Liquid chromatographic assay for diflorasone diacetate in cream and ointment formulations.

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Shaw MC, Vanderwielen AJ

Liquid chromatographic assay for diflorasone diacetate in cream and ointment formulations.

J Pharm Sci. 1984 Nov;73(11):1606-8.

PubMed ID
6520764 [ View in PubMed
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Abstract

A high-performance liquid chromatographic assay for quantitating diflorasone diacetate (I) in cream and ointment formulations and in bulk form is described. Isoflupredone acetate (II) was used as the internal standard and a 3-micron silica gel column with a mobile phase comprised of water-saturated butyl chloride-water-saturated methylene chloride-tetrahydrofuran-acetic acid (350:125:10:15) led to an efficient separation. The method gave accurate results for four formulations, two creams and two ointments, as well as the bulk drug. The assay has an RSD of approximately 1.8% for the cream formulations, 1.3% for the ointment formulations, and 1.0% for the bulk drug. The method is specific for I and capable of resolving structurally related compounds.

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