BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures.

Article Details

Citation

Wang Y, Cortez D, Yazdi P, Neff N, Elledge SJ, Qin J

BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures.

Genes Dev. 2000 Apr 15;14(8):927-39.

PubMed ID
10783165 [ View in PubMed
]
Abstract

We report the identities of the members of a group of proteins that associate with BRCA1 to form a large complex that we have named BASC (BRCA1-associated genome surveillance complex). This complex includes tumor suppressors and DNA damage repair proteins MSH2, MSH6, MLH1, ATM, BLM, and the RAD50-MRE11-NBS1 protein complex. In addition, DNA replication factor C (RFC), a protein complex that facilitates the loading of PCNA onto DNA, is also part of BASC. We find that BRCA1, the BLM helicase, and the RAD50-MRE11-NBS1 complex colocalize to large nuclear foci that contain PCNA when cells are treated with agents that interfere with DNA synthesis. The association of BRCA1 with MSH2 and MSH6, which are required for transcription-coupled repair, provides a possible explanation for the role of BRCA1 in this pathway. Strikingly, all members of this complex have roles in recognition of abnormal DNA structures or damaged DNA, suggesting that BASC may serve as a sensor for DNA damage. Several of these proteins also have roles in DNA replication-associated repair. Collectively, these results suggest that BRCA1 may function as a coordinator of multiple activities required for maintenance of genomic integrity during the process of DNA replication and point to a central role for BRCA1 in DNA repair.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Mismatch repair endonuclease PMS2P54278Details
Serine-protein kinase ATMQ13315Details