PRK1 is targeted to endosomes by the small GTPase, RhoB.

Article Details


Mellor H, Flynn P, Nobes CD, Hall A, Parker PJ

PRK1 is targeted to endosomes by the small GTPase, RhoB.

J Biol Chem. 1998 Feb 27;273(9):4811-4.

PubMed ID
9478917 [ View in PubMed

RhoB has been shown to be an endosomal GTPase both by immunocytochemistry and electron microscopy, however, its role in endocytosis is unknown. Elucidation of the cellular roles of other members of this superfamily of signaling proteins has come with the identification of their downstream partners. We show here that the recently isolated serine/threonine kinase PRK1 is targeted to the endosomal compartment by RhoB. This is established both through immunofluorescence and cell fractionation. PRK1 is shown to interact with activated RhoB in cells and is localized to endosomes through its Rho-binding HR1 domain. Translocation of PRK1 to the endosomal compartment by RhoB is accompanied by a shift in the electrophoretic mobility of the kinase indicative of an accompanying activation.

DrugBank Data that Cites this Article

NameUniProt ID
Rho-related GTP-binding protein RhoBP62745Details
Serine/threonine-protein kinase N1Q16512Details