Sequence of the gene encoding type F neurotoxin of Clostridium botulinum.
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East AK, Richardson PT, Allaway D, Collins MD, Roberts TA, Thompson DE
Sequence of the gene encoding type F neurotoxin of Clostridium botulinum.
FEMS Microbiol Lett. 1992 Sep 15;75(2-3):225-30.
- PubMed ID
- 1398040 [ View in PubMed]
- Abstract
Primers designed to conserved regions of botulinum and tetanus clostridial toxins were used to amplify DNA fragments from non-proteolytic Clostridium botulinum type F (202F) DNA using polymerase chain reaction technology. The fragments were cloned and the complete nucleotide sequence of the gene encoding type F toxin determined. Analysis of the nucleotide sequence demonstrated the presence of an open frame encoding a protein of 1274 amino acids, similar to other botulinum neurotoxins. Upstream of the toxin gene is the end of an open reading frame which encodes the C-terminus of a protein with homology to non-toxic-non-hemagglutinin component of type C progenitor toxin.