Identification of cDNAs encoding two G protein-coupled receptors for lysosphingolipids.
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An S, Bleu T, Huang W, Hallmark OG, Coughlin SR, Goetzl EJ
Identification of cDNAs encoding two G protein-coupled receptors for lysosphingolipids.
FEBS Lett. 1997 Nov 17;417(3):279-82.
- PubMed ID
- 9409733 [ View in PubMed]
- Abstract
The structural similarity of lysosphingolipids to lysophosphatidic acid (LPA) prompted a sequence-based search for lysosphingolipid receptors using cDNA sequence of the Edg2 human LPA receptor. Two closely related G protein-coupled receptors, rat H218 and human Edg3, are highly similar to Edg2. When overexpressed in Jurkat cells, H218 and Edg3 activated serum response element-driven transcriptional reporter gene in response to sphingosine 1-phosphate (S1P), dihydro-S1P and sphingosylphosphorylcholine, but not to LPA. H218 and Edg3 expressed in Xenopus oocytes conferred responsiveness to S1P and dihydro-S1P in agonist-triggered 45Ca2+ efflux. Therefore, H218 and Edg3 are functional receptors for S1P and perhaps other closely related lysosphingolipids.