Calbindin D28K interacts with Ran-binding protein M: identification of interacting domains by NMR spectroscopy.
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Lutz W, Frank EM, Craig TA, Thompson R, Venters RA, Kojetin D, Cavanagh J, Kumar R
Calbindin D28K interacts with Ran-binding protein M: identification of interacting domains by NMR spectroscopy.
Biochem Biophys Res Commun. 2003 Apr 18;303(4):1186-92.
- PubMed ID
- 12684061 [ View in PubMed]
- Abstract
Calbindin D(28K) is an EF-hand containing protein that plays a vital role in neurological function. We now show that calcium-loaded calbindin D(28K) interacts with Ran-binding protein M, a protein known to play a role in microtubule function. Using NMR methods, we show that a peptide, LASIKNR, derived from Ran-binding protein M, interacts with several regions of the calcium-loaded protein including the amino terminus and two other regions that exhibit conformational exchange on the NMR timescale. We suggest that the interaction between calbindin D(28K) and Ran-binding protein M may be important in calbindin D(28K) function.