Unique and overlapping roles for ZipA and FtsA in septal ring assembly in Escherichia coli.
Article Details
- CitationCopy to clipboard
Pichoff S, Lutkenhaus J
Unique and overlapping roles for ZipA and FtsA in septal ring assembly in Escherichia coli.
EMBO J. 2002 Feb 15;21(4):685-93.
- PubMed ID
- 11847116 [ View in PubMed]
- Abstract
ZipA and FtsA are essential division proteins in Escherichia coli that are recruited to the division site by interaction with FtsZ. Utilizing a newly isolated temperature-sensitive mutation in zipA we have more fully characterized the role of ZipA. We confirmed that ZipA is not required for Z ring formation; however, we found that ZipA, like FtsA, is required for recruitment of FtsK and therefore all downstream division proteins. In the absence of FtsA or ZipA Z rings formed; however, in the absence of both, new Z rings were unable to form and preformed Z rings were destabilized. Consistent with this, we found that an FtsZ mutant unable to interact with both ZipA and FtsA was unable to assemble into Z rings. These results demonstrate that ZipA and FtsA are both required for recruitment of additional division proteins to the Z ring, but either one is capable of supporting formation and stabilization of Z rings.