A disorder to order transition accompanies catalysis in retinaldehyde dehydrogenase type II.
Article Details
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Bordelon T, Montegudo SK, Pakhomova S, Oldham ML, Newcomer ME
A disorder to order transition accompanies catalysis in retinaldehyde dehydrogenase type II.
J Biol Chem. 2004 Oct 8;279(41):43085-91. Epub 2004 Aug 7.
- PubMed ID
- 15299009 [ View in PubMed]
- Abstract
Retinaldehyde dehydrogenase II (RalDH2) converts retinal to the transcriptional regulator retinoic acid in the developing embryo. The x-ray structure of the enzyme revealed an important structural difference between this protein and other aldehyde dehydrogenases of the same enzyme superfamily; a 20-amino acid span in the substrate access channel in retinaldehyde dehydrogenase II is disordered, whereas in other aldehyde dehydrogenases this region forms a well defined wall of the substrate access channel. We asked whether this disordered loop might order during the course of catalysis and provide a means for an enzyme that requires a large substrate access channel to restrict access to the catalytic machinery by smaller compounds that might potentially enter the active site and be metabolized. Our experiments, a combination of kinetic, spectroscopic, and crystallographic techniques, suggest that a disorder to order transition is linked to catalytic activity.
DrugBank Data that Cites this Article
- Drug Targets
Drug Target Kind Organism Pharmacological Action Actions Tretinoin Retinal dehydrogenase 2 Protein Humans UnknownSubstrateDetails