Structure of the human liver/bone/kidney alkaline phosphatase gene.

Article Details


Weiss MJ, Ray K, Henthorn PS, Lamb B, Kadesch T, Harris H

Structure of the human liver/bone/kidney alkaline phosphatase gene.

J Biol Chem. 1988 Aug 25;263(24):12002-10.

PubMed ID
3165380 [ View in PubMed

In man, there are multiple forms of alkaline phosphatase encoded by at least three homologous genes: placental, intestinal, and liver/bone/kidney. This report describes the characterization of the human liver/bone/kidney alkaline phosphatase locus. The gene appears to exist as a single copy in the haploid genome and is comprised of 12 exons distributed over more than 50 kilobases. In liver, kidney, SAOS-2 human osteosarcoma cells, and cultured fibroblasts, there is a single major start for transcription situated about 25 nucleotides downstream of an A/T-rich motif. The promoter region is extremely G/C-rich, is relatively abundant in the dinucleotide CpG, and contains four copies of the consensus sequence for SP1 binding (GGGCGG). The liver/bone/kidney alkaline phosphatase gene is at least five times larger than the intestinal and placental alkaline phosphatase genes, mainly due to intron size differences. Intron-exon junctions occur at analogous positions in all three genes, but there is an extra non-coding exon at the 5' end of the liver/bone/kidney alkaline phosphatase gene. The relevance of our findings with respect to the evolution of the human alkaline phosphatase multigene family is discussed.

DrugBank Data that Cites this Article

NameUniProt ID
Alkaline phosphatase, tissue-nonspecific isozymeP05186Details