Evidence for involvement of HMGB1 protein in human DNA mismatch repair.
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Yuan F, Gu L, Guo S, Wang C, Li GM
Evidence for involvement of HMGB1 protein in human DNA mismatch repair.
J Biol Chem. 2004 May 14;279(20):20935-40. Epub 2004 Mar 9.
- PubMed ID
- 15014079 [ View in PubMed]
- Abstract
Defects in human DNA mismatch repair predispose to cancer, but many components of the pathway have not been identified. We report here the identification and characterization of a novel component required for mismatch repair in human cells. A 30-kDa protein was purified to homogeneity by virtue of its ability to complement a depleted HeLa extract in repair of mismatched heteroduplexes. The complementing activity was identified as HMGB1 (the high mobility group box 1 protein), a non-histone chromatin protein that facilitates protein-protein interactions and recognizes DNA damage. Evidence is also presented that HMGB1 physically interacts with MutSalpha and is required at a step prior to the excision of mispaired nucleotide in mismatch repair.