Identification and sequence determination of the host factor gene for bacteriophage Q beta.
Article Details
- CitationCopy to clipboard
Kajitani M, Ishihama A
Identification and sequence determination of the host factor gene for bacteriophage Q beta.
Nucleic Acids Res. 1991 Mar 11;19(5):1063-6.
- PubMed ID
- 2020545 [ View in PubMed]
- Abstract
The host factor (HF-I) required for phage Q beta RNA-directed synthesis of complementary minus-strand RNA was purified to homogeneity from phage-infected Escherichia coli cells. The hfq gene encoding HF-I was cloned using synthetic probes designed based on the partial amino acid sequence of HF-I, and mapped at 94.8 min on the E. coli chromosome downstream of the miaA gene involved in 2-methylthio-N6-(isopentyl)-adenosine (ms2i6A) tRNA modification. Sequence determination of the cloned hfq gene indicated that HF-I is a small protein of Mr 11,166 consisting of 102 amino acid residues.