Interaction of antibiotics with A- and P-site-specific bases in 16S ribosomal RNA.

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Woodcock J, Moazed D, Cannon M, Davies J, Noller HF

Interaction of antibiotics with A- and P-site-specific bases in 16S ribosomal RNA.

EMBO J. 1991 Oct;10(10):3099-103.

PubMed ID

1915283 [ View in PubMed

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Abstract

We have studied the interactions of the antibiotics apramycin, kasugamycin, myomycin, neamine and pactamycin with 16S rRNA by chemical probing of drug-ribosome complexes. Kasugamycin and pactamycin, which are believed to affect translational initiation, protect bases in common with P-site-bound tRNA. While kasugamycin protects A794 and G926, and causes enhanced reactivity of C795, pactamycin protects G693 and C795. All four of these bases were previously shown to be protected by P-site tRNA or by edeine, another P-site inhibitor. Apramycin and neamine, which both induce miscoding and inhibit translocation, protect A1408, G1419 and G1494, as was also found earlier for neomycin, gentamicin, kanamycin and paromomycin. A1408 and G1494 were previously shown to be protected by A-site tRNA. Surprisingly, myomycin fails to give strong protection of any bases in 16S rRNA, in spite of having an apparently identical target site and mode of action to streptomycin, which protects several bases in the 915 region. Instead, myomycin gives only weak protection of A1408. These results suggest that the binding site(s) of streptomycin and myomycin have yet to be identified.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Apramycin	16S ribosomal RNA	Nucleotide	Enteric bacteria and other eubacteria	Unknown	Not Available	Details