Cloning and expression patterns of two members of a novel protein-kinase-C-related kinase family.
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Palmer RH, Ridden J, Parker PJ
Cloning and expression patterns of two members of a novel protein-kinase-C-related kinase family.
Eur J Biochem. 1995 Jan 15;227(1-2):344-51.
- PubMed ID
- 7851406 [ View in PubMed]
- Abstract
The cDNA clones for two members of a novel protein kinase family were isolated and sequenced. These protein-kinase-C-related kinases, PRK1 and PRK2, display extensive identity to each other, revealing non-kinase domain similar regions. HR1 and HR2. HR1 contains a motif repeated three times (HR1a-c), while HR2 shows similarity to the amino-terminal sequence of protein-kinase-C epsilon and eta isotypes. Both PRK1 and PRK2, expressed in COS 1 cells, are autophosphorylated in immunoprecipitates, indicating intrinsic kinase activity. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues.