Molecular cloning and expression of a novel human cDNA related to the diazepam binding inhibitor.
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Suk K, Kim YH, Hwang DY, Ihm SH, Yoo HJ, Lee MS
Molecular cloning and expression of a novel human cDNA related to the diazepam binding inhibitor.
Biochim Biophys Acta. 1999 May 31;1454(1):126-31.
- PubMed ID
- 10354522 [ View in PubMed]
- Abstract
In order to isolate the unidentified autoantigens in autoimmune diabetes, a human pancreatic islet cDNA library was constructed and screened with the sera from the diabetic patients. From the library screening, one clone (DRS-1) that strongly reacted with the sera was isolated. Subsequent sequence analysis revealed that the clone was a novel cDNA related to the diazepam binding inhibitor. DRS-1 was expressed in most tissues including liver, lung, tonsil, and thymus, in addition to pancreatic islets. DRS-1 was in vitro translated and the recombinant DRS-1 protein was expressed in Escherichia coli and purified. The size of the in vitro translated or bacterially expressed DRS-1 protein was in agreement with the conceptually translated polypeptide of DRS-1 cDNA. Further studies are required to test whether or not DRS-1 is a new autoantigen in autoimmune diabetes.