Tricyclic antidepressant drugs affect histamine receptors in human leukocytes.

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Sadava D, Wilmington K

Tricyclic antidepressant drugs affect histamine receptors in human leukocytes.

Life Sci. 1984 Dec 17;35(25):2545-8.

PubMed ID

6096660 [ View in PubMed

]

Abstract

Tricyclic antidepressant drugs bind to histamine receptors in rodent and other mammalian tissues. However, a readily accessible, normal human tissue for studies of these drugs has not been available. We showed that freshly isolated human leukocytes can be used for this purpose. Following isolation by dextran sedimentation, leukocytes were incubated for 30 sec. in histamine concentrations from 1.0 nM to 1.0 mM. A dose-related increase in intracellular cyclic AMP was observed. When the cells were preincubated for 10 min. in the tricyclic, nortriptyline, and then challenged with histamine, the concentration of histamine needed for a comparable cyclic AMP increase was elevated 100-fold over non-preincubated cells. These results indicate that the tricyclic drug interacts with histamine receptors on leukocytes, and that these cells may be used for biochemical and clinical studies of these drugs.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Nortriptyline	Histamine H1 receptor	Protein	Humans	No	Antagonist	Details