Leucyl-cystinyl aminopeptidase

Details

Name
Leucyl-cystinyl aminopeptidase
Kind
protein
Synonyms
  • 3.4.11.3
  • Cystinyl aminopeptidase
  • Insulin-regulated membrane aminopeptidase
  • Insulin-responsive aminopeptidase
  • IRAP
  • OTASE
  • Oxytocinase
  • P-LAP
  • Placental leucine aminopeptidase
Gene Name
LNPEP
UniProtKB Entry
Q9UIQ6Swiss-Prot
Organism
Humans
NCBI Taxonomy ID
9606
Amino acid sequence
>lcl|BSEQ0052528|Leucyl-cystinyl aminopeptidase
MEPFTNDRLQLPRNMIENSMFEEEPDVVDLAKEPCLHPLEPDEVEYEPRGSRLLVRGLGE
HEMEEDEEDYESSAKLLGMSFMNRSSGLRNSATGYRQSPDGACSVPSARTMVVCAFVIVV
AVSVIMVIYLLPRCTFTKEGCHKKNQSIGLIQPFATNGKLFPWAQIRLPTAVVPLRYELS
LHPNLTSMTFRGSVTISVQALQVTWNIILHSTGHNISRVTFMSAVSSQEKQAEILEYAYH
GQIAIVAPEALLAGHNYTLKIEYSANISSSYYGFYGFSYTDESNEKKYFAATQFEPLAAR
SAFPCFDEPAFKATFIIKIIRDEQYTALSNMPKKSSVVLDDGLVQDEFSESVKMSTYLVA
FIVGEMKNLSQDVNGTLVSIYAVPEKIGQVHYALETTVKLLEFFQNYFEIQYPLKKLDLV
AIPDFEAGAMENWGLLTFREETLLYDSNTSSMADRKLVTKIIAHELAHQWFGNLVTMKWW
NDLWLNEGFATFMEYFSLEKIFKELSSYEDFLDARFKTMKKDSLNSSHPISSSVQSSEQI
EEMFDSLSYFKGSSLLLMLKTYLSEDVFQHAVVLYLHNHSYASIQSDDLWDSFNEVTNQT
LDVKRMMKTWTLQKGFPLVTVQKKGKELFIQQERFFLNMKPEIQPSDTSYLWHIPLSYVT
EGRNYSKYQSVSLLDKKSGVINLTEEVLWVKVNINMNGYYIVHYADDDWEALIHQLKINP
YVLSDKDRANLINNIFELAGLGKVPLKRAFDLINYLGNENHTAPITEALFQTDLIYNLLE
KLGYMDLASRLVTRVFKLLQNQIQQQTWTDEGTPSMRELRSALLEFACTHNLGNCSTTAM
KLFDDWMASNGTQSLPTDVMTTVFKVGAKTDKGWSFLLGKYISIGSEAEKNKILEALASS
EDVRKLYWLMKSSLNGDNFRTQKLSFIIRTVGRHFPGHLLAWDFVKENWNKLVQKFPLGS
YTIQNIVAGSTYLFSTKTHLSEVQAFFENQSEATFRLRCVQEALEVIQLNIQWMEKNLKS
LTWWL
Number of residues
1025
Molecular Weight
117348.14
Theoretical pI
Not Available
GO Classification
Components
extracellular space
General Function
Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain
Specific Function
aminopeptidase activity
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
111-131
Cellular Location
Cell membrane
Gene sequence
>lcl|BSEQ0052529|Leucyl-cystinyl aminopeptidase (LNPEP)
ATGGAGCCCTTCACCAATGATCGGCTTCAGCTCCCCAGGAATATGATTGAAAACAGCATG
TTTGAGGAAGAACCAGATGTGGTGGATTTAGCCAAAGAGCCTTGTTTACATCCTCTAGAG
CCTGATGAGGTGGAATATGAGCCCCGGGGTTCCCGACTGCTGGTGCGGGGTCTTGGTGAG
CATGAGATGGAGGAGGATGAAGAGGATTATGAGTCATCAGCAAAGCTGCTGGGCATGTCC
TTCATGAATAGAAGCTCAGGCCTTCGGAACAGTGCAACTGGTTACAGGCAGAGCCCAGAT
GGGGCTTGTTCAGTACCCTCTGCAAGGACCATGGTGGTCTGTGCTTTTGTCATCGTGGTT
GCTGTTTCTGTAATCATGGTGATTTACTTACTGCCCAGATGTACCTTTACCAAAGAAGGC
TGCCATAAAAAAAACCAGTCAATTGGACTAATTCAGCCATTTGCAACAAATGGGAAATTG
TTTCCATGGGCACAGATCAGGCTTCCCACTGCCGTTGTGCCACTACGCTATGAACTCAGC
CTACACCCGAACCTAACCTCGATGACATTCAGGGGTTCTGTGACAATTTCAGTTCAGGCT
CTTCAGGTCACATGGAATATCATTCTTCATAGCACAGGTCATAATATTTCAAGAGTGACC
TTTATGTCAGCAGTTTCAAGCCAAGAAAAACAAGCTGAGATCCTGGAATATGCATATCAT
GGACAGATCGCCATTGTTGCCCCCGAAGCCCTTCTAGCAGGGCACAATTATACGTTGAAG
ATAGAGTACTCGGCAAATATATCTAGTTCTTATTATGGGTTTTATGGCTTCTCCTACACA
GATGAAAGTAATGAGAAAAAGTACTTTGCAGCAACTCAGTTTGAACCCCTGGCAGCAAGA
TCTGCTTTTCCTTGTTTTGATGAACCAGCATTTAAAGCCACTTTTATCATCAAGATCATA
AGGGATGAGCAATACACCGCTTTATCAAATATGCCTAAGAAGTCATCAGTCGTTCTAGAT
GATGGACTTGTTCAGGATGAGTTTTCTGAGAGTGTGAAGATGAGCACTTACTTGGTTGCT
TTCATTGTGGGAGAGATGAAGAACCTGAGTCAGGACGTAAATGGAACCCTGGTTTCTATA
TATGCTGTACCAGAAAAGATTGGTCAAGTTCATTATGCCTTGGAAACAACTGTGAAGCTT
CTTGAGTTTTTTCAAAACTACTTTGAAATTCAGTACCCACTTAAGAAATTGGATTTGGTG
GCTATTCCTGACTTTGAAGCAGGAGCAATGGAAAATTGGGGTTTGCTCACCTTCCGAGAG
GAGACACTTCTGTATGACAGTAACACTTCTTCAATGGCGGATAGAAAGCTGGTGACTAAA
ATCATTGCTCATGAGCTGGCCCACCAGTGGTTTGGCAATCTGGTAACAATGAAGTGGTGG
AATGACCTATGGCTAAATGAAGGTTTTGCCACTTTCATGGAGTATTTCTCTTTGGAAAAA
ATATTCAAAGAGCTTTCTAGTTATGAAGATTTCTTAGATGCTCGATTTAAAACCATGAAG
AAAGATTCCTTAAATTCATCTCATCCAATATCATCATCTGTTCAGTCTTCAGAACAAATT
GAAGAAATGTTTGATTCTCTTTCCTATTTTAAGGGATCTTCTCTCTTGTTGATGTTGAAA
ACTTACCTTAGTGAAGATGTGTTTCAACATGCTGTTGTCCTTTACCTGCATAATCACAGC
TATGCATCTATTCAAAGTGATGATCTGTGGGATAGTTTTAATGAGGTCACAAACCAAACA
CTAGATGTAAAGAGAATGATGAAAACCTGGACCCTGCAGAAAGGATTTCCTTTAGTGACT
GTTCAAAAGAAAGGAAAGGAACTTTTTATACAACAAGAGAGATTCTTTTTAAATATGAAG
CCTGAAATTCAGCCTTCAGATACAAGCTACCTGTGGCATATTCCACTATCCTATGTCACT
GAAGGAAGAAATTATTCAAAATATCAATCGGTATCATTACTGGATAAGAAATCAGGTGTC
ATCAATCTTACAGAAGAAGTGCTGTGGGTCAAAGTGAATATAAACATGAATGGTTATTAT
ATTGTACACTATGCAGATGATGATTGGGAAGCACTAATCCATCAGTTGAAAATAAATCCT
TATGTTCTGAGTGACAAAGACCGAGCCAACCTTATCAACAACATCTTTGAACTTGCAGGC
CTAGGCAAGGTACCTCTCAAGAGGGCCTTTGATTTGATTAATTATCTTGGAAATGAGAAC
CATACTGCACCCATCACCGAAGCCCTGTTTCAGACAGACCTCATCTATAACCTCCTTGAA
AAACTGGGATACATGGATCTGGCCTCAAGACTGGTGACTAGGGTATTTAAATTACTTCAA
AACCAAATTCAACAACAAACTTGGACTGATGAGGGCACTCCATCTATGCGAGAGCTTCGG
TCAGCCCTGCTAGAGTTTGCTTGCACCCACAACCTGGGGAACTGCTCTACTACTGCCATG
AAACTGTTTGATGACTGGATGGCATCCAATGGAACTCAAAGCCTACCTACTGATGTCATG
ACAACTGTGTTCAAAGTTGGAGCAAAAACTGACAAAGGCTGGTCATTCCTTTTGGGCAAA
TACATTTCTATAGGCTCTGAAGCAGAGAAGAACAAAATACTAGAAGCACTTGCCAGCTCA
GAGGATGTGCGGAAGCTTTACTGGTTAATGAAAAGTAGCCTGAATGGAGATAACTTCCGA
ACACAGAAGCTGTCTTTTATCATTAGAACAGTGGGTCGACATTTTCCTGGACACTTACTG
GCATGGGATTTTGTCAAAGAGAACTGGAATAAGCTTGTACAGAAGTTCCCTCTGGGGTCC
TATACCATACAAAATATTGTTGCTGGATCAACTTACCTGTTTTCAACAAAGACACATTTA
TCTGAGGTTCAGGCATTCTTTGAAAATCAGTCAGAGGCAACCTTCCGGCTTCGTTGTGTC
CAGGAGGCTTTGGAAGTCATTCAGTTGAATATCCAGTGGATGGAGAAGAACCTCAAAAGT
CTCACATGGTGGCTGTAG
Chromosome Location
5
Locus
5q15
External Identifiers
ResourceLink
UniProtKB IDQ9UIQ6
UniProtKB Entry NameLCAP_HUMAN
GeneCard IDLNPEP
HGNC IDHGNC:6656
PDB ID(s)4P8Q, 4PJ6, 4Z7I, 5C97, 5JHQ, 5MJ6, 7ZYF, 8CGP, 8CGW
KEGG IDhsa:4012
IUPHAR/Guide To Pharmacology ID1570
NCBI Gene ID4012
General References
  1. Rogi T, Tsujimoto M, Nakazato H, Mizutani S, Tomoda Y: Human placental leucine aminopeptidase/oxytocinase. A new member of type II membrane-spanning zinc metallopeptidase family. J Biol Chem. 1996 Jan 5;271(1):56-61. doi: 10.1074/jbc.271.1.56. [Article]
  2. Laustsen PG, Rasmussen TE, Petersen K, Pedraza-Diaz S, Moestrup SK, Gliemann J, Sottrup-Jensen L, Kristensen T: The complete amino acid sequence of human placental oxytocinase. Biochim Biophys Acta. 1997 May 2;1352(1):1-7. doi: 10.1016/s0167-4781(97)00036-5. [Article]
  3. Rasmussen TE, Pedraza-Diaz S, Hardre R, Laustsen PG, Carrion AG, Kristensen T: Structure of the human oxytocinase/insulin-regulated aminopeptidase gene and localization to chromosome 5q21. Eur J Biochem. 2000 Apr;267(8):2297-306. doi: 10.1046/j.1432-1327.2000.01234.x. [Article]
  4. Matsumoto H, Nagasaka T, Hattori A, Rogi T, Tsuruoka N, Mizutani S, Tsujimoto M: Expression of placental leucine aminopeptidase/oxytocinase in neuronal cells and its action on neuronal peptides. Eur J Biochem. 2001 Jun;268(11):3259-66. doi: 10.1046/j.1432-1327.2001.02221.x. [Article]
  5. Falk K, Rotzschke O, Stevanovic S, Jung G, Rammensee HG: Pool sequencing of natural HLA-DR, DQ, and DP ligands reveals detailed peptide motifs, constraints of processing, and general rules. Immunogenetics. 1994;39(4):230-42. doi: 10.1007/BF00188785. [Article]
  6. Tsujimoto M, Mizutani S, Adachi H, Kimura M, Nakazato H, Tomoda Y: Identification of human placental leucine aminopeptidase as oxytocinase. Arch Biochem Biophys. 1992 Feb 1;292(2):388-92. doi: 10.1016/0003-9861(92)90007-j. [Article]
  7. Albiston AL, McDowall SG, Matsacos D, Sim P, Clune E, Mustafa T, Lee J, Mendelsohn FA, Simpson RJ, Connolly LM, Chai SY: Evidence that the angiotensin IV (AT(4)) receptor is the enzyme insulin-regulated aminopeptidase. J Biol Chem. 2001 Dec 28;276(52):48623-6. doi: 10.1074/jbc.C100512200. Epub 2001 Nov 13. [Article]
  8. Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31. [Article]
  9. Wollscheid B, Bausch-Fluck D, Henderson C, O'Brien R, Bibel M, Schiess R, Aebersold R, Watts JD: Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins. Nat Biotechnol. 2009 Apr;27(4):378-86. doi: 10.1038/nbt.1532. Epub 2009 Apr 6. [Article]
  10. Burkard TR, Planyavsky M, Kaupe I, Breitwieser FP, Burckstummer T, Bennett KL, Superti-Furga G, Colinge J: Initial characterization of the human central proteome. BMC Syst Biol. 2011 Jan 26;5:17. doi: 10.1186/1752-0509-5-17. [Article]
  11. Van Damme P, Lasa M, Polevoda B, Gazquez C, Elosegui-Artola A, Kim DS, De Juan-Pardo E, Demeyer K, Hole K, Larrea E, Timmerman E, Prieto J, Arnesen T, Sherman F, Gevaert K, Aldabe R: N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12449-54. doi: 10.1073/pnas.1210303109. Epub 2012 Jul 18. [Article]
  12. Zhou H, Di Palma S, Preisinger C, Peng M, Polat AN, Heck AJ, Mohammed S: Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res. 2013 Jan 4;12(1):260-71. doi: 10.1021/pr300630k. Epub 2012 Dec 18. [Article]
  13. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. [Article]
  14. Vaca Jacome AS, Rabilloud T, Schaeffer-Reiss C, Rompais M, Ayoub D, Lane L, Bairoch A, Van Dorsselaer A, Carapito C: N-terminome analysis of the human mitochondrial proteome. Proteomics. 2015 Jul;15(14):2519-24. doi: 10.1002/pmic.201400617. Epub 2015 Jun 8. [Article]

Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
Oxytocinapproved, vet_approvedunknownenzymesubstrateDetails