In vitro RNA editing in plant mitochondria does not require added energy.

Article Details

Citation

Copy to clipboard

Takenaka M, Verbitskiy D, van der Merwe JA, Zehrmann A, Plessmann U, Urlaub H, Brennicke A

In vitro RNA editing in plant mitochondria does not require added energy.

FEBS Lett. 2007 Jun 12;581(14):2743-7. Epub 2007 May 21.

PubMed ID

17531229 [ View in PubMed

]

Abstract

RNA editing in flowering plant mitochondria is investigated by in vitro assays. These cauliflower mitochondrial lysates require added NTP or dNTP. We have now resolved the reason for this requirement to be the inhibition of the RNA binding activity of the glutamate dehydrogenases (GDH). Both GDH1 and GDH2 were identified in RNA-protein cross-links. The inhibition of in vitro RNA editing by GDH is confirmed by the ability of the GDH-specific herbicide phosphinothricin to substitute for NTP. NADH and NADPH, but not NAD or NADP, can also replace NTP, suggesting that the NAD(P)H-binding-pocket configuration of the GDH contacts the RNA. RNA editing in plant mitochondria is thus intrinsically independent of added energy in the form of NTP.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
NADH	GDH/6PGL endoplasmic bifunctional protein	Protein	Humans	Unknown	Not Available	Details