCAATGACCAGCGTGAGGTCTTTTATTAT
GAAAACGGCATAGAAGCGTTTGTTGCTTACTTAAATGAAGAAAAAGACGTCTTGTCTGAA
GTGGTCTCGTTTGAGGGTGAGCATCATTCTATAGAAGTGGACTTTGCGTTCCAGTTTAAC
GATGGTTATTCTGAAAATATTTTGTCGTTTGTCAACAATGTCAGAACAAAAGACGGCGGG
ACGCATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAA
GTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTT
TCAGCCATTATTTCTGTCCGCATTCCTGAGGAGCTTCTTCAATTTGAGGGACAAACAAAA
GGAAAGCTCGGTACCAGTGAAGCAAGATCAGCGGTGGATGCGATTGTTTCAGAGCAGCTT
GCTTACTTTTTAGAGGAAAATCGGGACACCGCAACGCTTCTTGTCAAAAAAGCAATCAAG
GCGAGCCAGGCGCGCGAAGCGGCACGCAAAGCCCGTGAAGAAGCAAGAAGCGGCAAAAAA
CGAAAAAAATCAGAAGCGACTTTAAGCGGAAAGCTGACGCCTGCGGGTTCAAGAAACCCG
GCAAAAAATGAATTGTATCTCGTTGAGGGAGATTCAGCAGGCGGGTCAGCCAAGCAGGGA
CGAGACCGCAGATTCCAGGCGGTTCTGCCTTTACGCGGTAAAGTCATTAATACAGAAAAA
GCTAAGCTTGCTGATATCTTTAAAAATGAAGAGATCAATACGATCATTCATGCGATCGGC
GGGGGAGTCGGGGCAGATTTCTCAATCGATGACATCAACTACGACAAAATCATCATTATG
ACCGATGCCGATACTGACGGCGCCCATATTCAAGTGCTGCTTCTTACCTTTTTCTATCGC
TATATGAAACCGCTCATTGAGCATGGCAAGGTGTTCATTGCGCTGCCACCTTTATATAAG
GTCAGTAAAGGAAGCGGGAAAAAGGAAATCATTGAATATGCATGGTCTGATGAAGAGATG
GGGGATGTCCTGAAAAAAGTCGGCAAAGGCTATACCATTCAACGATATAAGGGTCTTGGA
GAGATGAACGCAGACCAGCTGTGGGAAACGACGATGAATCCGGAGTCCCGCACACTTGTT
AGGGTCAAAATTGACGATGCGGCACGTGTAGAGAGACGTGTGACGACGTTAATGGGAGAC
AAAGTAGAGCCGCGCAGAAAGTGGATTGAGAAAAACGTCGCTTTCGGCTTAGATGAAGAA
AGCAATATTTTAGAAAATGAAAACTTATCGGTCGCTGAGGAGGTTTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDQ59192
UniProtKB Entry NamePARE_BACSU
KEGG IDbsu:BSU18090
NCBI Gene ID936151
General References
  1. Rose M, Entian KD: New genes in the 170 degrees region of the Bacillus subtilis genome encode DNA gyrase subunits, a thioredoxin, a xylanase and an amino acid transporter. Microbiology. 1996 Nov;142 ( Pt 11):3097-101. [Article]
  2. Huang WM, Libbey JL, van der Hoeven P, Yu SX: Bipolar localization of Bacillus subtilis topoisomerase IV, an enzyme required for chromosome segregation. Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4652-7. [Article]
  3. Kunst F, Ogasawara N, Moszer I, Albertini AM, Alloni G, Azevedo V, Bertero MG, Bessieres P, Bolotin A, Borchert S, Borriss R, Boursier L, Brans A, Braun M, Brignell SC, Bron S, Brouillet S, Bruschi CV, Caldwell B, Capuano V, Carter NM, Choi SK, Cordani JJ, Connerton IF, Cummings NJ, Daniel RA, Denziot F, Devine KM, Dusterhoft A, Ehrlich SD, Emmerson PT, Entian KD, Errington J, Fabret C, Ferrari E, Foulger D, Fritz C, Fujita M, Fujita Y, Fuma S, Galizzi A, Galleron N, Ghim SY, Glaser P, Goffeau A, Golightly EJ, Grandi G, Guiseppi G, Guy BJ, Haga K, Haiech J, Harwood CR, Henaut A, Hilbert H, Holsappel S, Hosono S, Hullo MF, Itaya M, Jones L, Joris B, Karamata D, Kasahara Y, Klaerr-Blanchard M, Klein C, Kobayashi Y, Koetter P, Koningstein G, Krogh S, Kumano M, Kurita K, Lapidus A, Lardinois S, Lauber J, Lazarevic V, Lee SM, Levine A, Liu H, Masuda S, Mauel C, Medigue C, Medina N, Mellado RP, Mizuno M, Moestl D, Nakai S, Noback M, Noone D, O'Reilly M, Ogawa K, Ogiwara A, Oudega B, Park SH, Parro V, Pohl TM, Portelle D, Porwollik S, Prescott AM, Presecan E, Pujic P, Purnelle B, Rapoport G, Rey M, Reynolds S, Rieger M, Rivolta C, Rocha E, Roche B, Rose M, Sadaie Y, Sato T, Scanlan E, Schleich S, Schroeter R, Scoffone F, Sekiguchi J, Sekowska A, Seror SJ, Serror P, Shin BS, Soldo B, Sorokin A, Tacconi E, Takagi T, Takahashi H, Takemaru K, Takeuchi M, Tamakoshi A, Tanaka T, Terpstra P, Togoni A, Tosato V, Uchiyama S, Vandebol M, Vannier F, Vassarotti A, Viari A, Wambutt R, Wedler H, Weitzenegger T, Winters P, Wipat A, Yamamoto H, Yamane K, Yasumoto K, Yata K, Yoshida K, Yoshikawa HF, Zumstein E, Yoshikawa H, Danchin A: The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature. 1997 Nov 20;390(6657):249-56. [Article]

Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
Ciprofloxacinapproved, investigationalunknowntargetDetails