Xanthine dehydrogenase electrocatalysis: autocatalysis and novel activity.

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Kalimuthu P, Leimkuhler S, Bernhardt PV

Xanthine dehydrogenase electrocatalysis: autocatalysis and novel activity.

J Phys Chem B. 2011 Mar 24;115(11):2655-62. doi: 10.1021/jp111809f. Epub 2011 Mar 1.

PubMed ID

21361328 [ View in PubMed

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Abstract

The enzyme xanthine dehydrogenase (XDH) from the purple photosynthetic bacterium Rhodobacter capsulatus catalyzes the oxidation of hypoxanthine to xanthine and xanthine to uric acid as part of purine metabolism. The native electron acceptor is NAD(+) but herein we show that uric acid in its 2-electron oxidized form is able to act as an artificial electron acceptor from XDH in an electrochemically driven catalytic system. Hypoxanthine oxidation is also observed with the novel production of uric acid in a series of two consecutive 2-electron oxidation reactions via xanthine. XDH exhibits native activity in terms of its pH optimum and inhibition by allopurinol.

DrugBank Data that Cites this Article

Drug Enzymes

Drug	Enzyme	Kind	Organism	Pharmacological Action	Actions
NADH	Xanthine dehydrogenase/oxidase	Protein	Humans	Unknown	Substrate	Details