Primary structure of the cAMP-dependent phosphorylation site of the plasma membrane calcium pump.
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James PH, Pruschy M, Vorherr TE, Penniston JT, Carafoli E
Primary structure of the cAMP-dependent phosphorylation site of the plasma membrane calcium pump.
Biochemistry. 1989 May 16;28(10):4253-8.
- PubMed ID
- 2548572 [ View in PubMed]
- Abstract
The primary structure of a region of the erythrocyte plasma membrane calcium pump which is phosphorylated by the cAMP-dependent protein kinase has been determined. The sequence is A-P-T-K-R-N-S-S(P)-P-P-P-S-P-D. The site is located between the calmodulin binding domain and the C-terminus of the ATPase. The ATPase is phosphorylated only at this site by the cAMP-dependent protein kinase, and the phosphorylation is inhibited by calmodulin. The effect of the phosphorylation is to decrease the Km for Ca2+ of the purified ATPase from about 10 microM to about 1.4 microM and to increase the Vmax of ATP hydrolysis about 2-fold.