A comparison of the effect of anticancer drugs, idarubicin and adriamycin, on soluble chromatin.

Article Details

Citation

Zahraei Z, Rabbani-Chadegani A

A comparison of the effect of anticancer drugs, idarubicin and adriamycin, on soluble chromatin.

Eur J Pharmacol. 2007 Dec 1;575(1-3):28-33. Epub 2007 Jul 31.

PubMed ID
17716648 [ View in PubMed
]
Abstract

The biological activity of an anticancer agent is related to its physicochemical interaction with biological receptors. In the present study we have investigated and compared the affinity and mode of action of two potent anticancer drugs, adriamycin and idarubicin on soluble chromatin using ultraviolet/visible and fluorescence spectroscopy, hydroxyapatite (HAP) chromatography and gel electrophoresis techniques. The results show that addition of various concentrations of drugs to chromatin solution individually, reduced both absorbance and fluorescence emission intensity of chromatin and precipitated it in a dose dependent manner, however, the extent of reduction was different for two drugs used. This effect was also observed on the histone gel patterns of the drug treated samples revealing that the chromatin is less affected by idarubicin compared to adriamycin implying higher aggregation of chromatin with the former. As hydroxyapatite chromatograms show, histone H1 represented the highest drug binding activity. The results suggest that although adriamycin and idarubicin are both grouped anthracycline antibiotic anticancer drugs, they differ considerably on their binding affinity to cellular chromatin.

DrugBank Data that Cites this Article

Drug Targets
DrugTargetKindOrganismPharmacological ActionActions
IdarubicinDNANucleotideHumans
Yes
Intercalation
Details