A novel GTP-binding protein hGBP3 interacts with NIK/HGK.
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Luan Z, Zhang Y, Liu A, Man Y, Cheng L, Hu G
A novel GTP-binding protein hGBP3 interacts with NIK/HGK.
FEBS Lett. 2002 Oct 23;530(1-3):233-8.
- PubMed ID
- 12387898 [ View in PubMed]
- Abstract
A novel human guanylate-binding protein (GBP) hGBP3 was identified and characterized. Similar as the two human guanylate-binding proteins hGBP1 and hGBP2, hGBP3 has the first two motifs of the three classical guanylate-binding motifs, GXXXXGKS (T) and DXXG, but lacks the N (T) KXD motif. Escherichia coli-expressed hGBP3 protein specifically binds to guanosine triphosphate (GTP). Using a yeast two-hybrid system, it was revealed that the N-terminal region of hGBP3 binds to the C-terminal regulatory domain of NIK/HGK, a member of the group I GCK (germinal center kinase) family. This interaction was confirmed by in vitro glutathione-S-transferase (GST) pull-down and co-immunoprecipitation assays.