Characterization of the 5' regulatory region of the human sodium-dependent multivitamin transporter, hSMVT.

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Dey S, Subramanian VS, Chatterjee NS, Rubin SA, Said HM

Characterization of the 5' regulatory region of the human sodium-dependent multivitamin transporter, hSMVT.

Biochim Biophys Acta. 2002 Mar 19;1574(2):187-92.

PubMed ID

11955628 [ View in PubMed

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Abstract

We cloned and functionally characterized the 5' regulatory region of the human sodium-dependent multivitamin transporter (hSMVT) gene, a remarkably versatile carrier responsible for uptake of biotin, pantothenic acid and lipoate. Two potential transcriptional start sites were determined by 5'-RACE and found to be at -4603, and -4303. Two distinct promoters (P1 and P2) were identified. Both putative promoter sequences were TATA-less, CAAT-less, contained highly GC-rich sites, and had multiple putative regulatory cis-elements (e.g., AP1, AP2, C/EBP, SP1, NF1, and GATA). The activities of the putative promoters were confirmed using a firefly luciferase reporter gene assay system following transient transfection into three cultured human cell lines: Caco-2, HEK293, and vascular smooth muscle cells. The minimal region required for basal activity of the hSMVT promoter was also determined by generating a series of deletion constructs and found to be encoded by a sequence between -5846 to -5313 for P1 and between -4417 to -4244 for P2 relative to the translation initiation codon. These results demonstrate the first molecular characterization of the regulatory region of this important human gene.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Lipoic acid	Sodium-dependent multivitamin transporter	Protein	Humans	Unknown	Not Available	Details