Identification of the O-linked glycosylation site of the human transferrin receptor.
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Hayes GR, Enns CA, Lucas JJ
Identification of the O-linked glycosylation site of the human transferrin receptor.
Glycobiology. 1992 Aug;2(4):355-9.
- PubMed ID
- 1421757 [ View in PubMed]
- Abstract
The human transferrin receptor is a glycoprotein containing three N-linked and one O-linked glycosylation sites. Tryptic digestion of the receptor, followed by chromatography on BioGel P-2 and reverse-phase HPLC, yields a glycopeptide (amino acids 101-120) containing the O-linked site. Amino acid sequence analysis reveals that the site of O-glycosylation is Thr-104. Mass spectral analysis is consistent with the presence of a Gal-GalNAc core with predominantly two sialic acid residues.